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Germinating Pea seeds biology lab

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Purpose: To find evidence of respiration in germinating seeds. Background: Cellular respiration, also known as 'oxidative metabolism', is one of the key ways a cell gains useful energy. It is the set of the metabolic reactions and processes that take place in organisms' cells to convert biochemical energy from nutrients into adenosine triphosphate (ATP), and then release waste products. The reactions involved in respiration are catabolic reactions that involve the oxidation of one molecule and the reduction of another. After a seed drops from a plant it usually goes into a resting period called dormancy when it metabolizes stored energy reserves very slowly. Dormancy is defined as a state during which the seed is not able to germinate. ...read more.


However, seeds respire at a lower rate throughout dormancy. When plants use sugars stored in their leaves or seeds they undergo cellular respiration Sugar + Oxygen --> Carbon dioxide + Water + Energy (ATP) Bromothymol blue is a chemical indicator for weak acids and bases. The chemical is also used for observing photosynthetic activities or respiratory indicators (turns green then yellow as CO2 is added). In this experiment we will be using Bromothymol blue to determine if CO2 is present or not. Hypothesis: The germinating pea seed will undergo respiration where as the dormant pea seeds will not. Independent Variable: Pea Seeds Dependent Variables: CO2 released Control: No seeds Materials: 3x small test tubes, 3x 125ml Erlenmeyer flask, Dormant Pea Seeds, Germinating Pea seeds, Parafilm, Bromothymol Blue, Small beaker. ...read more.


10. Observe and find evidence of respiration. 11. Record data. The end color of BTB with different seeds BTB Color Germinating Seeds Dormant Seeds No seeds Blue X X Yellow X Evauluations: The rate of respiration could not be determine, however we do know that respiration was occurring in the geminating seeds. Limitations: Abiotic factors were not considered on the rate of respiration. We could not measure the amount of CO2 release by the germinating seeds thus we could not create a general knowledge of the rate of respiration by the germinating seeds. Conclusion: The germinating seeds were the only seeds that showed sign of respiration. This was determined by the yellow color of the BTB solution which indicates CO2 gas present. Dormant seeds had no evidence of respiration because the BTB solution remained the same color. The flask with no seeds had no respiration because there was no organism present to under cellular respiration. ...read more.

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