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Growing Bacteria collected from surfaces and observing the results.

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Bacteria Microbiology Francis, Chardai March 13, 2009 Mr. Toulka & Ms. Bove Period 1 Objective: What I learned from the Bacteria Microbiology experiment is how to culture bacteria. If somebody was to whip any common surface with a cotton swab, then whip the agar in a Petri dish with the newly infected cotton swab and leave it over night in an incubator, bacteria would start to grow. Also, I learned how to isolate bacteria to try to get a single cell by its self, which is done by using an inculcating loop, a new Petri-dish with agar, and an alcohol burner. I learned how to indentify bacteria, which is done by using a gram stain, observing how the bacteria move, what shape and size they are, and where they are found. I even realized how hard it is to be a scientist and how they should have a lot of patience because when I was using the micscrope to look at the bacteria with the oil immersion, I was getting annoyed very quickly because how long of a process it is to focus and actually find what you're looking for. ...read more.


3. Using the sterile loop, streak cultures over one fourth of the surface of the agar plate, Then flame loop 4. Cool loop, pass the cooled the loop three or four times over intial streaked portion of the plate. Streak it without over lapping the next quadrant. 5. Flame loop and allow it to cool 6. Pass the loop over the streak portion of the second quadrant two or three times a and then streak materials without over lapping over the third quadrant of the plate 7. Repeat step 6 to streak last quadrant 8. Flame loop before you put it down. Finished II. Identification A. Bacterial Morphology * To be entered in your data section, not your procedure. Type of margin, elevation, pigmentation, texture, light transmission B. Gram Stain * Make a bacterial smear * Place bacterial smear in tray. Cover the smear with Crystal violet for about two minutes. (Use forceps for this procedure to keep the reagents off your skin) * Place slide in Gram's Iodine tray for about 1-2 minutes * Remove * Decolorize by washing slide under alcohol * Cover with Safranin counter stain for two minutes * Rinse with water blot excess without rubbing smear * Dry C. ...read more.


Materials: -inoculating loop -gram stains (Crystal violet, Gram Iodine) -Microscope -Petri-dish -Agar -Alcohol burner -incubator Data and Observations: I observed that the bacteria grow plenty considering we didn't culture it very long, only over night. The bacteria had a bad smell. It wasn't colorful it was just an off whitish color. It mostly grew only on the spot where I rubbed it. It wasn't liquidly, it was more of solid. In the sensitivities portion of the experiment the hand sanitizer and the ampillin were the only two things that are resistant to the E-coli. They weren't close together either. Analysis & Conclusion: Since we used the incubator the bacteria grew, or cultured, faster than if we just let it sit out in a room temperature room. Also, the incubator controlled the bacteria's growth by controlling how much heat the cultured bacteria will get and how often. If I was to do anything different I would let the bacteria sit in a room temperature room, to see how fast bacteria grows on its own. I now know that I shouldn't put my hands in my mouth so much and that I should wash my hands more often because a lot of common surfaces are infected with nasty bacteria. ...read more.

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