• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month
Page
1. 1
1
2. 2
2
3. 3
3
4. 4
4

# How amylase concentration affects the rate of the starch concentration

Extracts from this document...

Introduction

How amylase concentration affects the rate of the starch concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration: In a more concentrated solution, collision occurs more often because there are more molecules. The more often these molecules collide, the greater the chance they having of reacting. This means that the rate of a chemical reaction will increase if the concentration of reactants is increased. However, in this experiment, I'm going to do an investigation to find out how amylase concentration affects the rate of starch Hypothesis: In this investigation, the concentration of enzyme will be inversely proportional to the time taken for starch to be digested, until at a certain point where it will level out. It will level out because, all the substrates would have been used up, therefore there will be no more substrates for the enzymes to work on. In effect, the concentration of the substrate will act as a limiting factor. ...read more.

Middle

Stop it when you have readings for 5 different starch concentrations. 8)- Plot a graph of absorbance against starch concentration. Key variables: In this experiment, I will use the same amylase concentration and quantity through out the experiment and the same starch concentration and quantity through out the experiment. The temperature would be kept constant and at a reasonable temperature of 37oc to ensure that the enzymes are acting at their maximum and at the same time do not get denatured. I will add the same drops of iodine solution Evaluation of Method: A)-This experiment measures how the different concentration of Amylase affects the rate of starch concentration; this is a valid measure of an enzyme digest starch and the relationship between them. The apparatus chosen is suitable because it enabled the digestion of starch to be measured by the absorbance of the solution. B)-Possible safety problems with this experiment are shown on the risk assessment sheet. Risk Assessment: -I will wash away any spillage on my skin with water to avoid skin irritation. -I will wear safety goggle through out the experiment. -I will clear up any breakage or spillage that occurs using a hand glove to prevent Glass cuts. -I intend using an already made amylase solution, but if eventually I have to make It by myself, I will avoid inhaling the powder (amylase enzyme). ...read more.

Conclusion

Also, this method was inaccurate because the number of drops of starch and amylase mixture that was added to the iodine solution was varied. In the method the pupils should be told the exact quantity of drops that should be added to the iodine solution. These changes and suggestions, could lead to a better result with no anomalies. If I were to do this experiment again, it would be done in a different way. I would repeat the experiment more than 6 times in each concentration to ensure that the results obtained are accurate. Apart from the one or two anomalies I had, I think the results were accurate because they showed the relevant pattern and supported my prediction as well as my conclusion. With the curve of average points in the middle of the both readings, I can say that my results were reliable and were enough evidence to support my conclusion. Significance of Results: When commenting on the trend shown, the degree of error must be taken into consideration. Discussion: The hypothesis is the experiment was: The amylase concentration increased. And it is supported when the results are analysed. Conclusion: This investigation has proved that the greatest amount of amylase in a concentration has the most positive effect on the breakdown of starch, whereas the least amount of amylase has the opposite effect. ?? ?? ?? ?? HABIB DAHEL Biology coursework 2 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

## Found what you're looking for?

• Start learning 29% faster today
• 150,000+ documents available
• Just £6.99 a month

Not the one? Search for your essay title...
• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month

# Related AS and A Level Molecules & Cells essays

1. ## How does the concentration of enzymes affect the breakdown of starch by a-amylase in ...

4 star(s)

a very small period of time, if compared to the first method). Secondly, upon obtaining the results, when adding the iodine solution, a circle of faded colour will be seen where the starch has been broken down. This circle can be measured in diameter, and this measurement can be used as values for the rate of breakdown.

2. ## The effect of amylase concentration on the breakdown of starch

3 star(s)

pH Level:- This is again like temperature in the way that every enzyme has an optimum temperature the same as they have an optimum pH. Optimum means the "best" or in this case the best conditions for the enzyme to break down the starch the quickest.

1. ## For this investigation I am going to investigate the effect of the concentration of ...

This meaning all the concentrations of amylase will be working on the same amount of starch. Inhibitors Competitive inhibitors compete with the substrate for the same active sites on the enzyme this means the reaction will be slower as the starch will have less active sites to go into, where

2. ## Experiment to Investigate the Effect of Copper Ions on a Solution of Amylase and ...

This is since there will be a greater number of active sites available for the starch molecules. Therefore there will a greater number of successful collisions per second and the rate of reaction will increase. 5. Iodine concentration and volume- 2 drops of 0.1M of iodine will placed in each

1. ## How the concentration of amylase effects the digestion of the starch.

This is also know as denaturing the enzyme, but unlike the increase in temperature the increase in PH level would temporarily damage the enzyme, where as the increase in temperature would disable the enzyme forever. The diagram above is from a text book...

2. ## Investigating the Rate of Reaction of the Enzyme Amylase on starch

Number of collisions With Kinetic Energy, E Activation energy Kinetic Energy (E) The diagram above shows that when the concentration of the solution is 3%, there are few molecules that have the kinetic energy needed for a reaction to occur.

1. ## Investigate How The Concentration Of Amylase Affects The Rate Of Reaction Between Starch And ...

Then added the solution into each well according to the time span. Then I recorded my results. Results Add one drop of Add one drop of Add one drop of Starch alone to Starch 1% amylase Starch 1% amylase This well.

2. ## Catalyse Investigation

The amount and time this acid was added was kept constant for each reading and so will not affect the accuracy of the results. iii) Substrate Concentration - When there is an excess of enzyme molecules, an increase in the substrate concentration, produces a corresponding increase in the rate of reaction.

• Over 160,000 pieces
of student written work
• Annotated by
experienced teachers
• Ideas and feedback to