How amylase concentration affects the rate of the starch concentration

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HABIB DAHEL                                                                                 Biology coursework

 

How amylase concentration affects the rate of the starch concentration

In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells.

However, the rate at which enzymes work are affected by the following factors/variables:

Concentration: In a more concentrated solution, collision occurs more often because there are more molecules. The more often these molecules collide, the greater the chance they having of reacting. This means that the rate of a chemical reaction will increase if the concentration of reactants is increased.

However, in this experiment, I'm going to do an investigation to find out how amylase concentration affects the rate of starch

Hypothesis: 

In this investigation, the concentration of enzyme will be inversely proportional to the time taken for starch to be digested, until at a certain point where it will level out. It will level out because, all the substrates would have been used up, therefore there will be no more substrates for the enzymes to work on. In effect, the concentration of the substrate will act as a limiting factor. However, enzyme concentration will be directly proportional to the rate of reaction.

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The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter,

Equipments:

Iodine solution: used to test for present of starch - Amylase solution - 1% starch    solution - 1 pipette - 3 syringes -  

      8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter

 

Method:

Amylase is an enzyme found in ...

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