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How amylase concentration affects the rate of the starch concentration

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Introduction

How amylase concentration affects the rate of the starch concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration: In a more concentrated solution, collision occurs more often because there are more molecules. The more often these molecules collide, the greater the chance they having of reacting. This means that the rate of a chemical reaction will increase if the concentration of reactants is increased. However, in this experiment, I'm going to do an investigation to find out how amylase concentration affects the rate of starch Hypothesis: In this investigation, the concentration of enzyme will be inversely proportional to the time taken for starch to be digested, until at a certain point where it will level out. It will level out because, all the substrates would have been used up, therefore there will be no more substrates for the enzymes to work on. In effect, the concentration of the substrate will act as a limiting factor. ...read more.

Middle

Stop it when you have readings for 5 different starch concentrations. 8)- Plot a graph of absorbance against starch concentration. Key variables: In this experiment, I will use the same amylase concentration and quantity through out the experiment and the same starch concentration and quantity through out the experiment. The temperature would be kept constant and at a reasonable temperature of 37oc to ensure that the enzymes are acting at their maximum and at the same time do not get denatured. I will add the same drops of iodine solution Evaluation of Method: A)-This experiment measures how the different concentration of Amylase affects the rate of starch concentration; this is a valid measure of an enzyme digest starch and the relationship between them. The apparatus chosen is suitable because it enabled the digestion of starch to be measured by the absorbance of the solution. B)-Possible safety problems with this experiment are shown on the risk assessment sheet. Risk Assessment: -I will wash away any spillage on my skin with water to avoid skin irritation. -I will wear safety goggle through out the experiment. -I will clear up any breakage or spillage that occurs using a hand glove to prevent Glass cuts. -I intend using an already made amylase solution, but if eventually I have to make It by myself, I will avoid inhaling the powder (amylase enzyme). ...read more.

Conclusion

Also, this method was inaccurate because the number of drops of starch and amylase mixture that was added to the iodine solution was varied. In the method the pupils should be told the exact quantity of drops that should be added to the iodine solution. These changes and suggestions, could lead to a better result with no anomalies. If I were to do this experiment again, it would be done in a different way. I would repeat the experiment more than 6 times in each concentration to ensure that the results obtained are accurate. Apart from the one or two anomalies I had, I think the results were accurate because they showed the relevant pattern and supported my prediction as well as my conclusion. With the curve of average points in the middle of the both readings, I can say that my results were reliable and were enough evidence to support my conclusion. Significance of Results: When commenting on the trend shown, the degree of error must be taken into consideration. Discussion: The hypothesis is the experiment was: The amylase concentration increased. And it is supported when the results are analysed. Conclusion: This investigation has proved that the greatest amount of amylase in a concentration has the most positive effect on the breakdown of starch, whereas the least amount of amylase has the opposite effect. ?? ?? ?? ?? HABIB DAHEL Biology coursework 2 ...read more.

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