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How can the size of microscopic specimens be estimated?

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Introduction

Liban Ahmed                                   Experiment 2                           September 20, 2003

Activity 2: Estimating Size Using a Microscope

Problem

How can the size of microscopic specimens be estimated?

Hypothesis

The size of microscopic specimens can be estimated by using a miniature plastic ruler that can measure millimeters and determining the diameter of the field of view.

Materials

  • Prepared slides of human tissue
  • Microscope
  • Clear plastic ruler that can measure millimeters

Procedure

  1. Made tables in notebook to record the field of vision and cell dimensions under low, medium, and high magnifications.
  2. Brought a microscope to the lab area making sure to use both hands to carry the microscope and that the cord was not dangling.
  3. Looked through the instructions making sure there were no problems that could be posed.
  4. Organized the lab area and prepared all equipment including slides and the microscope.
  5. Switched to the low-power objective lens.
...read more.

Middle

Magnification

Field of View ( Measured)

mm                                          µm

Filed of View (Calculated)

mm                                              µm

Low

4.3                                         4300

n/a                                                n/a

Medium

1.5                                         1500

1.72                                           1720

High

n/a                                            n/a

0.375                                           375

Cell Dimension

Magnification

Cell Length

...read more.

Conclusion

  1. The cell dimensions remained the same under the low, medium, and high magnifications. This does make sense because increasing or decreasing the magnification of an object does not make the object larger or smaller, it just allows the individual to observe more or less of the object. Therefore, the cell dimensions should have remained more or less the same because the magnification does not affect the dimensions of the object.
  1. If a cell were found to be 8 µm in length under the low-power magnification, it would be 8 µm in length under the high-power magnification. This is due to the fact that the magnification of a microscope does not affect the dimensions of an object.
  1. I would estimate the dimensions of the cell to be 0.6 mm (600 µm) in length and 0.3 mm (300 µm) in width.  

...read more.

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