Factors:
All of these factors could affect my investigation results these are:
- Temperature
- Concentration of the hydrogen peroxide.
- Volume of yeast.
- Volume of hydrogen peroxide
- Blockers: this is where a competitive inhibitor goes into the active site of the enzyme thereby preventing the substrate from binding with the enzyme.
- Its pH
My chosen factor:
The factor which I have chosen to do is the different temperatures which I react the two chemicals together.
Prediction:
I think that the higher the temperature is the faster the reaction will take place also the troth will get bigger when the chemicals are at a higher temperature.
Though if the chemicals get too hot then this will denature (make the enzymes change shape) and there will be no reaction between the hydrochloric acid and the yeast. The more enzyme molecules, the faster the reaction will proceed provided there are enough substrate molecules provided.
The temperature that I think will have the biggest reaction will be around 43 .
How I think the results will turn out like.
- A = The molecules have more energy so therefore they move around more and have more collisions the more of a chance there is to for a reaction to take place.
- B = it has reached its optimum temperature of the reaction.
- C = reaction speed slowing down as the temperature is too hot and the enzymes start to denature.
Theory
From my prediction, I am going to explain the process of the reaction in more detail.
For a reaction to take place there has to be a collision. In the experiment the substrate (hydrogen peroxide) fits into the enzyme (yeast) and the enzyme breaks up the substrate into smaller molecules. This is called the lock and key theory.
This process is called a catabolic reaction this means a break down in reaction. A rise in temperature of most chemical reactions. In many cases a rise of 10 C will double the rate of reaction in a cell. Above 50 C the enzymes start to denature (loose their shape) and eventually stop working.
Only one type of enzyme can work with one Substrate because of the lock and key theory.
