• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How does Temperature affect the Rate of Respiration in Wax Worms?

Extracts from this document...


Biology AS Coursework How does Temperature affect the Rate of Respiration in Wax Worms? P I A E spg Total Mark = Name: Daniel Comey Candidate Number: ? Centre Number: ? Aim The aim of this investigation is to determine what affect temperature has on the rate of respiration of a simple organism. The simple invertebrate organisms I will be using in this investigation are wax worms. Theory To convey all the background research I will need in order to make a valid hypothesis I will split my theory section into the following sub-headings: * Background information on wax worms * Respiration in invertebrates * How temperature affects respiration * The Quotient 10 law Background information on wax worms Wax worms (Galleria Mellonella), in their natural environment, are very destructive pests of honeycombs. They are now bred and used for fish bait and to feed exotic pets, for example reptiles. The greater wax moth (an adult life stage of a wax worm) will lay their tiny white eggs near a beehive. There they will develop into wax worms (larvae stage) and tunnel their way though weak honey beehives. There they will feed on honey, beeswax and honeycomb leaving a mass of debris, silken threads and damaged honeycomb frames. ...read more.


I will carry out the experiment by placing three wax worms in a boiling tube that is attached to a respirometer and placing the boiling tube into the water bath at the temperature that I am testing. Then I can repeat this process over different temperatures to test all the temperatures that I am interested in testing the rate of respiration for. I will also repeat the whole process a second time to get an average for my results. For my results to be significant in any form I must make this a fair test. To make a test fair all the variables must be kept constant apart from the variable that is being investigated. To make this a fair test I will have a control boiling tube hooked up to a respirometer to make sure that the change in the respirometer is due to the wax worms and nothing else. Time is also a varying factor so I will time the boiling tubes, five minutes should be a sufficient amount of time to notice a change in oxygen levels. I have already decided to use a time limit of five minutes per temperature. The temperatures that I will test the rate of respiration for will be 10�, 20�, 30�, 40�. ...read more.


I am aware that if I had one 'off' experiment my results could be completely different from what they are supposed to be. This is why I carried out the experiment twice, to get an average so my results were more accurate and so that the results could not have been the result of an 'off' experiment. My two sets of data are very similar and it is very unlikely that I had two 'off' experiments. The majority of the equipment that I used was accurate. One exception was the water bath. I chose to use a beaker and thermometer water bath for convenience reasons; if I had used a thermostatically controlled water bath the temperature would have been more constant and my results may have been more accurate. There were some bubbles present in the manometer fluid when I carried out the experiment, this may have made my results slightly inaccurate. Also there are probably more sophisticated respirometers that I could have used (a digital one, for example), to get more accurate results, however I do not think this would make such a significant difference. To improve this investigation I could use the equipment above, maybe different time (longer times), although different temperatures would not be a good idea as the wax worms looked uncomfortable at 40�C. Daniel Comey Biology Coursework ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Energy, Respiration & the Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Energy, Respiration & the Environment essays

  1. Marked by a teacher

    effect of temperature on the rate of respiration in yeast

    5 star(s)

    Therefore the rate of respiration will increase as more substrate will be available for active sites and more enzyme substrate complex will occur. 7) Batch of yeast: the same batch of yeast should be same through out the investigation whether this can affect the activity of yeast.

  2. Marked by a teacher

    'An investigation into the ability of two strains of the yeast Saccharomyces cerevisiae to ...

    4 star(s)

    To ensure that accuracy is maintained, a 25cm3 volumetric pipette and filler are used to remove 25cm3 of each solution. These samples should be placed in the corresponding appropriately labelled flasks to hand. Between the removal of each sample, the pipette must be flushed through with distilled water to ensure that contamination is avoided.

  1. Yeast Coursework

    Average amount of CO2 produced (cm3) 20 0.25 30 0.00 40 2.50 50 13.00 60 11.00 Adding the two results for each test, and then dividing it by two found the above results. The rate of respiration is found by dividing the average amount of carbon dioxide produced by the

  2. the effect of bile concentration on the activity of the enzyme lipase during the ...

    impact on the data because the percentage error cause by using the measuring cylinder is only 2%. This means that because of the low accuracy of the glassware it has a 2% possibility of altering our results. Use a pipette to measure the amount of milk which is more accurate.

  1. An investigation into the effects of temperature on the rate of anaerobic respiration of ...

    After a period of time, the rate of respiration decreases after the substrate molecules are used up and many of the active sites are full. Although the carbon dioxide production increases fastest by the yeast solution in the beaker of water at 50 �C, by 50 minutes, each gradient was

  2. How does the temperature of the water in rock pools affect the distribution of ...

    and distribution (the number of dog whelks in the rock pool). 'However whereas it is commonly reported that the distributions of organisms are correlated with temperature, the causality of the phenomenon is not always clear' 5 There are several reasons for this, including a change in feeding and a possible reaction to change in oxygen levels.

  1. Investigate the effect of bile salt concentration on the digestion of milk by the ...

    The bile salts lower the surface tension of the fat globules and thus act to break the lipids into smaller micelles which allow hydrolysis to occur quickly so this fluctuation in pH doesn't occur. This is so that the pH in the small intestine remains fairly constant at the optimum

  2. Investigation into how Lichen growth is affected as you move further away from a ...

    I then totalled the number of different pairs of data. I then multiplied this value by the total pairs of data squared minus 1, which gave me the value for the bottom part of the data. I then divided the vale for the top part by the value for the bottom part of the data.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work