Prediction
I predict that the higher the temperature of the test tube mixture, the quicker the gelatin will be broken down. I predict this because enzymes move quicker in a warmer mixture.
Plan
Apparatus
Thermometer
A 25ml glass beaker
4 test tubes
Wire gauze
Tripod
Bunsen burner
4 pieces of camera film
2cm trypsin
Method
We will get camera film containing gelatin and get 4 test tubes filled up 2cm with trypsin. Then get the test tubes at the right temperature. The first one at
0 C, the second 20 C, the third 40 C and the fourth at 60 C. Then put an equal amount of camera film in each test tube, then to use a stopwatch to time this, watch these test tubes and when the camera film goes clear you will know that the glatin has dissolved, stop the stopwatch and record the time it took. Repeat this three times.
Fair test
To make sure that this is a fair test I will use the same amount of camera film in each experiment.
Safety
To keep safe during this test wear safety glasses at all times, as I will be handling chemicals. Stand up so that if the experiment fell over I would be able to get away from the hot chemicals quickly.
Results
Conclusion
The pattern in my graph is that the time that the camera film took to dissolve is quite a long time at the start (5 C), but as we got closer to 40 C it started to dissolve quicker, then once past 40 C it starts to take a longer time again. I think that this so because the enzymes work best at body temperature (37.5 C), this is because enzymes are found in your saliva.
These results support my original prediction in one way as I said that the higher the temperature, the quicker the gelatin dissolves. I was also right as when the temperature started to get higher it started to take less time to dissolve, but after 40 C it started to take longer which I didn’t think would happen so I was also wrong.
Evaluation
I think that my results and experiment went well, but for some of the times I did the experiment I forgot to heat up the enzymes before I put the camera film in, so if I were to do this experiment again then I would remember to heat up the enzymes before putting in the camera film. This would improve my results.
Another way that I could improve this experiment is to do the experiment at more varied temperatures.
