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How does temperature affect the way in which an enzyme works in Hydrogen Peroxide?

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How does temperature affect the way in which an enzyme works in Hydrogen Peroxide? Introduction. The aim of this investigation is to find out how temperature affects the speed at which the enzyme works best in Hydrogen Peroxide. I hope to achieve reliable results that will confirm my predictions. I predict that: - � By increasing the temperature of the Hydrogen Peroxide, the enzyme will react faster. This is because the particles have more energy so they will move and react at a faster rate. � At about 40c, the enzyme will start to deteriorate because the substrate no longer fits the enzyme making the enzyme useless. � I predict that the enzyme will work at its best at 37c because that is the human body temperature. � With temperatures lower than 40c,I will expect the rate of reaction to increase gradually as the temperature is increased because the particles will move faster because of the increase of their energy therefore they will react faster. � With temperatures higher than 40c,I expect to see the rate of reaction decrease rapidly and the enzyme become denatured because the enzyme and substrate no longer fit together meaning the enzyme is denatured. � I also predict that when my results are put on to a graph they will look something like this... ...read more.


reflects the general effect of increasing temperature on the rate of chemical reactions (graph below). The descending portion of the curve above (blue arrow) reflects the loss of catalytic activity as the enzyme molecules become denatured at high temperatures. An enzyme is a biological catalyst, which alters the rate of reaction without being changed itself. Enzymes are proteins; they have very precise three-dimensional shape, which forms a one specific active site on the enzyme. Each enzyme can only convert one kind of substrate molecule in to one kind of product molecule. These are specific. Hypothesis. During preliminary work, I tried two different methods. These methods were: � Counting the number of bubbles produced during a time limit of two minutes. And � Timing how long it took the enzyme ball to reach the top of the hydrogen peroxide after dropping t I decided to use the ball resurfacing method because I believe it would be more accurate and if I miss any bubbles it could affect my whole experiment. In addition, I thought that the bubble counting method is simple and effective but wouldn't be as accurate than the other method I tried. By timing resurface, the rate of reaction is shown more clearly as the temperature is increased and decreased over a range of temperatures. ...read more.


This might have affected the results that were obtained. In addition, instead of using the resurface timing method, a gas syringe could have been used to see how much oxygen was given off. This would have given a much more accurate result than the results obtained. By putting the hydrogen peroxide in a water bath, the hydrogen peroxide would have stayed at the same, constant temperature throughout the experiment. Unfortunately, there were not enough water baths, so we had to heat up the hydrogen peroxide to the desired temperature and then carry on with the experiment, which meant that the temperature of the hydrogen peroxide fluctuated during the experiment, which more than likely affected the overall results. In addition, the hydrogen peroxide was measured by a plastic rule, which meant that the amount of hydrogen peroxide might not have been the exact amount. If an electronic liquid measurer had been used, the amount of hydrogen peroxide would have been more accurate and also the results may have been more accurate. --------------------------------------------------------------------------------------------------------- Out of the two methods used, the counting bubbles method was probably the most reliable because it would of been clear and you could tell that gas was actually being produced because the bubbles would be very easy to see. I think this experiment was a fair test because it was timed each time with an accurate stopwatch. I am very pleased with the results I obtained. ...read more.

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