• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

I am going to investigate the rate of reaction between catalase and hydrogen peroxide.

Extracts from this document...

Introduction

Catalase Investigation. I am going to investigate the rate of reaction between catalase and hydrogen peroxide. Background: After researching in CD-ROMs, text-books, and the internet, I have found the following information that may also be relevant to this project: Hydrogen Peroxide: Hydrogen Peroxide has a formula of H[2]O[2], being a chemical compound of hydrogen and oxygen. It is a clear, colourless, syrupy liquid (though water-like in appearance). Hydrogen peroxide is made as a by-product of some chemical reactions that take place in our cells. One place this is particularly common is in the liver. Hydrogen Peroxide will blister the skin if in contact. It decomposes very slowly at room temperature, but rapidly in water and oxygen when magnesium oxide is added. Pure H[2]O[2] liquid may explode violently if heated to a temperature above 100�C, which proves that concentrated solutions are unstable. Being aware to this, I will need to take necessary precautions; Goggles must be worn when handling H[2]O[2], and special care not to spill any on the skin (plastic gloves may be needed). The liquid solidifies at -0.41�C and is non-flammable at any concentration. It is soluble in water and the usual commercial concentrates available are 3% and 30% aqueous solutions. To slow down the decomposition of H[2]O[2] into water and oxygen, it is kept in dark bottles at low temperatures, and organic substances like acetanilide, are added to the solutions. H[2]O[2] acts as a reducing and oxidizing agent (redox). The bleaching of substances (hair, ivory, feathers, delicate fabrics, paper, pulp etc), which would be destroyed by other agents, are carried out using H[2]O[2]'s oxidising properties. ...read more.

Middle

conclusion that that may be inaccurate, as the bubbles may be different sizes, and we cannot establish a relative unit for measuring the total amount. So we decided to use an up-turned measuring cylinder, filled with and in a tub of water, with the tube underneath. This way we can accurately measure the oxygen and give recognisable units. (See Page 5 For diagram). In the experiment I will record the amount of oxygen gas produced every 15 seconds so that I can see if the speed the gas being made changes as the reaction undergoes. For the catalase effect on hydrogen peroxide investigation it would be best to choose one of these continuous variables to study: Volume of catalase enzyme Temperature of surroundings Volume of hydrogen peroxide Consistency of time recorded Strength of hydrogen peroxide [image008.gif] For my experiment my variable is going to be the concentration of hydrogen peroxide (in vol). Required Hydrogen Peroxide solution: 5 vol 10 vol 15 vol 20 vol Hydrogen Peroxide (ml): 2.5ml (25%) 5ml (50%) 7.5ml (75%) 10ml (100%) Water (ml): 7.5ml 5ml 2.5ml 0ml Total Volume (per experiment): 10ml 10ml 10ml 10ml Total Volume of Hydrogen Peroxide (whole experiment): 3 x 2.5 = 7.5 3 x 5 = 15 3 x 7.5 = 22.5 3 x 10 = 30 7.5 + 15 + 22.5 + 30 = 75ml Chemicals and Apparatus needed: Quantity: Potato Homogenate (Catalase enzyme) Hydrogen Peroxide (20 vol) Clamp Stands Bosses Clamps 20ml Syringe 5ml Syringe (to measure catalase) Test tube 100ml Measuring Cylinder (upturned) Plastic Tub Delivery Tube U-tubes Bung (with two holes) Water (to fill tub and measuring cylinder) ...read more.

Conclusion

* The force of the hydrogen peroxide being thrust into the catalase from the syringe will have been different as it was by inaccurate human ways. * The apparatus may have not been airtight (letting some oxygen produced escape). * Oxygen was already in the airtight apparatus before the solution began reacting, so this would obscure results. * When the u-tube was put under the measuring tube some needed oxygen may have accidentally escaped. * The potato homogenate was not pure catalase, so the results were not accurate, in comparison to the hydrogen peroxide. Prototype Apparatus (before) [image010.gif] We considered many of these problems before starting the experiment, so after a few dummy runs using the equipment layout as above, we used trial and improvement and we came up with these solutions: * We put vaseline around any joints, to create an air tight seal. * We swapped the catalase and the hydrogen peroxide around, so that the H[2]O[2] was in the syringe where as it had a larger volume (less chance of getting stuck in the tube). * We changed the syringe tube to vertical (less chance of contents getting stuck). * We used a manageable small amount of catalase to a larger amount of H[2]O[2] (approximately 1:15), which we found that the oxygen production rate was not too fast to measure. * We used a large measuring cylinder (we found that with even small amounts of solution, there is enough oxygen produced to almost fill a 100ml measuring cylinder). If I were to do this experiment again, I would consider all these problems more thoroughly. I would try and get access to more accurate measuring equipment and chemicals. By Rachel Morrell 9Mg [image011.gif] [image012.gif] ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Bubbles can also change the surface area of reactants, so the pouring of solutions will be done in a subtle way to reduce the amount of bubbles and the tests will not be continued until all bubbles are popped inside the boiling tubes and syringes, the 'Equipment' passage explains how to do this.

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    The factors that affect the rate of reaction (decomposition of hydrogen peroxide) are shown below: * Enzyme concentration - the amount of catalase present in celery extract * Substrate concentration - hydrogen peroxide * Temperature * pH level I have chosen to investigate the concentration of the enzyme Catalase.

  1. Reaction of Catalase and Hydrogen Peroxide

    They increase the rate of a reaction without themselves being used up. 3) Some enzymes require another compound called a coenzyme to be bound to them before they can catalyse reactions. 4) Their presence does not alter the nature of the end products of the reaction.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    If the vibration becomes too violent then the chemical bonds in the enzyme break up. As the vibration becomes violent and the chemical bonds break up this means that the enzyme has denatured and changed shape at a high temperature.

  1. Investigating the break down of Hydrogen Peroxide using catalyst

    Hydrogen peroxide is toxic so needs to be changed into harmless substances. This information was found in textbooks and on Encarta where the functions and uses of enzymes, specifically catalyst, were researched. In my investigation I will study the effect of substrate concentration on the rate of catalyst activity.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    I did so in order to be able to focus on the concept of inhibitors in this part of the investigation. Chemical inhibitors are molecules, that have a shape and structure which is very much resembling to the substrate's one.

  1. Catalyse Investigation

    A control was carried out in this investigation to ensure that it was the presence of the enzyme sucrase alone, that was breaking down sucrose to glucose and fructose and that only the effect of temperature was being measured. This was achieved by carrying out the experiment described above for

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    sites are available and hence more substrates can react at any one time therefore increasing the rate of reaction. The graph below shows how rate of reaction alters with increasing concentration, the direct proportionality of the two factors is shown by the straight line intersecting the origin.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work