• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month
Page
1. 1
1
2. 2
2
3. 3
3
4. 4
4
5. 5
5
6. 6
6
7. 7
7
8. 8
8
9. 9
9

I predict that the juice will have at least 2 or more of the same amino acids as the ones in the amino acid mix. I predict that the amino acid more likely to occur is aspartic acid, as lemon juice is acidic.

Extracts from this document...

Introduction

CHROMATOGRAPHY Hypothesis I predict that the juice will have at least 2 or more of the same amino acids as the ones in the amino acid mix. I predict that the amino acid more likely to occur is aspartic acid, as lemon juice is acidic. Method * We first extract some lemon juice from a lemon. Cut the lemon around the equator and squeeze it with a juicer. * Pour the juice into six tubes at approximately the same level each. * Place the tubes in a beaker and weigh them (remembering to tare the beaker first). Make pairs of tubes of equal mass by adding or taking out juice with a clean pipette) and place them opposite each other in the centrifuge and set it to run for 5 minutes. * After centrifuging the juice, pour all the supernatant into a beaker and wash out the solid precipitate from the tubes. * Take 2 samples of the raw centrifuged juice in tubes, label and put them away. * Add alcohol to the remaining juice with the ratio of 1:3 (1unit of juice to 3 units of alcohol). ...read more.

Middle

1st aa: x = 9/154 = 0.058 y = 15/154 = 0.097 z = 1/154 = 0.0065 2nd aa: x = 34/154 = 0.22 y = 43.5/154 = 0.28 z = 21/154 = 0.14 Amino Acid Mix 1st aa: x = 33/154 = 0.21 y = 44.5/154 = 0.29 z = 15.5/154 = 0.10 2nd aa: x = 54.5/154 = 0.39 y = 73/154 = 0.47 z = 48.5/154 = 0.31 3rd aa: x = 80/154 = 0.52 y = 85.5/154 = 0.56 z = 74/154 = 0.48 4th aa: x = 104/154 = 0.68 y = 117.5/154 = 0.76 z = 90/154 = 0.58 Asp. x = 35/154 = 0.23 y = 45.5/154 = 0.30 z = 17/154 = 0.11 Leu. x = 77/154 = 0.50 y = 114/154 = 0.74 z = 60.5/154 = 0.39 Lys. x = 52.5/154 = 0.34 y = 72/154 = 0.47 z = 45/154 = 0.29 Pro. x = 75/154 = 0.49 y = 90/154 = 0.58 z = 64.5/154 = 0.42 Percentage Variation (PV) Juice 1st aa: 20-10/10*100 = 100% 0-10/10*100 = -100% PV = 0.065 +- 100% 2nd aa: 46-33.5/33.5*100 = 37.3% 28-33.5/33.5*100 = -16.4% PV = 0.22 + 37.3% or 0.22 - 16.4% Juice + ...read more.

Conclusion

I had marked what I thought was the darkest region of the spot with an 'x.' However that mark was aligned with the mark for Pro and so indicating that they are the same amino acid. This is impossible, but may have been caused by either: Misinterpreting the darkest region of the spot (marking the wrong place) Accidentally using the same micropipette for two substances It would make more sense if the mark on the spot for Leu was closer to the top of the spot, where it would be aligned to one of the spots in the amino acid mix. The 1st amino acid spot for the juice had a smaller spot within it. The smaller spot even showed up as a different color to that of the larger spot, so it must be just another amino acid, not present in the amino acid mix. I could have improved this experiment by making sure that I do not add too much of a substance onto the chromatogram, by getting a second opinion on whether I had marked the darkest region of a spot or not and/or by using a wider variety of amino acids to identify the other amino acids in the juice which do not align with any others. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Physical Chemistry section.

Found what you're looking for?

• Start learning 29% faster today
• 150,000+ documents available
• Just £6.99 a month

Not the one? Search for your essay title...
• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month

Related AS and A Level Physical Chemistry essays

1. Stereochemistry of Butenedioic acid

5 star(s)

Nucleophilic attacked by bromide anion caused the cyclic bromonium ion ring to open. The net result was the addition of two bromines to the double bond. Procedures: Part A. Conversion of maleic acid to fumaric acid 1. About 2 grams of maleic acid was weighed out in a clean dry 100 cm3 beaker.

2. Finding out how much acid there is in a solution

They are both equally weak therefore, the point of equivalence would be pH 7. Once it reaches the point of equivalence, the curve drops down slightly and then ends like the curve above (strong acid Vs weak base). Weak Acid Vs Strong Base: e.g.

1. An Investigation using Chromatography to determine the different Amino Acids

I put the solvent into the Gas Jar it was just submerged into the solvent. So as I just mentioned I then put the solvent into the bottom of the Gas Jar, so that it could rise up the chromatography paper, put then lid on so that the vapours from

2. Analysis of amino acids by paper chromatography

Origin1 X Origin2 Asp (for aspartic acid) Origin3 Leu (for leucine) Origin4 Pro ( for proline) Origin5 Asp (for Asparagine) Origin6 Ph (For Phenylalanine) The amino acid is the added in spots to their corresponding origins. This is done using a capillary tube.

1. Identification of amino acids by using paper chromatography

Although Aspartic Acid is considered a non-essential amino acid, it plays a paramount role in metabolism during construction of other amino acids and biochemical in the citric acid cycle. Leucine One of three branched-chain amino acids that enhance energy, increase endurance, and aid in muscle tissue recovery and repair.

2. Identification of amino acids by chromatography.

The chromatogram should then set up. The solvent at the bottom of the chromatogram should be made up of: 1 part water, 1 part glacial acetic acid and 4 parts butanol. Leave the paper in the chromatogram for 4 hours (or until the solvent has capillaried up to 5mm from the paperclip).

1. Antacid Lab. Are name brand antacids better than generic brand antacids to neutralize ...

The Conservation of Mass can be evident in the Tums mixture. The Tums mixture consisted of one gram of antacid with 30 ml of water with 2400 drops of stomach acid. Since 60 drops is equivalent to 1 ml, 40ml of stomach was used to transform the Tums solution into gastric acid.

2. Investigating the Rate of the Reaction between Bromide and Bromate Ions in Acid Solution

down foul-water sink o Methyl orange indicator, aqueous o Risks: TOXIC if swallowed o Disposal: Dilute in water to concentration of less than 0.1%, pour down foul-water sink o Water, H2O, liquid o Risks: No risk at small volumes used/produced in experiment o Bromine, Br2, aqueous o Risks: Solutions of

• Over 160,000 pieces
of student written work
• Annotated by
experienced teachers
• Ideas and feedback to
improve your own work