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In my investigation I will be investigating the effect of the Catalase on Hydrogen peroxide, I no that the higher the volume of the Catalase the faster it will react.

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Introduction

GCSE COURSEWORK Plan What is my Investigation about? In my investigation I will be investigating the effect of the Catalase on Hydrogen peroxide, I no that the higher the volume of the Catalase the faster it will react. For this reason I will be using a volume of 20 vol, from our preliminary experiment we found out that using this volume will give us the best set of results and will be easier to record the amount of gas given off. Catalase Hydrogen Peroxide Water + Oxygen H2O2 H2O + O2 What Can I Change? In my experiment there are a number of things I could change, e.g, the amount of H2O2 I use, I could use 10ml, 20ml, 30ml, 40ml and 50ml, in our preliminary experiment we found out that the more H2O2 we use the slower the reaction will happen this will help us in our experiment as we will need a medium rate of gas given off to insure our results are accurate. We Could also change the surface area of the liver to make the reaction happen at different speeds as we found out that if the exposed surface is larger then there are more enzymes exposed to the hydrogen peroxide, ...read more.

Middle

34� 37� 40� 43� and 46�C, we also know that the enzymes denature at around 45�C so we would also like to test if this theory is true by testing it at 46�C. For a table and a graph refer to attachment. Amount of Liver Solution Amount of Bubbles 5ml 150 4ml 150 3ml 150 2ml 150 1ml 100 0.5ml 45 What will I keep the same for fairness? In my experiment I will keep the following the same: * The pH, we will keep this at PH2 as the enzymes in the liver solution will work best at that ph. * Concentration of H2O2, we will keep this the same as changing this will make the experiment unfair and changing the concentration will also make our results change, to what we expected. * Amount of catalase, we will keep this the same because if we were to change it then it would effect the rate of reaction drastically, and will cause our predictions to be way off what we expect. * Amount of H2O2, we will keep this the same because if we added more H202 then it would make the reaction slower and if we added less it would speed the reaction up and this would make it very hard for us to record the results. ...read more.

Conclusion

* We will be expecting the enzymes at around 37� to be working the fastest and best as this temperature is the temperature that the enzymes work best at. * We will be expecting the enzymes at temperatures above 39�C to work slow but we will also expect the enzymes around 45�C to not be working at all this is because at 45�C and over the enzymes do no longer work they are denatured. How will I make sure that my experiment is safe? To make sure our experiment is safe we will do a number of things to insure no one is hurt, the following what we will do to insure safety. * We will wear goggles to make sure none of the hydrogen peroxide gets in our eyes as it will damage them. * We will wash our hands after the experiment as if we had hydrogen peroxide on our hands it could make its way into your mouth or eyes and cause damage. * We will not sit on any chairs/stools during the experiment as if hot liquid was spilled it would likely land on our laps. * We will use tongues to pick up hot test tubes. Jamie Stirk ...read more.

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