Introduction to Cellular Respiration in Yeast.

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QUEST LAB-Part II: Show What You Know

Introduction to Cellular Respiration in Yeast

        Cellular respiration is the process where glucose is released as energy in the form of ATP. Cellular respiration occurs in two ways. During anaerobic respiration, energy is produced without oxygen. The fermentation of yeast can occur because the yeast cells have the capability to undergo cellular respiration without the presence of oxygen. Yeast is able to break down sugar into glucose, causing the release of carbon dioxide. During the process of glycolysis, the initial two ATP molecules are what activate the glucose. Two NADH molecules are produced, causing two additional ATP molecules to be produced. Temperature is a factor that affects the rate of cellular respiration in yeast. It alters the amount of energy used as well as the amount of oxygen needed. Since yeast is a living organism, temperature is needed to activate its energy production. When too high a temperature is present, the yeast organisms die which causes the absence of carbon dioxide and energy.

Purpose:        

        To determine the differences in the rate of cellular respiration of yeast at varying temperatures.

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Hypothesis:

        If the amount of CO2 produced by yeast is related to temperature, then varying temperatures will result in different rates of cellular respiration.

Materials                                        /Apparatus:

        ∙ 18g sugar                                ∙ Graduated Cylinder

        ∙ 18g yeast                                ∙ 250 mL conical flask

        ∙ large water supply                        ∙ 1000 mL beaker

                                                ∙ stopper with glass tubing

                                                ∙ plastic hose

                                                ∙ hot plate

                                                ∙ ring stand with ring clamp

                                                ∙ balance

                                                ∙ pneumonic trough (basin)

                                                ∙ thermometer

Procedure:        

  1. A hot bath was set up by filling a 1000mL beaker with 300mL of water and placing it on ...

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