• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14
  15. 15
    15
  16. 16
    16

Investigate how effectively the enzyme amylase breaks down starch at different temperatures, and therefore to find the optimum temperature that amylase digests starch.

Extracts from this document...

Introduction

Investigating Amylase. Aim: The aim of the experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures, and therefore to find the optimum temperature that amylase digests starch. Introduction: Thousands of chemical reactions take place in our cells and those reactions need to happen quickly in order to keep us active. These chemicals are called ENZYMES. Enzymes make reactions happen at a much faster rate. Enzymes come in two main types, breakers and builders. Breaker-enzymes break down large molecules into smaller molecules. Builder enzymes join small molecules together to make large molecules. The builder enzyme does this reaction quicker. Enzymes work on substances called substrates. This reaction takes place on the surface of the enzyme which is called the Active Site. Enzymes break down large food molecules into smaller molecules, this happens inside your gut. This is called Digestion. Carbohydrates can only break down carbohydrates, to make sugars. Proteases can only break down proteins, to make amino acids. Lipases break down fats, to make fatty acids and glycerol Enzymes have five important properties: 1. They are all proteins. 2. Each enzyme controls one particular reaction. 3. They can be used again and again. 4. They are affected by temperature. 5. They are affected by Ph. The information was from 'Encarta 98' and 'Biology For You' - by Gareth Williams Background knowledge (facts): Chemicals changed by enzymes catalysed reactions are called ...read more.

Middle

For the Experiment you will need: - Stop clock - Amylase Solution - Pipette - Starch Solution - Dimple tray - Iodine solution - Heat proof mat - Gauze - Beakers - 2 test tubes - Thermometer - Tripod -Bunsen Burners Safety: The safety aspects fro this investigation it's very similar to other experiments which include boiling. Goggles are needed so that none of the chemicals used can be splashed into your eyes and damage them. Hair needs to be tied up so that it dose not catch on fire. Gloves/Tongs hot equipments needs to be handled with care and to be protect yourself from getting burnt. Bunsen burner should not be used on a blue flame when not in use. Chemicals should be used with care; any spilt chemicals should be cleaned away in the most appropriate way. (Iodine X ) Loose clothes, need to be taken of before the experiment is taken place. Fair test: To make it a fair test: * Ensure that exactly 200cm� of water is in the water bath each time the experiment is done. * Make sure that the temperature stays at 20�C, and is constant for each individual experiment. * Make sure that 1cm� of amylase is in tube B. * Exactly 5cm� of starch solution is used in test tube A * Start the stop clock as soon as test tube A is mixed with test tube B. ...read more.

Conclusion

I would have to see a strong rise in the line of best fit for there to be enough evidence to say that 60�c is the optimum temperature. In this graph it shows all the average times from both experiments. From this graph I have the information that 40�c is the optimum temperature. I am very confident that it is 40�c because from both experiments it has been 40�c that has been the optimum temperature. On the second experiment I wasn't very clear but on the rest of the experiments it was. On the first experiment that I did, it showed very clearly that 40�c was the optimum temperature. On the first experiment the line of best fit was very good and showed a clear pattern in what was happening when raising the temperature. Drawing Conclusion The results were as I predicted in my prediction. In my prediction I said that 30�c - 40�c would be the optimum temperature because the amylase would work best at body temperature. Evaluation I have found out that 40�c is the optimum temperature for amylase to digest starch. If I were to do this experiment again I would use a colorimeter because it would be more efficient and would tell me exactly how much starch was in the iodine solution. I would also expand the range of temperatures that I would use. I only went up 70�c in this experiment because I thought the optimum temperature was quite low. I was right in my prediction. Krishma Kainth 10CCH ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    We will keep the pH the same by using a buffer solution. A buffer solution will keep a solution/mixture at a certain pH, even if you add acid or alkali to the mixture.

  2. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    The activation enthalpy of a reaction is shown below. Starch and iodine solution This is a test for the presence of starch in a solution. When iodine solution is added to a solution contatining starch, a blue black colour will form.

  1. Marked by a teacher

    The effect of amylase concentration on the breakdown of starch

    3 star(s)

    The volumes needed for the concentrations I will use are shown below. (TABLE) These will be made by using the syringes to collect the amylase and water. Different syringes for different substances is important o the concentrations are accurate. They will then be put into a test tube which will be slightly shaken to make the solution mixed equally.

  2. Marked by a teacher

    Investigating the effect of temperature on the breakdown of starch by amylase.

    * Ceramic tile with depressions * Stop clock The apparatus was set up as follows: Two test tubes were taken. One was filled with 10cm3 of 0.01% amylase solution and the other with 10cm3 of 2% starch solution, measured using a measuring tube.

  1. An experiment to find of the isotonic point of root vegetables cells in contents ...

    destroy any living organism and will reduce and minimise the chance of my results being affected. This will therefore increase the accuracy and precision of my results because it will remove any living content in the equipment used and therefore it the enzyme sucrolase was present it would be destroyed

  2. Enzymes - investigate the affect of amylase concentration on starch breakdown into glucose.

    ml of water, next time use 3 ml of amylase and 2 ml of water, now use 2 ml of amylase and 3 ml of water, finally use 1 ml of amylase and add 4 ml of water. 19. Calculate the average time by adding up all your results and

  1. An Experiment to investigate the affect different temperatures have on the rate of an ...

    This could be due to bad timing or inaccuracy when reading the colorimeter. There are many ways that this experiment could be improved. Firstly, the precision when taking the first sample. On some occasions the sample may have been taken quicker than others which would explain the 3% difference in the first reading.

  2. The Effect of Starch Solution on the Activity of Amylase

    I continued removing a drop of the mixture until it failed to give a blue-black colour; I recorded this as the achromatic point. I then did the same method again but this time with the different concentrations of amylase solution that I made earlier.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work