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Investigate how enzyme activity is effected by the concentration it is working in.

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Introduction

Chemistry Coursework Aim My aim in this experiment is to investigate how enzyme activity is effected by the concentration it is working in. Prediction I predict that at higher concentrations of hydrogen peroxide the faster gas will be given off. In the potato there is an enzyme called catalyse. Enzymes make reactions happen at much faster rate. They work by breaking by braking large molecules up into smaller ones. The more molecules the greater the chance of a head on collision and a reaction. So the more molecules the quicker the reaction and the faster gas will be given off. This is a diagram of how enzymes work: The reaction of the enzyme takes place on the active site. ...read more.

Middle

If I change the temperature the enzyme will work differently if the temperature goes over 40� the active face will denature and the enzyme will not work at all. If I change the surface area there will be a greater area of enzyme to react with the hydrogen peroxide. Safety I will were goggles at all times during this experiment because hydrogen peroxide is hazardous. If I get any on my hands I will wash off straight away. Method I will full a water bath full of water. I will fill a measuring cylinder full of water and turn it upside down in the water bath making sure no water gets out. I will then make up different concentrations of hydrogen peroxide up. ...read more.

Conclusion

I will time how long it takes to collect 5m� of gas given off. I will do this 3 times and then take an average. Diagram Concentration (Vol) Time take to collect 5ml of gas in seconds 1st 2nd 3rd Average (Seconds) Rate ml/s� 20 142 146 137 141.6 0.035 17.5 159 167 162 162.6 0.030 15 182 178 192 184 0.027 12.5 195 194 204 197.6 0.025 10 225 228 233 228.6 0.022 Results Average Time taken to collect 5ml of gas in seconds Conclusion After doing this experiment I can conclude that enzymes work faster in higher concentrations of substrate. This is because with more substrate in a solution the faster the enzyme will collide with the substrate and break it down. This means that my prediction was correct. I predicted correctly because scientific knowledge I knew. ...read more.

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