Investigate how temperature affects the permeability of beetroot cell membrane.

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Permeability of beetroot membrane

Aim

To investigate how temperature affects the permeability of beetroot cell membrane.

Introduction

The purpose of a cell membrane is to control the transport of substances moving into and out of a cell. The membrane is an extremely thin layer (8 to 10 manometers (nm)) thick, which is partially permeable. It consists mostly of lipids and proteins. The lipids found in cell membranes belong to a class known as triglycerides, so called because they have one molecule of glycerol chemically linked to three molecules of fatty acids. The majority belong to one subgroup of triglycerides known as phospholipids.

Transport across membrane. Materials need to be transported into and out of cells in order for the cells to function efficiently. Cells need to take up nutrients and water, exchange respiratory gases, secrete enzymes and hormones and excrete metabolic waste products. This movement of molecules involves crossing the cell surface membrane and also the intracellular membranes of the endoplasmic reticulum and other organelles. These membranes are partially permeable and in order to have an understanding of how movement is achieved it is necessary to refer to the fluid-mosaic model of membrane structure. The fatty acid chains of the phospholipids molecules that make up the lipid bilayer are hydrophobic and so specific transport systems are required for water-soluble molecules to cross the membranes.  

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Diffusion. Diffusion is the movement of molecules or ions from a region where they are at a high conc. to a region of lower concentration. The difference is referred to as a concentration gradient.  Diffusion is a passive process, as it does not require energy. Factors that can affect diffusion are:

  • Concentration gradient
  • Size of ions or molecules
  • The distance over which the diffusion has to occur.

Beetroot membrane. In the cells of a beetroot plant, a substance called anthocyanin is contained within the plasma membrane. It is anthocyanin, which gives the beetroot its characteristic blue/purple colour. ...

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