I think that above 50 the rennin will denature this is because the rennin is a protein and proteins can not work at a temperature that is too high. (50 being too high)
This is backed up by a simulation I did on the computer to show an experiment investigating the effect of temperature on amylase. These are the results which I got:
These results back up my prediction because at 40 the amylase takes 5mins so I predict that the rennin will also work best at 40.
The plan
Apparatus
100ml beaker
2 thermometers
10ml measuring cylinder
1 pipit
2 test tubes
Stop watch
Method
I am going to use 3ml of milk and 3 ml of rennin I am going to start at a temperature of 20. I will measure the water the rennin and the milk so they are all at about 20. I will put the rennin and the milk in two test tubes and then the put these test tubes in a beaker of water the water will be at a temperature of 20 when the rennin and the milk reach the same temperature then I will mix the m together in on test tube and I will also start the stop watch once the e mixture has gone solid then I will do the experiment once more I will do the same experiment but each time I will change the temperature I will use these temperatures 20,30,40,50,60 I will do each of these experiments twice
A fair test
To make it a fair test I will use the same amount of rennin and milk each time. I will also repeat each of these experiments twice.
Safety considerations
Don’t touch the hot water with your hands
Don’t run with any thing in your hand
Theses are the results which I got:
Analysis
I found out that 40 was the fastest break point for the rennin.
My graph shows that at the beginning of my experiment as the temperature increased the time decreased but when the temperature reached 40 the time started to increase with the temperature.
My results show that 40 was the fastest break point for the rennin I think this was because enzymes work best at 37 but the rate of reaction doubles if the4 temperature increases by 10. There was no change in the milk at 60 this is because the enzyme denatures at 50 degrees it denatures because it is a protein. The enzyme was posed to denature at 50 but when I did my experiment it didn’t denature so I think that I may have done my experiment wrong. At 30 the enzyme takes along time this is because the enzyme works slower below 37.
My results nearly match my prediction in my prediction I said that 40 would be the best temperature and this was right in my prediction I also said that at 50 or over the experiment would not work but mine worked so I think I have done my experiment wrong this my be because the temperature kept dropping below 50 or it could be that it denatures at a higher temperature.
Evaluation
I think that I have succeeded in doing my experiment.
I found that it hard to keep the temperature at the right level if I put some hot water in the temperature would go too high but then if I put some ice in it would go too low this is why I think that my experiment may be wrong
