• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigate the effect of temperature on the enzyme Catalase.

Extracts from this document...

Introduction

PLANNING AIM: The aim of my experiment is to investigate the effect of temperature on the enzyme Catalase. PREDICTIONS: I Predict using my scientific knowledge that as I raise the temperature of the hydrogen peroxide, the reaction of the enzyme will get greater until it gets to 40oC where it will start to decrease. I predict this because enzymes are designed to react best at the body temperatures of the animals to which they belong. For humans this is 37oC. Catalysts are used to speed up biochemical reactions in the body. Enzymes are biological catalysts made of proteins. They have an optimum temperature and pH at which they work fastest. But they can be denatured by high temperature. The enzyme is unchanged by the reaction, and each enzyme is specific to one reaction. The "collision theory" has been developed to explain this temperature affect. The collision theory basically states for a bimolecular reaction to occur, molecules must: 1. Collide with one another to initiate a reaction. ...read more.

Middle

SAFETY: At all times there will be a teacher in the lab with me and I will be wearing safety goggles, as will everyone in the lab, at all times and will take extra precautions when handling hot water. OBTAINING EVIDENCE N.B for all the measurements of chemicals I have an error of 0.5ml. TEST 1 Temperature of Hydrogen Peroxide Time taken to reach 10ml TEST 2 Temperature of Hydrogen Peroxide Time taken to reach 10ml TEST 3 Temperature of Hydrogen Peroxide Time taken to reach 10ml AVERAGE Temperature of Hydrogen Peroxide (oC) Time taken to reach 10ml (seconds) ANALYSING EVIDENCE AND DRAWING CONCLUSIONS GRAPH: ANALYSIS: As you can see from my results from the graph, the amount of oxygen produced rises and then falls. This is because the optimum temperature is closest to 40oC so the reaction will be highest on that measurement. But as the temperature goes above that, the enzyme starts to get denatured and the reaction is not as large. When it gets to boiling point there is virtually no reaction at all. ...read more.

Conclusion

Thirdly the temperatures may not have stayed the same when taken out the water bath, as the room temperature (about 20o) would have slightly cooled them down. This would only be possible to change if I performed the experiment in the water bath which would not give enough room for other people to work. Also the bung which I was using for the experiment may not have been completely air tight so some oxygen may have escaped but that should not make a vast difference on my results. I also could have taken more results for example go up in intervals of 5oC instead of 20. I also could have measured the amount of pure oxygen as other gases may have got in but yet again I don't have the sufficient apparatus. Overall I can say with confidence that my evidence is strong enough and accurate enough to support my conclusions as all the errors would only make a hardly noticeable difference to my results. If I could make further investigation I might look at other variables such as concentration of the Hydrogen Peroxide. I also might go into researching other enzymes to compare the different reactions and temperatures in which the work. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    The prediction that I made about increasing or having a high temperature in the planning does support my conclusions that I have made here. EVALUATION The practical experiment that I did worked successfully, because what I was expecting from the experiment was that as I increases the temperature of the

  2. To investigate the effect of temperature on the enzyme Catalase.

    HYPOTHESIS I predict that the breakdown of Hydrogen Peroxide will be quicker when the temperature is increased until it exceeds 40�C. I predict that the enzymes optimum temperature is 23�C (room temperature) . This is because a rise in temperature increases the rate of most chemical reactions and a fall in temperature will slow them down.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work