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Investigating How Catalase Breaks Down Hydrogen Peroxide.

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Introduction

Investigating How Catalase Breaks Down Hydrogen Peroxide Equation: 2H2O2 2H2O + O2 Hydrogen Peroxide Water + Oxygen Introduction: There are two main reactants that affect the rate of reaction of Hydrogen Peroxide. These are temperature and concentration. I had the choice to investigate the rate of reaction using temperature or concentration. I decided to investigate the concentration of Hydrogen Peroxide being broken down by catalase. So I am going to investigate the breakdown of Hydrogen Peroxide with catalase, by adding small beads made of alginate and yeast. I will then time it to see how quickly the bead rises, in different amount of concentrations of Hydrogen Peroxide. We can see (using the equation), that Hydrogen Peroxide is broken down by catalase into water and oxygen. Hydrogen Peroxide is a colourless liquid, so we can't see when water is formed, but oxygen is a gas, so it will be seen because it will give off bubbles. Pilot Observations: When I was making the alginate beads, I found that some of them had 'tails' on them, were distorted in shape, or had air bubbles on them, which made them float. ...read more.

Middle

Variables: I made sure, that the volume of the H2O2 was added by 2.5 each time, and nearest to line as I could get it. As I stated before, the two conditions that affect the rate of H2O2 breaking down is temperature and concentration. So because we were controlling the concentration, we had to make sure that the temperature was the same throughout the whole experiment. We used a thermometer to overcome this. The dropping height we kept the same too, for the reason that I started the stop clock when I dropped the bead, which I also kept the same too. I made sure that the sizes of the beads were the same sizes. The ones that weren't, I got rid of. I expected that the temperature would mainly affect my results above all other variables. This is because the catalase works best at a certain temperature, and so I had to try and keep my beads and concentrations of liquids as constant as I can throughout the whole experiment. I used a thermometer to measure the temperature, to make sure it wasn't rising in temperature. Other variables could also influence my results, such as the concentration of light. ...read more.

Conclusion

* Thermometer Diagram of Experiment: a a Method 1: Making the bead, we mixed the yeast and the alginate together with equal proportions. i.e. I used 2ml of yeast, and 2ml of alginate. Once mixed together well with a glass rod, I then took out the rod, placed it about 10cm above a large beaker of Calcium Chloride solution, and the yeast-alginate drops drip into the liquid. When they were solidified, I repeated the process, until I had at least 40 beads. I then discarded the malformed ones. Method 2: I used a 25ml-measuring cylinder, to obtain the Hydrogen Peroxide, water and bead. Then I filled the reacting cylinder with the required amount of water and Hydrogen Peroxide to fill 20ml. I then rested the pair of tweezers holding the bead, on the top of the reacting tube. I dropped the bead, and started the stop clock at the same time. I stopped the clock, once the bead had reached the meniscus of the liquid. After recording the time, I then repeated the experiment with the next concentration of Hydrogen Peroxide and water. Analysis: Evaluation: 1 Xavier H Keenan 148 5T Biology Catalase Coursework ...read more.

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