• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating how quickly the enzyme catalase breaksdown hydrogen peroxide.

Extracts from this document...

Introduction

Biology Coursework - Planning Investigating how quickly the enzyme catalase breaks down hydrogen peroxide I will be investigating the enzyme catalase and the factors affecting the rate at which it speeds up the breakdown of hydrogen peroxide. I will be carrying out this experiment to find out if certain factors speed up the reaction at different rates. An enzyme is a substance that acts as a catalyst in living organisms, regulating the rate at which chemical reactions take place without the enzyme being altered in the process. The name enzyme was suggested in 1867 by the German physiologist, Wilhelm Kuhne (1837 - 1900), and it comes from the Greek phrase en zyme, which means "in leaven". The biological processes that occur within all living organisms are chemical reactions and most are regulated by enzymes. Without enzymes, many of these reactions would not take place at a distinguishable rate. The faster a reaction happens, the more gas it gives off and its temperature increases. Enzymes also have valuable industrial and medical uses. The fermenting of wine, leavening of bread, curdling of cheese and brewing of beer have been practiced from earliest times, but not until the 19th Century were these reactions understood to be the result of catalytic activity of enzymes. The uses of enzymes in medicine include killing disease-causing microorganisms, promoting wound healing, and diagnosing certain diseases. ...read more.

Middle

In order to understand the experiment that I am carrying out, it is necessary that I take certain factors and variables into account. There are four possible factors affecting the reaction of hydrogen peroxide that need to be noted. For a given amount of enzyme, the rate of an enzyme-controlled reaction increases with an increase in substrate concentration. At low substrate concentrations, the active sites of the enzyme molecules are not all used - there are simply not enough substrate molecules to occupy them all. As the substrate concentration is increased, more and more sites are used. A point, however, is reached when all the active sites are occupied and the amount of enzyme is the limiting factor, as the increasing of substrate concentration cannot increase the rate of reaction. If the temperature increases, it can affect the rate of an enzyme-controlled reaction in two ways: 1) As the temperature increases, the kinetic energy of the substrate and enzyme molecules increases and so they move faster. The faster these molecules move, the more often they collide with one another and therefore the rate of reaction is faster. 2) As the temperature increases, the more the atoms, which make up the enzyme molecules, vibrate. This breaks the hydrogen bonds and other forces, which hold the molecules in their precise shape. ...read more.

Conclusion

26cm3 21cm3 300 27cm3 23cm3 Gas given off 7cm3 18cm3 From my preliminary I have discovered a few corrections that need to be made in order to perfect my plan. I will also take readings from concentrations of 5% and 15% to give me a larger range from which to draw my conclusions and I will also repeat the experiment twice or three times in order to make sure my results are reliable. I will take an average from which to plot points on my graph. I didn't repeat the experiment twice for each concentration, as I did not have enough time. In my final experiment I will make sure that I have enough time to perform the experiment slowly and carefully. I will also practice starting the stop clock at the precise moment that I put the bung in place so as to get accurate results. I can now reinforce my prediction with the results that I got from my preliminary. I predict that the 5% concentration will react slower than the 10% concentration, as there will be less substrate to make use of the active sites of the enzyme. So too, the 15% concentration will react faster than the 10% concentration but slower than the 20% concentration as it has more substrate than the 10% concentration, but less substrate than the 20% concentration to fill the active sites of the enzyme. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    How does the concentration of enzymes affect the breakdown of starch by a-amylase in ...

    4 star(s)

    Notice that there is another anomalous result in experiment 2, for the 4% concentration. This will probably have been caused by the same reason as the other anomalous results, explained above. All anomalous results obtained in these experiments will be ignored in the final average of the results.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    * Because of the restricted number of water baths our experiment could not be carried out in one, and I had to use the acclimatisation process in order to be able to carry out the reaction at the desired temperature.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Rearrange and replace the boiling tubes as the experiment continues, so that they are close to you to reduce the risk of solution being lost as drips when transferring it from one place to another (however unlikely). 8. Record the temperature of the distilled water, making sure that the beaker

  2. Reaction of Catalase and Hydrogen Peroxide

    This I will be doing by using the cork borer, knife and ruler to make sure that all the potato pieces are of equal length and width. This is because if there is any change in the surface area of the potato than it will have an effect on the number of enzyme molecules present in the solution.

  1. This is an experiment to show how different concentration of celery tissue enzyme, catalase ...

    there are few free enzyme molecules, so adding more substrate doesn't make much difference Inhibitors: - Inhibitors are substances, which alter the enzymes activation. There are two main types of inhibitors: * Competitive inhibitor * Non-competitive inhibitors Competitive inhibitors: - Competitive inhibitors have the same shape as substrate and therefore

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    sites are available and hence more substrates can react at any one time therefore increasing the rate of reaction. The graph below shows how rate of reaction alters with increasing concentration, the direct proportionality of the two factors is shown by the straight line intersecting the origin.

  1. Investigate how changing the concentration of hydrogen peroxide (substrate) affects the rate of reaction ...

    They do not react with the site and leave after a time without a product forming. The rate of reaction is increased when these are present because when the inhibitor is in the active sight no substrate can enter the sight.

  2. The Application of Enzymes in Industry and Medicine.

    Within industry enzymes are also widely used, even more so than within medicine. The study of industrial enzymes and their uses is enzyme technology. The detergent, food and starch processing industries still account for 75% of the 'bulk' enzyme use.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work