• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the affect of Copper Sulphate on Liver.

Extracts from this document...


Title: Inhibitory of copper Sulphate on enzyme catalase found in Liver Aims: Investigating the affect of Copper Sulphate on Liver. Hypothesis: Catalase is a red crystalline enzyme that consists of a protein complex with a haem groups in the centre. Catalase catalyses the decomposition of hydrogen peroxide into water and oxygen: 2H2O2 2H2O + O2 An enzyme inhibitor is a substance that slows down the rate at which an enzyme-catalysed reaction takes place. Many enzyme inhibitors work by binding with the enzyme, with the result that the enzyme can no longer bind with its substrate Some inhibitors have shapes rather like the enzyme's normal substrate molecule, allowing them to bind at the active site of the enzyme. They are called active site-directed inhibitors. If there is an inhibitor molecule in the active site, the substrate cannot bind there. Some active site-directed inhibitors bind permanently to the active site, so that they permanently inactivate the enzyme. While the inhibitor is out of the active site, it is possible for a substrate molecule to slot in, so the inhibitor will not completely stop the reaction, just slow it down. The inhibitor and the substrate are competing for the active site, so these inhibitors are sometimes called competitive inhibitors. The chances of a substrate molecule getting into the active site depend on: > The relative concentrations of substrate and inhibitor - the more substrate molecules there are compared to inhibitor molecules; the more likely it is that a substrate rather than an inhibitor will bind with the enzyme's active site. > How long the inhibitor stays attached to the enzyme before moving away - the shorter the inhibitor-enzyme complex lasts, the more chance the substrate has to bind with the active site, and the less the inhibition of the reaction. ...read more.


Quickly connect the rubber bung, which is connected to the tubing, to the test tube. XII. From the Syringe measure the gas given off, record it in a table XIII. Repeat the first 12 steps, 6 times each time changing step 5 to 0.3,05,07,09 and 1 mole. To get 0.3 mole add 3cm3 of copper sulphate with 7cm3 of distilled Water and so on. XIV. Repeat step 1 to 13 at least three times (time dependent) so that an average can be obtained. Repeating the experiments several times will help to produce better and more accurate results, as any inaccuracies in one experiment should be compensated for by the other experiments. XV. Create a table of the results, from these results, a graph can be plotted with concentration on the x-axis and the volume of gas released on the y-axis. Preliminary Measurements: In my preliminary work I found that as you increase the concentration of hydrogen peroxide the rate of reaction also increase this is because there is more hydrogen peroxide molecule to combine with the enzyme. In this experiment the hydrogen peroxide stays constant, the reason why I choose 5 cm3 of hydrogen peroxide is because 10 cm3 of H202 would be too much and we did not have enough liver as well so therefore 5 cm3 of H202 was reasonable amount of H202. Also the mass of the liver in my experiment stays constant, I decided to choose 1Kg; the reason for this is because in my preliminary work I found out that the more the mass of liver the greater the enzyme present and if there are more of enzyme present; then the reaction would take quite long time. ...read more.


> Repeat the experiment more times There are numbers of other further experiment we could do: > Do the same experiment but in the end add more hydrogen peroxide to see how much amount of oxygen is given off, if more then last reading given off that means that the inhibitor is competitive reversible. > We could find out where in enzyme does copper sulphate combine with the enzyme does it combine at the active site or elsewhere on the enzyme. > The temperature remained constant at room temperature we could have changed the temperature see what exactly what happens To find percentage error: I measured the movement of the syringe to 3 significant figures. So the range can be + 0.05 or -0.05 the results. To find the percentage error we add the result or minus it by {(0.05/the result)and timing it by 100}. This is going to give us a positive percentage error and negative percentage error. Experiment Average(cm) Percentage error(%) PE + Average PE-Average 1 14.80 15.13 29.93 0.33 2 11.50 11.92 23.42 0.42 3 8.60 9.16 17.76 0.56 4 7.40 8.11 15.51 0.71 5 1.60 4.10 5.70 2.50 Key PE- percentage error The range between pink and yellow line shows where the results could lie, it shows the maximum and minimum point of the results. If you analyse the graph we can see that the line of best fit for the average is slightly in the middle of positive and negative percentage error line of best fit. Which means that the results are accurate as percentage error is not that great. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    Time taken for the solution to drop 1.5 arbitrary units of light absorbance/s 1 2 3 Average 0 160 190 182 177 0.1 422 452 465 450 0.2 684 673 670 676 0.4 804 830 822 819 0.6 884 910 879 891 0.8 1401 1428 1389 1406 1.0 942 910

  2. Marked by a teacher

    Investigate the effect of copper sulphate on pepsin activity.

    4 star(s)

    > Albumen concentration must remain constant. This is because if the concentration of albumen is increased for one experiment, the rate of reaction in that experiment will increase, as there are more substrate molecules to form enzyme-substrate complexes, and similarly, if the concentration is lowered, the rate of reaction will decrease.

  1. The Effect Of Copper Sulphate On Pepsin Activity.

    Wash thoroughly with running water. Get medical advice if irritation develops. If split on to skin: Not expected to require first aid measures. Wash exposed area with soap and water. Get medical advice if irritation develops Disposal Copper sulphate: Very toxic to environment, dissolve salts in 10 litres of water and wash down drains.

  2. Does ethanol causes greater inhibition of pig liver catalase than of yeast catalase

    If it is to strong my catalase beads will float as soon as they are dropped in to the hydrogen peroxide. But if the concentration is to weak the beads will take to long to reach the surface. I would like the beads to reach the bottom of the beaker and start to rise again soon after that.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    This resemblance allows the inhibitor to easily bind to the enzyme's active site, making it impossible for the substrate to bind to the active site. Very common chemical inhibitors are solutions, which contain metal ions, such as the one I am using: copper sulphate.

  2. Exploring the Effects of Copper Sulphate as an Inhibitor on the Enzyme Amylase

    From there it will be a regulated boiling water bath to allow the Reducing Sugar experiment to react. 6. Spotter Tray - to place the iodine in and amylase the results. 7. Iodine- to show the levels of starch present. 8. 18 boiling tubes for each of the different processes.

  1. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    or copper sulphate substrates in the contents. As a result of more particles, the rate of the reaction will increase, as there is a higher chance of a collision. This must also be kept constant for each reading I am to take The other factors outlined above will also affect the way the reaction goes in similar ways.

  2. Effects of Copper Sulphate on the Activity of Catalase

    Catalase contains Fe in its active site. Cu++ may inhibit catalase by displacing the Fe from the enzyme. If the Cu is removed and Fe added back, activity is likely to be restored. This suggests that Cu is a non-competitive inhibitor and it alters the enzyme such that it no longer functions.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work