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# Investigating the breakdown of Hydro Peroxide (H2O2) with the enzyme catalase.

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Introduction

Nicholas Cuthbert Science Coursework 17/09/01 Investigating the breakdown of Hydro Peroxide (H2O2) with the enzyme catalase. AIM: In my experiment I am going to investigate the breakdown of hydro peroxide (H2O2) with the enzyme catalase, which is found in Liver. PLAN: I am going to put a piece of liver in to a 250 cm3 measuring cylinder and add 10cm3 of 20% hydro peroxide and measure how much gas is collected in the measuring cylinder. I will repeat that method for 40% 60% 80% and 100% and put the results in a table I will then chop up a piece liver and place it in a 250 cm3 measuring cylinder, I will then add 10 cm3 of 20% hydro peroxide and measure the gas given off. ...read more.

Middle

The chopped liver mixed with 100% hydro peroxide will produce the most gas. EQUIPMENT: 1 liver, 2 hydro peroxide 20%, 40%, 60%, 80%, 100%'s, 3 250ml measuring cylinder, 4 stirring rod, 5 50ml measuring cylinder, 6 scissors, 7 tweezers, 8 paper towels, 9 container for liver. FAIR TEST: It is important to make an experiment a fair test so that the outcome of test is right and fair in this experiment I need to: 1. Repeat the test. 2. Keep the amount (volume) of H2O2 the same every time I do a test. 3. Keep the mass of the liver the same. 4. Chop the liver up into quarters every time. VARIABLES: Variables are things that change in your experiment and in my experiment: 1. ...read more.

Conclusion

94 130 173 275 40 80 130 180 270 40 80 110 163 250 AVERAGE: 37 80.8 122.2 170 251.3 PARTICLE THEORY: 20% 0 0 0 0 40% 0 0 0 0 0 0 60% 0 0 0 0 0 0 0 0 80% 0 0 0 0 0 00 0 00 100% 0 0 0 000 0 000 0 0 EVALUATION: My experiment went well and I made it a fair test and my prediction matched my results I said that the chopped liver would produce more gas and it did. The chopped liver mixed with 100% hydro peroxide produced the most liver, which my prediction states. My results table shows a few anomalous results but my personal results where good. We could produce more gas by chopping the piece of liver into more pieces and adding more cm3 of 100% hydro peroxide. ...read more.

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