Investigating the breakdown of Hydro Peroxide (H2O2) with the enzyme catalase.

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Nicholas Cuthbert                Science Coursework                17/09/01

   

Investigating the breakdown of Hydro Peroxide (H2O2) with the enzyme catalase.

AIM:        In my experiment I am going to investigate the breakdown of hydro peroxide (H2O2) with the enzyme catalase, which is found in Liver.

PLAN: I am going to put a piece of liver in to a 250 cm3 measuring cylinder and add 10cm3 of 20% hydro peroxide and measure how much gas is collected in the measuring cylinder. I will repeat that method for 40% 60% 80% and 100% and put the results in a table I will then chop up a piece liver and place it in a 250 cm3 measuring cylinder, I will then add 10 cm3 of 20% hydro peroxide and measure the gas given off. I will repeat that test for 40% 60% 80% and 100%.

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ABOUT H2O2: Hydro peroxide is an unstable chemical compound, H2O2 used as an oxidizing agent and bleaching agent an antiseptic and rocket fuel. We are using H2O2 in our experiment by pouring five different H2O2’s 20% 40% 60% 80% 100% one at a time in too a cylinder which there is a liver.

         

    HYDRO PEROXIDE > WATER+OXYGEN

                           H2O2 > H2O   + O2

PREDICTION: I predict that the chopped liver will produce more gas than the whole liver because the chopped liver has a larger ...

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