• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the effect of one variable on the rate of an enzyme reaction.

Extracts from this document...

Introduction

Investigating the effect of one variable on the rate of an enzyme reaction. Aim The aim of the experiment is to find out how the level of hydrogen peroxide which is the substicute for catalose, effects the rate of reaction when mixed with 5cm3 of yeast. So my aim is going to be, to measure the amount of oxygen given off when the two react, to come to a conclusion of what effect does the amount of hydrogen peroxide have on the yeast (catalase). Prediction Hydrogen Peroxide catalase water + oxygen 2H2o2 2H20 O2 This is the reaction that is being investigated to find out how the concentration of hydrogen peroxide affects the rate of reaction if at all. In this reaction between hydrogen peroxide and the catalase the compounds of water (2H2o) and oxygen (02) are given off, which I will measure to come to this conclusion. The way hydrogen peroxide and the catalase react is through the collision theory, which will now be explained. The yeast cell has many enzymes but it is only the catalase inside the yeast, which is capable of breaking down the hydrogen peroxide, this is because this enzyme is specific for hydrogen peroxide. ...read more.

Middle

For the experiment to work you must firstly pour water into the 100cm3 cylinder and turn it upside down ready to lose oxygen if my prediction is correct. Then you must pour 15cm3 of 100% H202 into the test tube, and as soon as 5cm3 of yeast is added and the lid is put on the timer will be started. Then every 30 seconds a result will be taken of how much oxygen has been lost from the measuring cylinder, this happens as the oxygen pushes out the water from the reaction between the catalyse and the hydrogen peroxide. This will then complete the experiment after 180 seconds are gone and the results can be examined to be out into a results chart graph, and a conclusion to this. Results Analysing the evidence The results show that any given variable of Hydrogen Peroxide will make the enzyme react. And the more concentration of hydrogen peroxide the faster rate of oxygen is given off in a chemical reaction. The trend that I can see in my results shows that with the concentration of acid at its strongest, the oxygen is given off at a rate, which is fast, but not a consistent amount each 30 seconds. ...read more.

Conclusion

Overall though the experiment has been very successful with the question of how does one variable affect an enzyme reaction being answered, with the answer of it reacting with H202 to give off oxygen and water. And further work shows that the higher the concentration of H202 the faster the amount that oxygen is given off to end this investigation. Apparatus The equipment needed for this experiment is. X 1 100cm3 cylinder (measure) X 1 a Bunsen burner tubing X 1 water bath X 1 test tube Method Set up the experiment as shown in the diagram. For the experiment to work us must firstly pour water into the 100cm3 cylinder and turn it upside down ready to lose oxygen if my prediction is correct. Then you must pour 15cm3 of 100% H2O2 into the test tube, and as soon as 5cm3 of yeast is added and lid is put on the timer will then be started. Then every 30 seconds a result will be taken of how much oxygen has been lost from the measuring cylinder, this happens as the oxygen pushes out the water from the reaction between the catalyse and the hydrogen peroxide. This will then complete the experiment after 180 seconds are gone and the results can be examined to be put into result charts graphs and an conclusion to this ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. In this experiment I will be investigating and analyzing the factors affecting the rate ...

    At this stage it is said to be denatured. When the enzyme is denatured we expect it not to work at all. This might seem odd as it did work at 70ºC. Well there is an explanation to that. It could have been because the hydrogen peroxide was reaction with impurities within the test tube and caused bubbles to push the bead up.

  2. Factors That Affect the Rate of an Enzyme Reaction.

    I have decided to measure the rate by following the volume of gas produced by collecting the gas in a burette and timing the taken to collect 30cm3 of gas. The rate can then be worked out in the following way: Rate=30/Average Time This gives the rate in cm3 of

  1. Hydrogen Peroxide and catalyse.

    The VM value for hydrogen peroxide is 1012 molecules of oxygen per molecule of catalase per second. The KM value in this case is about 5E-8. Velocity =VM (S) KM+(S) I found the value of (S) by calculating the amount of moles per litre using the relative molecular mass of water and hydrogen peroxide and the Avogadro constant.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    My method and the 'Agreed Method' had many differences, some were positive whilst others were negative. I used concentrations of 100%, 80%, 60%, 40%, 20% and the mandatory 0%, but the Agreed Method dictated that we used variations with the factor of 25.

  1. Catalyse Investigation

    Temperature - As temperature increases, molecules move faster (kinetic theory). In an enzyme catalysed reaction, such as the digestion of sucrose into glucose and fructose, this increases the rate at which the enzyme and substrate molecules meet and therefore the rate at which the products are formed.

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    Although percentage celery extract is being varied with each set of experiments, a constant volume of celery extract/enzyme is kept so that the net volume of the test solution is kept constant. Substrate concentration - this is also a controlled variable and is kept constant throughout the experiments.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work