If there is a larger concentration of sugar/glucose than water molecules, the water inside the chip will be “sucked,” out of the chip decreasing it in SIZE and making the chip become flaccid. The water molecules have been “sucked,” out of the chip by the sucrose solution this makes the chip burn the flaccid.
So basically I think that when there is a low concentration of sugar molecules and high concentration of water the chip length will increase. This will happen because the water will want to diffuse by osmosis in the chip and as it is a smaller molecule it can enter the chip were as sucrose can not.
Were as when there is a large concentration of glucose solution the water molecule’s inside the chip will want to escape out to balance it.
My preliminary work supports my views on osmosis because it shows when there is a large concentration of sucrose the mass decreases.
Preliminary Work
My preliminary work has been done on the on a special programme on the intranet on a special programme designed for preliminary work. In order for me to be to do a fair test I need to consider what variables I am going to use to produce a good and fair test. To help me decide these variables I used this computer software.
The variables are the solution volume, the length of the experiment, temperature and the variable surface area of plant tissue. By doing my preliminary work I was able to work out the variables and how to improve my experiment. The one thing I learnt from my experiment is that I am going to need to repeat my experiment more than once. Also I will have to at least spend an hour on my experiment. I have chosen from my preliminary that I will use 7cms for my potato because it will give me the best results as it has a larger surface area, therefore more osmosis taking place and it is easier to measure more accurately than maybe 3cm. The only problem with the computer is the results is not that accurate so for my final experiment I will do the experiment myself.
TABLE TO SHOW PREMINLARY COMPUTER DATA- SUCROSE CONCENTRATION- CHANGE IN MASS.
Method
Apparatus: Glucose solution, Distilled Water, 1 potato, cork borer, 7 test tubes, 1 kitchen knife, 2 100 cl measuring cylinders, a marker pen, stop watch, a tile and a ruler
- I will take one potato and take a kitchen knife to peel the skin to make it a more fair experiment.
- Using a cork borer to cut sections out of the potato (so same surface area) into circular tubes and used a knife to cut each one approximately to 7cm in length. Record the length of potato. The cork borer is sharp so we need to be careful while cutting potato.
- I then place 6 test tubes and label it 1-6 on the test tubes putting them into the test tube rack.
- Using a measuring cylinder I measure out different amounts of glucose solutions and also distilled water by using a different measuring cylinder (fair test) mixing it into different amounts glucose solutions each time. Then pour into the test tubes.
- I will put one potato in each test tube with the different amount of solutions and start my stopwatch.
- After 1 hour, I will measure the length of potato by using a ruler and then the length will be recorded.
- I will repeat the experiment exactly the same and it will give me total of two sets of results giving me an average of the two results.
- Reasons For Method
- I am using 7cm for my potato because from my preliminary work it shows me that I should use a longer piece potato, because it has larger surface area so increasing osmosis.
- I am recording 6 results because I need to plot an accurate pattern for my graph in my obtaining evidence.
- Surface area must be cut accurately so the same size and water concentration must be according to the table above to get more accurate results.
- There should only be one potato used for the whole experiment to give a fair test and in case potato’s differ in concentration.
- Use a thermometer to measure room heat to check fairness.
- Time of osmosis must be the same to give a fair test
- The potato cuttings must be completely submerged in solution so osmosis can take lace fairly.
- Potato volume should cover potato to give fair test.
- Use clean equipment for accuracy and fair testing.
- Safety Considerations
- Be aware of the Sharpe knife and the end of the cork borer because often there is a risk of being cut.
- Also be shore to wear goggles from protection of the glucose because even know it is only glucose if you get it in your eye it is shore to harm your eye
- I will a lab coat because it will save me getting potato on my top maybe staining it.
Results for Main exp on Osmosis
Experiment 1
Experiment 2
Average
This graph shows me the average % change between the 2 experiments I have done.
Analysis for the osmosis experiment
From this experiment I have found out that when sucrose concentration increases so the potato gets smaller. So my results agree with my hypothesis and my graph agrees with my predicted graph.
The patterns in my on my graph are as the Sucrose concentration increases so there is more diffusion making the potato decrease in size. When the Sucrose concentration is smaller there is a decrease in diffusion making the potato larger.
Conclusion
My results agree with my background knowledge and my hypothesis because it shows me in my results that when concentration level is high the diffusion occurs from potato to solution so potato loses water and becomes smaller and when the concentration level is low water diffuse from solution to potato gains water and therefore becomes bigger.
My results agree with my hypothesis because it shows me osmosis is the diffusion of water molecules from one side of high water potential to low water potential through a partially permeable membrane. When there is a low concentration of sucrose the water diffuses into the cytoplasm and through the selectively permeable membrane and my results show that this is true because the cytoplasm increases when a low % of sucrose. When osmosis takes place there is a high concentration of sucrose therefore the water from the potato cell diffuses out of the cytoplasm and vacuole through the selectively permeable cell membrane. My results prove this because when there is a high concentration of sucrose there is a decrease of size in the potato.
Pictures of Osmosis and plant cells.
Evaluation of Experiment.
The procedure I used for this was experiment was good because it was quite simple and fair. I could have extended my experiment to make it more fair and accurate by weighing the potato as well as measuring it to make it fairer experiment.
I would have weighed the potato before and after the experiment to see the change in weight during osmosis. This could have been one thing that I could have done to make the experiment more fair and accurate.
During my experiment I felt my experiment went very well because I got no anomalous readings. My results were impressive because they agreed with my prediction and I managed to get a very good range of results on my graph.
My method was good and fair because gave me good results and a fair test but I believe I could have improved it by:
- Use a water bath because it would have kept the temperature the same to give better and more accurate results
- Clean all the apparatus used to make it a fairer test.
- Use a wider range of concentration levels to get more accurate readings and more of a curve n my graph.
- I maybe could have used different solutions, which would have made the results of the experiment more accurate.
- I could have used a different tester than potato maybe a plant of some type as from a plant easier to use the same plant for every experiment.
Overall my experiment was successful because it agreed with my hypothesis which was “ the higher the concentration level the smaller the potato chip will become the lower the concentration level the bigger the potato will become”. I also have completed my aim and have got some good results from the experiment.