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Investigating the effect of pH on the activity of phosphotase enzymes

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Biology coursework: Investigating the effect of pH on the activity of phosphotase enzymes Background information Enzymes are biological catalysts, which speed up reactions. They have specific active sites, and are not used up, but (in theory) can be used over and over again. They have optimum working temperatures, and likewise, an optimum working pH. The point of this experiment is to find out the optimum working pH of the phosphotase enzyme. The word equation to a reaction involving a phosphotase enzyme would be: Enzyme + Substrate E/S Product + Enzyme The rate of reaction of an enzyme-catalysed reaction depends on 2 things: 1. How fast substrate is being used up; 2. How fast product is being formed The phosphotase enzyme occurs in both animal and plant tissue. If catalysed, the breaking of phosphotase bonds in organic phosphotase leads to formation of phosphates. 0. Organic phosphotase Phosphate + organic residue Phosphotase enzyme Substrate product This reaction releases phosphate ions into the metabolic pool. Phosphate is an essential component of biological molecules. The phosphate is found in DNA, RNA, ATP and phospholipids (in cell membranes). Phosphotase is also associated with allergens in pollen grains. Important graphs Graph 1 Steeper side Optimum temperature Graph 2 The optimum pH point depends on which pH the enzyme is used to ...read more.


Of the acidic-based enzymes, I expect the enzymes in the pH closest to its optimum, even if this is not the strongest acidic pH. Therefore if the optimum pH of the phosphotase enzyme is pH 6, then I expect the enzymes in the closest range of PH 6 to have reacted most, sop if there were enzymes in a buffer solution of pH1, and there were also enzymes in a pH of 4, then I would expect the enzymes in pH 4 to have reacted more. However, if the enzyme were to deform at pH 3, then I expect the results to be the same for when the pH of the buffer is 1, or 2. Preliminary Results Magenta reading (m) pH Time (minutes) 0 3 15 0.3 4 15 0.4 5 15 0.2 7 15 0 9.2 15 This is just one set of results which I used as a guidance for all the rest of the results that I was about to obtain. From this experiment, I decided that 15 minutes was enough in order to obtain a good result, and also that the centrifuge only needed 3 minutes to obtain good results. Results Due to a lack of time, I only got 1 extra set of results, but in order to obtain a good set of results and so form a good graph, I compared my results to that of a friends. ...read more.


Because I only have 3 results to find an average of, there is the greater probability that my 1st set of results was wrong, but there is also the probability that my 2nd AND preliminary results were wrong, where my 1st set of results may have been closer to the actual optimum. Overall, I think my results came out well, because there was only a slight variance in all 3 results, with no really obvious anomalous results, but if there were to be one, I would think that it would be the pH 4 result of my 1st set of results, because the other 2 had exactly the same results but this one was slightly different, and gave the whole graph a greater peak. In order to extend this project, I could have given more time for the enzymes to work in the water baths, and then, because they are all left in the water for the same time, the results should have come out directly proportional. Then, I could have tried to use different enzymes with an alkali pH, and see if the graph turns out similar, and then more enzymes with different pH's, and then different enzymes with the same optimum pH. ?? ?? ?? ?? By shin young Kim 5 aleph ...read more.

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