Investigating the effect of sodium chloride on the action of antibiotics on bacteria growth

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Investigating the effect of sodium chloride on the action of antibiotics on bacteria growth

Abstract

Effect of sodium chloride on the action of antibiotics on bacteria growth was investigated using Escherichia coli with antibiotic penicillin. Sodium chloride delayed the size of area of bacteria growth with increasing concentration of sodium chloride for incubations at 25ºC. from statistical and graphical analysis growth inhibition indicates that antimicrobial activity is correlated with the concentration of sodium chloride. This was proven to be the case with a null hypothesis testing at 5% significance level was rejected.

Aim

To investigate how varying concentration of sodium chloride affect the action of antibiotic on bacteria growth by measuring the area of bacteria growth inhibition around the antibiotic discs on an agar plate.

Rationale

Cystic fibrosis is one of the most commonly inherited diseases in Europe affecting at least 1 in 20 persons. [1]"It is an autosomal recessive condition whereby two alleles carrying a polymorphism in the cystic fibrosis transmembrane conductance regulator (CFTR) gene" fails to function properly due to mutation in the gene.

Fig 1 shows the structure of cystic fibrosis transmembrane regulator (CFTR)

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