• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the effect of substrate concentration on the activity of catalase enzyme.

Extracts from this document...

Introduction

Investigating the effect of substrate concentration on the activity of catalase enzyme Introduction Catalase is a catalytic enzyme that speeds up cellular reactions within our body making it a biological enzyme. It is used to turn the potentially harmful effects of hydrogen peroxide into water and oxygen. In this experiment I will be testing the reactions of different concentrations of hydrogen peroxide with the enzyme, yeast. Word and Chemical Equation: Catalase Hydrogen Peroxide Water+Oxygen H O H O+O Catalase works like a lock and key system when breaking down the hydrogen peroxide as shown and explained below. Lock and key hypothesis The factors that can effect enzyme activity are: Concentration of Catalase- the higher the concentration of catalase the greater the chance the hydrogen peroxide has in colliding into the catalase molecules and reacting with them. Concentration of hydrogen peroxide-the higher the concentration of hydrogen peroxide the greater chance there is in the catalase enzyme colliding into the hydrogen peroxide molecules and reacting with them. PH-The active site of an enzyme can be changed by very acid or very alkaline conditions. When acid reacts with an enzyme it changes the shape of the active site making the "Lock and Key" theory hard to perform. Temperature-particles have more kinetic energy when they are heated this means they have more energy to collide with other particles making them react quicker. ...read more.

Middle

because if one has more time than another then there is a high chance that the oxygen gas would have more time to be released. * Finally, I will change the water in the plastic tub after every experiment as the heat caused by the reaction will heat up the water in the tub causing an intervention in the temperature of the test tube. Which in turn could speed up the reaction between the hydrogen peroxide and yeast, interfering with my results. To ensure my results are reliable I will test the same percentage of hydrogen peroxide three times then take an average from my results. As hydrogen peroxide is a powerful oxidising agent and can be potentially damaging to cells, I will wear safety glasses and work safely and sensibly throughout the experiment. After I have completed the experiment I will use my data to produce graphs to see if there is any correlations or patterns according to the amount of gas released and the percentage of hydrogen peroxide in my results. Prediction I predict that as the concentration of the substrate increases, the rate of reaction will speed up because the more particles there are the higher the chance of collision. When the substrate and catalase particles collide with each other they form enzyme substrate complexes this then breaks down the hydrogen peroxide into water and oxygen. So as the concentration increases more enzymes substrate complexes are formed but at a faster rate. ...read more.

Conclusion

As a result of this, I can prove why I repeated the tests for each of the concentrations, without doing so my results could be unreliable as they would show errors in my experimenting and not in the actual result of the reaction. Evaluation I think the quality of my results are fairly accurate but considerable changes should happen if I was to act upon this experiment again this is so that I can be sure my results are a hundred percent reliable. For instance, I should have checked the room temperature before each test so that there weren't any fluctuations and made sure the test tube with the hydrogen peroxide and yeast in was at the same temperature for each of the tests. I could also have done five tests instead of three to have made my results even more accurate then recorded them precisely up to the nearest millimetre cubed. I could also do some further work to add additional relevant evidence to this experiment. One way I thought of was to heat the test tubes the hydrogen peroxide and yeast are placed in to get an increased reaction. Another way would be change the concentrations of the hydrogen peroxide from going up in twenties to going up in fives to increase the accuracy of my results. Concluding this experiment I would say that concentration in a reaction is extremely important in what result is wanted or to be tested. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The effects of Hydrogen Peroxide concentration on the activity of Potato Catalase

    4 star(s)

    substrate to bind with, so this means that the reaction will be limited by substrate concentration. From this, I predict that as the concentration of Hydrogen Peroxide increases, the activity will initially increase, and the rate of increase slowly begin to level off until the rate of increase becomes zero.

  2. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    increases until the catalase enzyme is denatured and the rate of reaction slows down. For both the immobilised and non-immobilised catalase enzyme, the increase is steady until it reaches its peak. Then for both immobilised and non-immobilised catalase enzymes there is a dramatic fall as the enzymes start to become denatured.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Gently shake the boiling tube to get rid of any bubbles. 12. Sticky label a 5 cm3 syringe 'H2O2'. 13. Record the temperature of the H2O2 in the beaker. 14. Immediately after, take the 'H2O2' syringe and use it to collect 5 cm3 of hydrogen peroxide using the same technique as outlined in step 7.

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    the extent of any anomalies on a graph where the line of best fit must be drawn. This would mean that the graph drawn could be more complete, and better results would be obtained. I would only have been able to test 9cm3 of the catalase solution with 1cm3 of

  1. Reaction of Catalase and Hydrogen Peroxide

    Getting over with this I will take 30% concentrated solution of hydrogen peroxide from the bottle. I will also be taking 100ml of hydrogen peroxide using the measuring cylinder. Then I will pour the hydrogen peroxide from the measuring cylinder into the beaker containing water.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    Low Temperature High Temperature Behind all this theory is the Lock and Key theory. A lock and key theory is used to explain the way an enzyme and a substrate work. Enzymes and substrate have the same similarities of how a lock and key works.

  1. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    The optimum temperature differs from enzyme to enzyme. Some work efficiently at high temperatures while others at low. "Saccharomyces cerevisiae are active in a very broad temperature range - from 0 to 50� C, with an optimum temperature range of 20� to 30� C."

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    It is important to specify that the enzyme does not form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape. Because the enzyme does not form chemical bonds with the substrate, it remains unchanged.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work