• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of Hydrogen Peroxide By Catalase In Immobilised Yeast

Extracts from this document...


M1-Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of Hydrogen Peroxide By Catalase In Immobilised Yeast Problem: Hydrogen peroxide is a waste product of the metabolism (chemical reactions of living cells). It is a powerful oxidising agent, which would damage living cells unless it was destroyed. Hydrogen peroxide will eventually decompose into un-harmful substances such as water and oxygen however in this slow process. 2H O 2H O + O However the human body has a process which speeds up the breakdown of hydrogen peroxide. The human body produces an enzyme to speed up the breakdown of hydrogen peroxide; this enzyme is called catalase. Hypothesis: In my investigation I aim to explore one of the factors that would affect the catalysis of an enzyme; my research from textbooks, CD-ROMs (Encarta) and the Internet (www.britannica.com) tells me that factors such as; Temperature, pH, Substrate Concentration, Inhibition, Enzymes Cofactors and Enzyme concentration would greatly vary the rate of enzyme catalysis. However from this research I have decided substrate concentration (hydrogen peroxide) is the factor which would able my experiment to yield good conclusions. For this reason I have decided to look at how a change in temperature would affect the rate of a reactions. ...read more.


concentrations. Step 1: Prepare the alginate beads by mixing a solution of 3cm of yeast, 3cm of sodium alginate and 3cm of air. The yeast in this solution is the source of the catalase. I have decided to use the alginate as my source for the enzyme-in this way the enzyme is immobilised. I have decided to test these alginate balls with 11 varying concentrations of hydrogen peroxide and I will also test these 11 concentrations 5 times and take an average. Because of this I will make around 40 alginate balls providing that all these balls are evenly produced. In this stage I will wear goggles and if I spill some of the hydrogen peroxide concentration on my hands I will immediately wash them because hydrogen peroxide is highly corrosive. Step 2: Now I will test these alginate beads with each varying concentration of hydrogen peroxide. As an example, having picked an evenly prepared alginate ball I will drop it into the test tube containing a hydrogen peroxide concentration. With a stopwatch I will time from when I place the alginate bead into the concentration and time that it takes until the bead rises to float on the top of the hydrogen peroxide concentration. When I am timing the reaction I will have to be very responsive to keep my investigation accurate. ...read more.


In my experiment I used the same hydrogen peroxide concentrations for each of the 5 tests simply because there weren't enough test tubes and concentrations for the whole of the class to use. So in each test it could of meant that there would have been less and less hydrogen peroxide molecules in the concentration for the enzyme to react with ultimately meaning that the rate of reaction would have become slower and slower in each test. If I were to extend this investigation I would definitely use a different hydrogen peroxide concentration each time. I think that because I have measured the enzyme decomposition five times and have taken an average from this I feel my results are very reliable, the fact that I had repeated it 5 times means that any mistakes I made I could have looked at, rendered the mistake and repeated the test. Within the limits of how accurate I could keep my experiment I was very rigorous and because of this I feel I have managed to yield evidence to support the prediction that- if there are sufficient enzyme molecules, an increase in the substrate concentration, will produce an increase in the rate of reaction To improve my results I could start by testing the enzyme decomposition with a wider range of hydrogen peroxide concentrations as this would better my timings for the average rate of reactions. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The Effect Of Temperature on the Respiration Of Yeast.

    5 star(s)

    of water is below the level of TTC-yeast mixture and in another test the level of water it is over then this will effect the time taken for the TTC-yeast mixture to change its temperature and this will effect the time taken for the TTC solution to change colour from

  2. Marked by a teacher

    The effect of temperature on the enzyme Catalase.

    4 star(s)

    Prediction - By using the range of temperatures that I am (15�c-50�c), I think that the Catalase will work best and produce the most froth at potato temperature, which is about 15�C. I think this because in our theory I learned that over 40�C the Catalase enzyme breaks and denatures,

  1. Investigating the effect of temperature on the activity of free and immobilised enzymes.

    * Enzyme on the skin or inhaled should be washed with plenty of water. * Liquid preparations are inherently safer but it is important that any spilt enzyme is not allowed to dry as dust formation can then occur. * Wear safety goggles and tie back long hair.

  2. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    Temperature of H2O2(0C) 1 2 3 4 5 6 Median 5 24 26 25 21 24 28 24.5 20 5 5 7 7 8 8 7 40 5 4 6 5 5 4 5 55 4 5 3 3 3 4 3.5 60 4 3 3 3 2 3 3

  1. Investigate the effect of temperature on the activity of an immobilised enzyme.

    This is so the temperature won't drop as the beads are been left to equilibrate at the temperature. * The milk must be passed over the lactase beads for the same amount of time at the same drop rate for every experiment.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    pipette and a syringe: o For the first concentration I used 1 mole of copper sulphate in its pure state, o For the second one I used 0.75 moles of copper sulphate and diluted it with distilled water until the volume of the solution was the same as for the first concentration.

  1. Investigation into the Effect of Temperature on the Rate of Fermentation by Yeast.

    this was subtracted also; carbon dioxide was produced in this experiment, as can be seen in the table. The total amount of carbon dioxide that was produced in the 60 oC experiment was 53 cm3. The release of carbon dioxide stopped after 300 seconds.

  2. Investigating the Effect of Temperature on Rate of Respiration in Yeast

    It is reliable, accurate and simple. The only problem I found was that the indicator takes a very long time to change colour. The indicator is sensitive to pH changes and indicates when decarboxylase enzymes release carbon dioxide. If the carbon dioxide production is not sufficient, the indicator may even turn purple, which is a totally unwanted result.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work