• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the effect of sucrose concentration on the conversion into glucose and fructose by invertase

Extracts from this document...


Investigating the effect of sucrose concentration on the Conversion into Glucose and Fructose by Invertase Prediction I would expect to find that an increase of sucrose concentration would lower the time taken for the clinistix to turn blue from red, or said in other words, the rate of reaction would increase with an increase of substrate concentration until a certain point where maximum rate of reaction will be reached. Higher concentration of sucrose has more molecules inside it therefore it is more likely that a collision will take place, molecules must collide in order to react. This means that a reaction is more likely to take place in a shorter time, making the rate of reaction quicker until it reaches the maximum rate of reaction, where increase in substrate concentration would not increase the reaction rate because at this point all the active sites are full. (1) Analysing Evidence By looking at my graph showing the Average Time Taken (s) for the clinistix to turn blue against Sucrose concentration (%), I can say that the general trend seems to be that as the sucrose concentration increases, the average time taken for the clinistix to turn blue, decreases. This is shown on my graph by a linear relationship that goes down (negative correlation). But for more detailed interpretation I will concentrate on my second graph, which shows the Rate of Reaction against Sucrose Concentration: Between 0.5% and 1%, there is a big increase in the reaction rate, as concentration is doubled. ...read more.


* The equipment needed to measure the time and the sucrose solution is not mentioned in the method. * The method does not exactly say, when to place the clinistix in the sucrose solution. * The method used doesn't tell us how many of the sucrose filled test tubes to test at a time, and also how to time the time taken for the clinistix to turn blue. * The method does not suggest how to make sure that the colour level of each clinistix is the same. * Also the method does not mention washing up of the equipment whilst using it and does not account for safety precautions What improvements would I make to the method or equipment Used? If I were asked to repeat the experiment I would change the method and equipment in the following ways: 1. I would include a detailed list of the equipment needed. For example I would say that 2 cm3 pipette is needed to measure the sucrose solution, and also I would say that I would use a stop clock to measure the time taken for a colour change in the clinistix to occur. 2. I would say that I would use a distilled water to prepare the different sucrose concentrations, by mixing it to equal amount of sucrose. ...read more.


To substitute the anomalous result I decided to take the average of the two additional tests and disregard the anomalous. I will use my new result further on in the analysing evidence. The results of the two additional tests were identical (162 sec), giving the same number as an average (162sec), which I believe to be a consistent result. Now by looking at my results I see that at 4% from the same trial the time taken (160sec) is less than the time taken at 8% (162sec). This is not following the main pattern, but it doesn't affect the average results, which are still showing the general trend. The reason for the anomalous result at concentration of 8% might be because of many things. But the main reason for this result is that I wasn't sure if the clinistix had reached the same level of colour, like the other clinistix, so I decided to leave it in for longer, and this is where I made a mistake because the colour didn't change more than it was before. The reason for my uncertainty might have been because I've used different clinistix from the ones I used before. The result at 4 % I don't consider as a big anomaly, as it did not affect the average results. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. To find out how different concentrations of sucrose solution affect the incipient plasmolysis of ...

    Therefore this is the isotonic point for the swede, theoretically the isotonic point which has been found for the length of the Swede cylinder should be the same as the isotonic point for the mass however taking into account other factors which will be discussed later on in the evaluation

  2. Investigating the Effect of Glucose Concentration On the Rate of Reproduction of Yeast Cells

    Lag phase b) Logarithmic or exponential phase c) Stationary phase- of variable length d) Decline phase The units/numbers are variable. This would produce a population curve throughout the duration of existence of a species (for a simple case like the one if we are looking at)


    This is a waste product of respiration. These are the main enzymes involved in respiration. Now that I have looked at the different types of enzymes, it is essential that I am aware of the factors which affect the activity of enzymes.

  2. Investigate the effect of sucrase concentration on the rate of hydrolysis of sucrose.

    The enzyme can be used again. Enzymes are specific to a particular reaction. Most enzymes are globular proteins with one or more active sites. The active site is a region within the enzyme molecule to which the substrate binds. The stress being applied to the substrate, induced to fit into the enzyme, causes the bonds to break in the substrate.

  1. Applied Science

    and potassium (intracellular) ions behave as impermeant solutes, and cerate an osmotic pressure. For example since most cell membrane in the body is freely permeable to water, a decrease in extracellular sodium ion concentration will cause a net movement of water from the extracellular compartment into the intracellular compartment by osmosis.

  2. Affect of sucrose concentration on the rate of respiration.

    The maximum velocity of a reaction is reached when the active sites are almost continuously filled. Increased substrate concentration after this point will not increase the rate. Reaction rate therefore increases as substrate concentration is increased but this is only true up to a certain point and after this the level of products formed e.g.

  1. Investigate the factors affecting the rate of breakdown of sucrose by the enzyme sucrase ...

    I believe that the concentration of substrate is inversely proportional to the rate, but this is just a prediction. This could be a factor to investigate. Volume of enzyme (Keeping the volume of sucrose and concentration of both the same)

  2. Investigation of the effect of adding different concentrations of NaCl to an enzyme-substrate (amylase-starch) ...

    Without the presence of enzymes many reactions in the cell would be too slow to sustain life and many reactions would require very extreme conditions in order to occur; most cells would be destroyed. Most enzymes are globular proteins made up of folded peptide chains.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work