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# Investigating the effect of sucrose concentration on the conversion into glucose and fructose by invertase

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Introduction

Investigating the effect of sucrose concentration on the Conversion into Glucose and Fructose by Invertase Prediction I would expect to find that an increase of sucrose concentration would lower the time taken for the clinistix to turn blue from red, or said in other words, the rate of reaction would increase with an increase of substrate concentration until a certain point where maximum rate of reaction will be reached. Higher concentration of sucrose has more molecules inside it therefore it is more likely that a collision will take place, molecules must collide in order to react. This means that a reaction is more likely to take place in a shorter time, making the rate of reaction quicker until it reaches the maximum rate of reaction, where increase in substrate concentration would not increase the reaction rate because at this point all the active sites are full. (1) Analysing Evidence By looking at my graph showing the Average Time Taken (s) for the clinistix to turn blue against Sucrose concentration (%), I can say that the general trend seems to be that as the sucrose concentration increases, the average time taken for the clinistix to turn blue, decreases. This is shown on my graph by a linear relationship that goes down (negative correlation). But for more detailed interpretation I will concentrate on my second graph, which shows the Rate of Reaction against Sucrose Concentration: Between 0.5% and 1%, there is a big increase in the reaction rate, as concentration is doubled. ...read more.

Middle

* The equipment needed to measure the time and the sucrose solution is not mentioned in the method. * The method does not exactly say, when to place the clinistix in the sucrose solution. * The method used doesn't tell us how many of the sucrose filled test tubes to test at a time, and also how to time the time taken for the clinistix to turn blue. * The method does not suggest how to make sure that the colour level of each clinistix is the same. * Also the method does not mention washing up of the equipment whilst using it and does not account for safety precautions What improvements would I make to the method or equipment Used? If I were asked to repeat the experiment I would change the method and equipment in the following ways: 1. I would include a detailed list of the equipment needed. For example I would say that 2 cm3 pipette is needed to measure the sucrose solution, and also I would say that I would use a stop clock to measure the time taken for a colour change in the clinistix to occur. 2. I would say that I would use a distilled water to prepare the different sucrose concentrations, by mixing it to equal amount of sucrose. ...read more.

Conclusion

To substitute the anomalous result I decided to take the average of the two additional tests and disregard the anomalous. I will use my new result further on in the analysing evidence. The results of the two additional tests were identical (162 sec), giving the same number as an average (162sec), which I believe to be a consistent result. Now by looking at my results I see that at 4% from the same trial the time taken (160sec) is less than the time taken at 8% (162sec). This is not following the main pattern, but it doesn't affect the average results, which are still showing the general trend. The reason for the anomalous result at concentration of 8% might be because of many things. But the main reason for this result is that I wasn't sure if the clinistix had reached the same level of colour, like the other clinistix, so I decided to leave it in for longer, and this is where I made a mistake because the colour didn't change more than it was before. The reason for my uncertainty might have been because I've used different clinistix from the ones I used before. The result at 4 % I don't consider as a big anomaly, as it did not affect the average results. ...read more.

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