• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month


Extracts from this document...


INVESTIGATING THE EFFECT OF TEMPERATURE ON CATALASE ACTION AIM I will be investigating how temperature affects the rate at which catalase catalyses hydrogen peroxide. The reaction is: Catalase + Hydrogen peroxide Water + Oxygen PREDICTION I predict that as the temperature increases, the rate of reaction between the (enzyme) catalase and the (substrate) hydrogen peroxide will increase. This is because they will have more kinetic energy, which will make them move faster and collide together with much force. But if temperature rises above 40�C, the rate of reaction will decrease due to the denaturing (alteration) of the protein structure resulting from the breakdown of the weak ionic and hydrogen bond that stabilize the 3 dimensional structure of the protein. The graph shows the rate of an enzyme catalysed reaction. At low temperature at point C, the reaction is taking place very slowly due to molecules moving relatively slowly. At this point, molecules won't often collide with the active site, so binding between substrate and enzyme is very rare. As temperature rises at point B, the enzyme and substrate molecules move faster. Collisions happen more frequently, so that substrate molecules enter the active site more often. Moreover, when they collide, they do so with more energy. This makes it easier for bonds to be broken so that the reaction can occur. ...read more.


For fair test because it has clear marking point for measuring. Bowl 1 To use as the site of displacement. Thermometer 1 To measure the water bath temperature. To get the right temperature for the investigation. Pipette 1 � 25ml To measure the volume of hydrogen peroxide and yeast. For accurate measurement. Clamp 1 To hold the burette in a horizontal position. PH buffer 15cm� To keep the ph level of the yeast constant. Make the yeast to be not too acidic or too alkaline during the experiment. So as to get the result accurate. Beehive shelf 1 To hold the burette and delivery tube in place VARIABLES VARIABLE WHY DOES IT NEED TO BE CONTROLLED HOW WILL IT BE CONTROLLED Temperature So that effect of different temperatures can be investigated. By using a thermostatic controlled water bath. Volume of yeast So as to keep the same volume of enzyme in the experiment, so as to get an accurate result. This is because if there's more catalase, the reaction will occur faster as more enzyme substrate complex will be formed. Carefully measure out 50cm� of yeast with a pipette. Volume of hydrogen peroxide If more hydrogen peroxide is used, the enzyme will have an increased chance of binding with the substrate therefore increasing the chance of a successful collision. ...read more.


CONTROL Whilst carrying out this experiment, do the followings in order to make the experiment fair: 1. Use the same concentration of yeast and same amount of yeast. 2. Use boiled yeast for standard control by getting the yeast to a point where it gets denatured. Then add the denatured yeast to the hydrogen peroxide. If any gas gets produced, then subtract the volume of gas produced (during the reaction with the denatured yeast) from the result derived when carrying out the real experiment. This is also done so as to be sure that there's nothing else in the yeast producing gas except the enzyme so that the experiment isn't affected. 3. Use the same volume of hydrogen peroxide and concentration. 4. Use the same equipment set up for fair result. 5. Have the same time span for the reaction to take place (3minutes). RESULT TABLE FIRST TEST Temperature of the yeast (�C) Volume of gas produced for up to 3 minutes (cm�) 40 50 60 70 80 SECOND TEST Temperature of the yeast (�C) Volume of gas produced for up to 3 minutes (cm�) 40 50 60 70 80 THIRD TEST Temperature of the yeast (�C) Volume of gas produced for up to 3 minutes (cm�) 40 50 60 70 80 AVERAGE RESULT Temperature of the yeast (�C) Volume of gas produced (cm�) First test Second test Third test Average result 40 50 60 70 80 ?? ?? ?? ?? BIOLOGY PLAN ABI LUGBOSO - 1 - ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Energy, Respiration & the Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Energy, Respiration & the Environment essays

  1. Marked by a teacher

    effect of temperature on the rate of respiration in yeast

    5 star(s)

    For this to happen, respiration goes under few processes such as: Glycolysis: Glycolysis is the sequence of reactions that converts glucose into pyruvate with the concomitant production of a relatively small amount of ATP. Firstly glucose is phosphorylated from 2 ATP molecules to raise the energy level of the compound

  2. Marked by a teacher

    An investigation into the effect of different sugars on respiration in yeast.

    5 star(s)

    * Using a graduated pipette, take 9cm3 of yeast and place in a boiling tube. Keep in the water bath. * Using a clean pipette, take 1cm3 of the sugar solution and place in the boiling tube with the yeast.

  1. Marked by a teacher

    Effect of nitrate concentration on the growth of Duckweeds

    5 star(s)

    provides the H ions, without these ions the Calvin Cycle cannot take place. This Light- independent reaction is where the main manufacture of sugars and carbohydrates takes place, with the help of the reduced NADP and energy providing ATP molecule.

  2. Marked by a teacher

    Fighter Pilot A Statistical Analysis of Reaction time and its Correlation with Dominant ...

    5 star(s)

    to be made and gave an idea of possible changes within the variables before performing the actual test. A group of ten women of a young age were used, although finding subjects that would pass the above criteria proved to be somewhat more difficult than expected and a leniency on the controlled variables may have to be allowed.

  1. What effect does substrate have on respiration in yeast?

    flask 100ml Conical flask To hold the 10ml of yeast solution and 1g of the substrate during the experiment Rubber tubing of appropriate length One end to be attached to the conical flask and the other end to be inserted into the inverted graduated cylinder Rubber stopper with single hole To be placed on the mouth of the conical flask.

  2. the effect of bile concentration on the activity of the enzyme lipase during the ...

    pH - pH is a measure of the acidity or hydrogen ion concentration of a solution. It is measured on a scale of 0-14 with pH values below 7 being acidic, values above 7 being basic and a value around 7 is neutral.

  1. An investigation into the effects of temperature on the rate of anaerobic respiration of ...

    10 3.7 10.9 13.5 15 4.7 14.1 18.5 20 5.6 17.7 21.9 25 6.2 20.9 24.7 30 7.3 23.1 27.6 35 8.1 26.2 29.5 40 9.4 27.9 31.6 45 9.8 29.7 33.2 50 10.7 30.9 34.2 My average results suggest that 25 minutes in the water bath at 20 �C,

  2. Investigate the effect of bile salt concentration on the digestion of milk by the ...

    As the temperature increases, the vibrational energy also increases. This affects the secondary and tertiary structures bonds, weakening them and eventually breaking them. The weakest bonds break first such as hydrogen bonds, and the stronger bonds break later. This is why enzyme activity drops quickly but not immediately as the enzyme is denatured gradually.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work