Investigating the effect of temperature on catalyse

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Biology coursework: Investigating the effect of temperature on catalyse

Aim: the aim of this experiment is to find the effect of temperature on the rates at which catalyse work and the optimum temperature they work at.

This experiment tests the rate at which hydrogen peroxide breaks down to form oxygen and water using either Liver catalyse.

2H2O2(aq)                        2H2O(l) + O2(g)

Preliminary work

The importance of the preliminary work was to test the variables chosen for the experiment were sensible and reliable.

  • Preliminary 1: variable of time duration
  • This tested how long we measured the release of oxygen for, either until no more oxygen is released or to specific time duration.  The tables below show the results we found.

Predictions

I predict that more oxygen will be produced in the 30 seconds when the catalyse and the substrate are heated at around 40ºC, this prediction is based on previous research

Equipment

1)         Blender:

I will use a  blender to blend the liver to reduce the divergences such  as surface area

2)         Liver:

Liver is the catalyst I use to catabolically breakdown Hydrogen Peroxide

3)        Test tubes:

4)         Conical flask 500cm3

                I will pput the

5)         Water bath:

I will use a water bath to adjust the liver catalyst and the Hydrogen peroxide to the required temperature.

6)        Thermometer:

I will use a thermometer to check if the liver and the Hydrogen peroxide test tubes have been given enough time in the water bath or the ice beaker to adjust to the required temperature.

7)        Stopwatch

I will use a stop watch to check when the time duration for the reaction.

8)        Conical flask and bung

9)        Pipette

will use a pipette to move the hydrogen peroxide to the measuring cylinder

10)        Measuring cylinder

I will use a measuring cylinder to measure the right amount of hydrogen peroxide (2ml3) and liquidised liver (10 ml3)

11)        Delivery tube

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12)        Gas syringe (100cm3)

I will use a gas syringe to measure the amount of oxygen released in 30 seconds when I react the hydrogen peroxide with the liquidised liver

13)        Hydrogen peroxide

I will be using hydrogen peroxide as the substrate which the liver enzymes will catabolically breakdown.

14)        Distilled water

I will add distilled water to the blender when liquidising the liver for dilution purposes.

15)        Chemical waste bowl

I will use a chemical waste bowl to dispose of my chemicals such as H2O2 and liver as these chemicals could cause problems if disposed of carelessly.

16)        Plastic disposable ...

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