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Investigating the effect of temperature on catalyse

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Biology coursework: Investigating the effect of temperature on catalyse Aim: the aim of this experiment is to find the effect of temperature on the rates at which catalyse work and the optimum temperature they work at. This experiment tests the rate at which hydrogen peroxide breaks down to form oxygen and water using either Liver catalyse. 2H2O2(aq) -->2H2O(l) + O2(g) Preliminary work The importance of the preliminary work was to test the variables chosen for the experiment were sensible and reliable. * Preliminary 1: variable of time duration o This tested how long we measured the release of oxygen for, either until no more oxygen is released or to specific time duration. The tables below show the results we found. Predictions I predict that more oxygen will be produced in the 30 seconds when the catalyse and the substrate are heated at around 40�C, this prediction is based on previous research Equipment 1) Blender: I will use a blender to blend the liver to reduce the divergences such as surface area 2) Liver: Liver is the catalyst I use to catabolically breakdown Hydrogen Peroxide 3) Test tubes: 4) Conical flask 500cm3 I will pput the 5) Water bath: I will use a water bath to adjust the liver catalyst and the Hydrogen peroxide to the required temperature. ...read more.


After research and careful consideration, I have decided to first look at how a change in temperature could affect the rate of reaction. In order to design a suitable experiment and make a credible prediction, I must first explore more closely how temperature is likely to affect the rate of catalysis. Enzymes are specific - they only control one type of reaction; therefore I must use one specific enzyme in my experiment, in order to find a clear way of measuring the rate of reaction. Although they are specific, all enzymes work in a very similar way and have similar properties. They are all globular proteins and are all biological catalysts, they increase the rate of a given reaction without being used up and their presence does not change the nature of the reaction or the end product. Enzymes work by having an active site, made from amino acids. Here, substrate molecules will bind with the enzyme (and other substrate molecules if necessary) and a reaction takes place. The enzyme itself is not affected and releases the new chemical after the reaction. After release of the end product, more substrate molecules can bind with the active site. Enzymes can catalyse anabolic reactions or catabolic reactions (involved in breakdown). The diagram above shows an anabolic reaction. ...read more.


3) a. Once one temperature set of liver/Hydrogen peroxide have been allowed to adjust to the temperature required pour the liver into a conical flask and put the bun on then pour the H2O2 in through the funnel and cover the top with your hand. Then start the stopwatch timing 30 seconds. (see problem A) b. Once the 30 seconds has passed, move my hand from the top of the funnel and note down the volume of oxygen collected in the gas syringe. (See Problem B and C). (repeat stage 3 for each set of temperatures) Results Temperature (oC) 1st 2nd 3rd 4th Average standard deviation 10 11 9 12 9 10.25 1.5 Room (20) 20 27 19 27 23.25 4.3493 30 36 35 35 17 35.33 9.1788 40 49 42 42 44 44.25 3.304 50 16 19 17 15 16.75 1.7078 Analysis Upon analysis of the table of results I can determine that my predictions were reasonably accurate and that, as predicted the most gas was released in the 30 seconds time period when the liver and the hydrogen peroxide were adjusted to the temperature of 40�C this is because it is closes to the body temperature of 37�C maintained by homeostasis. However there were minor flaws in my prediction as there was small amount of oxygen released when the temperature was at 50�C, this could nevertheless have been caused by the problem of displacement of oxygen when the hydrogen peroxide was added to the liver. ...read more.

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