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Investigating The Effect of Temperature on Enzyme Activity.

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Introduction

Investigating The Effect of Temperature on Enzyme Activity Aim: To see whether there is a clear effect, if any upon the different temperatures an enzyme, trypsin works in. Introduction: In this coursework experiment, we are testing to see whether different temperatures affect the way an enzyme works. By this, we mean whether the enzyme is still able to break down the large insoluble molecule, whether the rate of reaction changes, or if the lock and key still works. The main things that we will be looking for are accuracy, reliability, and fair testing. All these things combined together will show us the real effect of temperature on enzymes. By using my previous knowledge about enzymes, I think that once we allow the enzyme to work in temperatures above 40�c, the enzymes will not break down as much molecules as before. This will be partly due to the fact that the rate of reaction will slow dramatically. The main reason for this sort of activity is because the enzyme will become denatured, and thus the lock and key procedure will not be carried out. To create a fair test, we will be testing each temperature three times. In addition, we will be testing it against a control. ...read more.

Middle

The acclimatisation period was just right, as it heated the test tubes to the right temperature, and would allow us to have four experiments for each temperature in the time provided. Also it is a round number, and easy to plot on a graph. 2. We found out that the volume of the solution being used was also correct, as in theory; the solution was made of equal parts of buffer and trypsin. Too much, or too little solution would drastically change the results we would expect. 3. The temperatures used are good because they are round, and in ratio to each other. This means that they are easier to plot on a graph. Main Experiment Aim: To complete three tests and one control test of each temperature for a solution of trypsin and buffer. Apparatus: * 20 clean and dry test tubes * 30 cm� of water * 80 cm� of buffer and trypsin solution * 20 split ended splints * 20 pieces of equal sized film * 1 stop watch * 3 water baths set at pre-heated temperatures * Results table Diagram: Method: 1. Fill 15 test tubes of buffer and trypsin solution. The volume must be exactly 5 cm� in each. 2. Fill 5 test tubes of water to act as a control for each experiment. ...read more.

Conclusion

A sterile syringe was used to improve accuracy. Also, the trypsin and buffer solution were of equal parts, and were not mixed with any other substances. I feel that I recorded my results in an appropriate manner, using the right titles, space, and alignment. In the overall experiment, the right values were used, and also the right quantities of each substance. To further emphasis the need for accuracy, I repeated each test three times to create an average, which would be more accurate than just a single test. This is because if an anomaly is found, then it would be easier to spot, and therefore easier to correct. The main strength in this coursework of me was accuracy. I feel that I was always accurate, fair, and decisive. All the volumes, times, and calculations were done in a thorough and precise manner. The only weakness that I had in this coursework was the fact that I wasted too much time in preparation, and also in between test. In the overall outcome, this accounted to about ten or fifteen minute, which are valuable in this type of circumstances. Improvements for other work include: * Smaller time gaps in which to record a change in the state of the film. * Less time to be wasted during and around tests. For example, acclimatisation periods. ...read more.

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