Investigating the Effect of Temperature on Rate of Respiration.

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* Biology A2 Coursework Name: Luke Meredith

Form:13 CJN

PLANNING

Title: Investigating the Effect of Temperature on Rate of Respiration

Aim: I am to investigate and take readings of oxygen consumption, on an organism at several temperatures including replicate readings to give a mean value. At each temperature I will leave the invertebrates for about 10 minutes for the rate of respiration to reach equilibrium.

Diagram

Experiment 1

Experiment 2

Partial 2b .

Complete 4c.

Apparatus List:

Test tubes x2 Invertebrates (maggots)

Respirometer Data Harvesting software on computer and probe

Gauze platform clamp stands

1cm syringe

Glass beads

Sodium hydroxide

.

Preliminary Work Conducted

The first diagram shows two test tubes linked to respirometer. One test tube contains invertebrates (in this situation, maggots) with sodium hydroxide underneath separated by a gauze platform. The other contains glass beads and also sodium hydroxide as shown on the diagram. The glass beads act as inert material to the volume of the invertebrates. Using this method I could measure the rate of respiration at different temperatures, of these invertebrates, by changing the