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Investigating the effect of temperature On the action of Amylase on Starch.

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Name: Samerah Saeed Candidate No. 4214 Form: 10N Centre No. 12101 Investigating the effect of temperature On the action of Amylase on Starch. Aim We are trying to find out the effect of the temperature on the reaction rate of Amylase and Starch. An enzyme is a biological catalyst (a chemical that accelerates or speeds up a chemical reaction or living process). The enzyme we will be using is called Amylase. Enzymes breakdown and build up molecules. They are reusable and made up of proteins. The substrate we will be using is starch; enzymes break substrates in to smaller molecules. All enzymes have an active site. Each active site is unique to the type of substrate it will fit it exactly. Scientists use the key and lock idea to demonstrate the exact binding of a substrate to the active site of an enzyme. The substrate must fit the enzyme exact for an action to take place. There are two factors that affect the rate of reaction. These are pH and the temperature. Enzymes usually work best at a pH of 7 (neutral), and a temperature of around 20 C -30 C. Starch is a type of Carbohydrate it is a polysaccharide, which means it is very complex. It is made up of a chain of sugar (glucose) molecules. It can only be broken down by Carbohydrase. It is detected using iodine, if it is found present then the iodine turns a blue/black colour if not then it turns orange/red. ...read more.


* Amount of Starch * Amount of Amylase * Condition of water bath * Concentration of Starch (1%) * Concentration of Amylase (1%) Fair Test I am going to keep this experiment fair by keeping the following variables controlled, such as the amount of Starch and Amylase used in each of the seven temperatures. Also the conditions of the water baths will be kept the same. I will also use the same concentration of Starch and Amylase (1%). The pH of the experiment will also be kept the same. For accuracy I am going to conduct each experiment three times, repeating the experiment will allow errors, and also help to gain more accurate results. I will then plot the graph according to the average readings we get from each set of results. Measurements We are going to measure the rate of reaction at the following temperatures; 0 C, 10 C, 20 C, 30 C, 40 C, 50 C and 60 C. The measurements will be taken at 10 C intervals. The temperatures will be set using a thermometer. Also water baths will also be set up to specific temperatures. Time A stopwatch will be used to monitor the rate of reaction. The reaction rate will be measured in minutes and seconds. We will take a sample of Starch mixed with Amylase every 20 seconds, and test it with iodine. Volume The Starch and Amylase will be measured using a very accurate measuring cylinder that uses ml units as a scale. ...read more.


This would have been due to the fact that there was not enough energy at 0 C, and that the enzyme was denatured at 60 C. The graph of results would have been different; there would have been a very slow rise in peak and a slower trough. I do not think that my method was very good there were many opportunities for the Starch and amylase to mix before I had begun the experiment. Also measuring out the solutions could have been done more accurately to the nearest 0.1ml. Also the water baths for the experiments at 0 C, 10 C and 20 C did not stay the same temperature. We had to make sure every so often that the temperature was constant; we did this by using ice. If I did this experiment again I would include theses two steps. 1. Ensure that by keeping the measuring cylinders separate they do not get contaminated. Causing the Amylase and Starch to mix before the experiment begins. 2. Ensure that the water baths all remain at a constant temperature regularly. Also we did not know for sure when all of the Starch had been broken down this was because we took a sample every 20 seconds. To gain more accurate results we could have taken a sample every 5 seconds instead, to find out for sure exactly when the Starch had all been broken down. Other experiments on the same idea are to see how pH or the concentration of a solution affects the reaction rate between a substrate and enzyme. ...read more.

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