• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of Temperature on the Enzyme Catalase.

Extracts from this document...


Investigating the Effect of Temperature on the Enzyme Catalase Inside our bodies thousands of chemical reactions take place in our cells. We need these reactions to happen quickly to keep us alive. Normally these reactions would be to slow but thanks to enzymes they can be performed much more quickly. And the enzyme doesn't get used up so it can move on to perform many more times. There are two main groups of enzyme, the ones that break molecules up, and ones that put molecules together. The enzyme we are looking at, Catalase is in the first category and is so called a catalyst. Since enzymes speed up chemical reactions in our bodies there often called biological catalysts. Enzymes work on substances called substrates. The reaction takes place on a part of the enzyme called the active site, which is often described as a lock and key mechanism, because the enzyme will only work with the substrate that it was made for. So Catalase will only break down hydrogen peroxide and nothing else. If our bodies didn't contain any Catalase we would most certainly die, because one of the by-products of respiration is hydrogen peroxide (a bleach) which can be very harmful if not dealt with. ...read more.


Then because of the reaction going on which I will explain later, oxygen bubbles form and lift the piece of filter paper back to the surface. When the filter paper reaches the surface we then stopped the clock. Now with our first few experiments that we did, we just heated the potato extract but left the Hydrogen peroxide at room temperature. This proved to give very similar results even with different temperatures. So we then decided to heat both the potato extract and the Hydrogen Peroxide which proved to be much more successful. Explanation Behind the Experiment The reason behind the filter paper floating back to the surface is, that when the catalase is added to the H202 it breaks down a bond in the compound leaving you with H20 and 0 (water and oxygen). The oxygen is formed above and below the filter paper but gets trapped underneath. When enough oxygen has gathered underneath the filter paper it then rises back to the surface. Investigating the Effect of Temperature on the Enzyme Catalase Preliminary experiment Temp of enzyme Test one Test two Test three Average 20�C 26 seconds 27 seconds 28 seconds 27 seconds 30�C 28.5 seconds 29.1 seconds 32 seconds 29.8 seconds 40�C 48 seconds 76 seconds - - 50�C - - - - 60�C - - - - After having done eight ...read more.


And because the temperature isn't too high the enzymes won't de-nature. My prediction was that the enzyme would work best around body temperature then get worse, which wasn't to far of my results. Evaluation The way in which we conducted the experiment was reasonably controlled but the amount of catalase on the filter paper could have varied slightly. Investigating the Effect of Temperature on the Enzyme Catalase The procedure could have also been improved by using fresh H2O2 with each experiment, because some of the hydrogen peroxide will have been broken down into oxygen and water. This would have then diluted the hydrogen peroxide. Overall the procedure gave reasonably reliable result with some anomalies that didn't affect the average too much. Further experiments could involve trying lower and higher temperatures, to see which temperature the enzyme would work most efficiently at and what temperature the enzyme becomes fully denatured at. Also if I were to repeat the experiment I would do more repeats of each experiment to make the average more accurate. Testing with smaller gaps in temperatures i.e. 5oC gaps rather then 10oC gaps. Would show more precisely when the enzyme becomes denatured and when it's at its most efficient. Also the use of a water-bath would have given more accurate results, because it can keep things at a constant set temperature. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The effect of temperature on the enzyme Catalase.

    4 star(s)

    All enzymes are made inside cells, and most of them are left in the cells to speed up reactions in the cytoplasm and nucleus. These cells are called intra-cellular enzymes. There are also extra cellular enzymes, which are let out of the cells and work outside of the cell.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    as this is clearer than drawing parallelograms all over the graph paper. (v) Gradient of line between 1 and 2 minutes for 25% Celery Extract concentration = 0.8 cm3 22.5 seconds = 0.035555555 = 0.0360 cm3/sec (3 sig. fig) (vi)

  1. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    Increasing the number of collisions or increasing the speed of the collisions will increase the rate of reaction. Little diagram of the temperature particles. As the temperature increases, there is more collision and the particles react faster with enough energy.

  2. To examine the effect of temperature on the enzyme catalase.

    urea alcohol and others. However, that does not mean that the lower the temperature the better it will perform. Like all reactions the rate of decomposition of H2O2 will increase with heat. This is further increased when in the presence of the enzyme. So we can see that whilst a higher temperature is favourable,

  1. The Effect of Temperature on Catalase Enzyme.

    Different pieces liver will not have exactly the same water and catalase content. The mass will be kept constant in the same way as surface area and concentration of enzyme. Prediction: The higher the temperature, the higher the rate of reaction up to a certain point.

  2. Find the effect of temperature on enzymes, using a potato as a catalyst. The ...

    The catalytic activity of an enzyme is determined primarily by the enzyme's amino-acid sequence and by the tertiary structure-that is, the three-dimensional folded structure of the macromolecule. Many enzymes require the presence of another ion or a molecule called a cofactor, in order to function.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work