• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of Temperature on the Enzyme Catalase.

Extracts from this document...

Introduction

Investigating the Effect of Temperature on the Enzyme Catalase Inside our bodies thousands of chemical reactions take place in our cells. We need these reactions to happen quickly to keep us alive. Normally these reactions would be to slow but thanks to enzymes they can be performed much more quickly. And the enzyme doesn't get used up so it can move on to perform many more times. There are two main groups of enzyme, the ones that break molecules up, and ones that put molecules together. The enzyme we are looking at, Catalase is in the first category and is so called a catalyst. Since enzymes speed up chemical reactions in our bodies there often called biological catalysts. Enzymes work on substances called substrates. The reaction takes place on a part of the enzyme called the active site, which is often described as a lock and key mechanism, because the enzyme will only work with the substrate that it was made for. So Catalase will only break down hydrogen peroxide and nothing else. If our bodies didn't contain any Catalase we would most certainly die, because one of the by-products of respiration is hydrogen peroxide (a bleach) which can be very harmful if not dealt with. ...read more.

Middle

Then because of the reaction going on which I will explain later, oxygen bubbles form and lift the piece of filter paper back to the surface. When the filter paper reaches the surface we then stopped the clock. Now with our first few experiments that we did, we just heated the potato extract but left the Hydrogen peroxide at room temperature. This proved to give very similar results even with different temperatures. So we then decided to heat both the potato extract and the Hydrogen Peroxide which proved to be much more successful. Explanation Behind the Experiment The reason behind the filter paper floating back to the surface is, that when the catalase is added to the H202 it breaks down a bond in the compound leaving you with H20 and 0 (water and oxygen). The oxygen is formed above and below the filter paper but gets trapped underneath. When enough oxygen has gathered underneath the filter paper it then rises back to the surface. Investigating the Effect of Temperature on the Enzyme Catalase Preliminary experiment Temp of enzyme Test one Test two Test three Average 20�C 26 seconds 27 seconds 28 seconds 27 seconds 30�C 28.5 seconds 29.1 seconds 32 seconds 29.8 seconds 40�C 48 seconds 76 seconds - - 50�C - - - - 60�C - - - - After having done eight ...read more.

Conclusion

And because the temperature isn't too high the enzymes won't de-nature. My prediction was that the enzyme would work best around body temperature then get worse, which wasn't to far of my results. Evaluation The way in which we conducted the experiment was reasonably controlled but the amount of catalase on the filter paper could have varied slightly. Investigating the Effect of Temperature on the Enzyme Catalase The procedure could have also been improved by using fresh H2O2 with each experiment, because some of the hydrogen peroxide will have been broken down into oxygen and water. This would have then diluted the hydrogen peroxide. Overall the procedure gave reasonably reliable result with some anomalies that didn't affect the average too much. Further experiments could involve trying lower and higher temperatures, to see which temperature the enzyme would work most efficiently at and what temperature the enzyme becomes fully denatured at. Also if I were to repeat the experiment I would do more repeats of each experiment to make the average more accurate. Testing with smaller gaps in temperatures i.e. 5oC gaps rather then 10oC gaps. Would show more precisely when the enzyme becomes denatured and when it's at its most efficient. Also the use of a water-bath would have given more accurate results, because it can keep things at a constant set temperature. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The effect of temperature on the enzyme Catalase.

    4 star(s)

    Prediction - By using the range of temperatures that I am (15�c-50�c), I think that the Catalase will work best and produce the most froth at potato temperature, which is about 15�C. I think this because in our theory I learned that over 40�C the Catalase enzyme breaks and denatures,

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    I tried to avoid this by having an assistant to help me during the reading of my measurements, and also to help assemble everything, but even so some slight inaccuracies could have been caused by human error. However, if there are any, they are so insignificant that it is relatively unimportant.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    They would have been noted down every thirty seconds for 2 minutes so that I had four results, but they were recorded every minute for three minutes, to get three results. Again my method would have provided a wider range of results, which would have made a smoother curve on a graph, showing where anomalous results are more easily.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    1 M concentration Experiment 1 Experiment 2 Experiment 3 Average Standard Deviation Coefficient of variation Time (s) Volume (ml) Volume (ml) Volume (ml) Volume (ml) Volume (ml) % 30 11.0 10.5 9.5 10.3 0.8 7.4 60 12.5 11.5 11.0 11.7 0.8 6.5 90 13.0 13.0 12.5 12.8 0.3 2.2 120

  1. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    For example the enzyme catalase reacts with hydrogen peroxide forming the products water and oxygen and this process is repeated over and over again with the same enzyme molecule. * An enzyme does one of the two things, it either splits a complex substrate molecule into two simple ones or

  2. The Effect of Temperature on Catalase Enzyme.

    Different pieces liver will not have exactly the same water and catalase content. The mass will be kept constant in the same way as surface area and concentration of enzyme. Prediction: The higher the temperature, the higher the rate of reaction up to a certain point.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work