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Investigating the effect of temperature on the movement of pigment through cell membranes.

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Introduction

Investigating the effect of temperature on the movement of pigment through cell membranes Results Different groups of data recording the transmission in % Temperature 1 2 3 4 5 6 7 8 9 Group Average/2d.p 25 56 56 56 72 34 63 / / 62 57 35 58 52 50 58 36 55 / 55 50 51.75 45 50 39 46 47 29 50 30 70 65 47.33 55 / 12 20 16 15 42 21 15 44 23.13 65 32 6 0 5 4 17 6 4 41 12.78 75 4 2 0 4 3 6 0 0 14 3.67 85 4 0 0 4 2 2 0 0 3 1.67 Group 2 are the results that we obtained. Analysing evidence and drawing conclusions Transmission in this case means, when we used the colorimeter it is the percentage of light that was able to go through the solution of water and beetroot pigment. I have spilt the graph of my results up into three sections so that it is easy to explain what is going on inside the cells. Also because there are three distinct sections on the line of best fit of my results. There is one major trend in the results that were obtained and it was that there was a large dip in the transmission results mid way through the results. ...read more.

Middle

Therefore the cell membrane wasn't intact and large holes have occurred. Seeing as there is still a greater concentration of pigment inside the cells the pigment diffuses out of the cell into the water. Therefore the pigment has diffused with the concentration gradient going from high concentration to low concentration. There is now no restriction to the size of the pigment particle, which means that all the pigment particles no matter the size are able to diffuse out of the cell. Another reason for the pigment getting out of the cell was the fact that the temperature had got so high that the globular proteins in the cell membrane denatured. This meant that there were the holes that pigments made in the cell walls and there were holes that the proteins left after being denatured, this meant that pigments could freely leave the cell. The rise in the temperature also caused the hydrogen bonds in the secondary structure, which hold the protein together, to break. This means that the structure goes down to a primary structure from a secondary structure, the reason being is because the hydrogen bonds that were keeping chains of amino acids together to form a ? (alpha) helix have been broken. ...read more.

Conclusion

The tripod being metal was still conducting the heat it had gained into the water bath meaning that the temperature of the water bath would rise after the Bunsen burner had been turned off. With the temperature rising above the wanted temperature this could have been why the 55?C result was an anomaly. The temperature of the water bath for this result could have increased more than in the other results. Meaning that the pigment particles had more kinetic energy than they should have had, therefore more of the pigment particles might have made a hole in the membrane allowing more pigment out than it should have done. The way that the method of the experiment could have been improved to improve the accuracy of the results would have been to use an electrical water bath. The reason why is because these water baths are able to maintain the set temperature unlike the water bath that we used that increased the temperature after the Bunsen burner had been turned off. Another way of improving the method to improve the accuracy of the results would be to use a device that would be able to shake the test tubes with the same amount of force and frequency for a required period of time. This would mean that each of the experiments would be on a level par with each other and would increase accuracy. ...read more.

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