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Investigating the effect of temperature on the rate of digestion of gelatin by pepsin.

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Introduction

Investigating the effect of temperature on the rate of digestion of gelatin by pepsin Aim: To find out what effect different temperatures have on enzymes. To do this, I have planned an experiment with pepsin (which contains enzymes) to see how it breaks down gelatin(such as the ones found on photographic film), at a variety of different temperatures. Plan: I am going to: � Design and set up an experiment that will effectively monitor the results � Measure and evaluate the results, using graphs � To help me predict what will happen, I am going to read books and gather research into the subject of enzymes. Apparatus � 5 photographic strips (Gelatin), each measuring 0.5cm x 35 mm � 5ml protease (Pepsin) � Test tubes � Water bath � Thermometer � Stopwatch/timer Predictions I think that as the temperature increases, so will the enzyme's rate of reaction, but I think when it gets to a certain temperature, the enzymes will begin to denature and will not react as quickly. ...read more.

Middle

I ended my experiment when the temperature reached 55�C although I carried on doing the experiment to see what temperature the photographic strip denatured at. Fair Test: I ensured this was a fair test by doing the following: � Doing all the tests on the same day because sometimes there are conditions out of our control that can change � Checking that the concentration in the test tube was always the same: 2% pepsin, to ensure balanced results � Making sure the temperature on the thermometer was exactly as it should be when recording my stopwatch results � Using the same type of photographic film and the same height and length Safety Safe Test As well as the experiment needing to be a fair test, it must also be a safe test for it to be successful. I must make sure that safety goggles are worn at all times in the experiment as trypsin would be very damaging if it got into the eye as it breaks down protein and most of the eye is made of protein. ...read more.

Conclusion

A curve of best fit would only be appropriate between 30 and 55�C. These results fitted in with my predictions because the enzymes did increase their rate of reaction when the temperature increased and did denature at a high temperature. Evaluation I thought my experiment worked well because it showed similar readings and I didn't have any odd-looking figures within the results. I also thought the results were accurate enough, because it gave such a clear pattern of the enzymes' behaviour. If I wanted get obtain even more accurate results, I could have tested at an even greater number of temperatures. I think my method was the best way of carrying out the investigation because I took more than two results to reach my conclusion. I could improve my method by taking even more results. To provide me with even more evidence for this investigation, I could compare notes with other students who have carried out the same experiment, to see if there are any anomalies. ...read more.

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