• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the effect of the substrate concentration on the decomposition of Hydrogen Peroxide by the enzyme catalase.

Extracts from this document...

Introduction

Investigating the effect of the substrate concentration on the decomposition of Hydrogen Peroxide by the enzyme catalase. Planning Aim In this experiment, I am going to investigate how the concentration of hydrogen peroxide will affect the rate of the reaction. I am going to use different concentrations of hydrogen peroxide as my variable and keep the amount of the liver the same. Background knowledge The formula for catalase breaking down hydrogen peroxide is 2H2O2 ------------> 2H2O + O2 Hydrogen Peroxide is a chemical compound of Hydrogen and Oxygen and the formula for this is H2O2. When hydrogen peroxide is anhydrous it is a colourless liquid which is a strong oxidizing agent which is harmful as it can blister the skin. Catalase is an enzyme (a biological catalyst) for the conversion of hydrogen peroxide into water and Oxygen. Catalase is found in every living cell as it prevents build up of hydrogen peroxide and protects body tissue from damage by H202. Catalase is very important as hydrogen peroxide is consistently being made in metabolic reactions which are chemical reactions taking place inside each cell, catalase is found in large quantities in the liver as a lot of metabolic reactions occur here. ...read more.

Middle

Liver As it contains high amounts of catalase to break down the hydrogen peroxide. Hydrogen Peroxide The substrate in different concentrations as the variable of my experiment. Stop watch To time how long my experiment will go on which will be 60 seconds each time. Diagram Method 1) Set up apparatus( should look similar to above except without liver or hydrogen peroxide yet) 2) Weigh 1g of mashed up liver on a top pan balance and then put the liver into the conical flask. 3) Cautiously measure out 50ml of hydrogen peroxide at 0% concentration in a measuring cylinder. 4) Pour this into the conical flask and securely seal the bung immediately within 2 seconds so that gas cannot escape and start the stop watch straight away. 5) Now gently swirl the conical flask at 60 seconds take the reading of the amount of oxygen that has collected in the gas syringe 6) Clean out the conical flask thoroughly and repeat experiment with 5, 10, 15 and 20% concentrations of Hydrogen peroxide. 7) Repeat each concentration 3 times so that you acquire more accurate results. 8) Calculate the average of each concentration and make graphs. 9) Then from your results work out the rate of each reaction. ...read more.

Conclusion

If the temperature increases too much for example over 50 degrees Celsius the active sites on the enzymes will change and become denatured. This will make the break down of hydrogen peroxide stop and will give me bad results. I also presume that when the concentration gets to high there will be a point where the enzymes will be working as fast as they can all active sites will be occupied at all times and as of this they will not be able to work any faster this means enzymes will reach there Vmax( maximum rate of a enzyme). I think my results should make a sloping graph to a point where it will not rise anymore. This is because of factors like enzymes reaching there Vmax. I predict my results will show a positive correlation as higher the concentration the faster the rate of the reaction as more oxygen will be produced. Results Gas produced after 1 minute (cm�) Concentration (%) Test 1 Test 2 Test 3 0% 0 0 0 5% 4 3 5 10% 21 26 32 15% 68 85 91 20% 71 82 84 Concentration Average Gas produced after 1 minute(cm�) 0% 0.0 5% 4.0 10% 26.3 15% 81.3 20% 79.0 Concentration Average rate of reaction (cm�/s) 0% 0.0 5% 0.06 10% 0.44 15% 1.355 20% 1.316 Rate= Average Volume of Gas Time Taken Biology Coursework Page 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Enzymes - investigate how the substrate concentration (H2O2) affects the activity of catalase on ...

    3 star(s)

    a reliable and accurate set of results that display the effect changing the dependant variable has to this experiment. Below is a table of how these variables will affect the experiment and how it will be controlled. Variable Why is it a variable How it will be controlled Substrate concentration (Independent)

  2. Marked by a teacher

    Beetroot Practical Write up

    3 star(s)

    test tubes, this is because the beetroot at a 9mm only fits in a boiling tube where as the 8mm fits in a test tube. Since the concentrations have been lowered to the range between 0 and 25, with a gap of 5 between each, then only 5 boiling tubes shall be needed for each experiment hence 15 altogether.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    To avoid spillings that would interfere with the good exit of my experiment. Test tube rack To contain the test tubes I used it to maintain the test tubes in place, in an orderly manner. Graduated syringe x2 10 ml One is for injecting the Hydrogen Peroxide into the test

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    I shall measure all the volumes that I am using, using varying glass pipettes of appropriate sizes. Apparatus: * 250.0cm� of celery extract containing catalase * 500.0cm� of hydrogen peroxide * 250.0cm� of distilled water * Stop watch * 250.0cm� conical flask * 3 x 250.0cm� beakers * Bung with

  1. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    Like the saccharomyces cerevisiae solution, I have chosen to use 15cm3 of buffer solution for each experiment. This is so that the two volumes used are the same, reducing the chances of me getting confused and making any errors. If the volume of distilled water differed to that of the

  2. Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of ...

    In the preparation of my alginate beads (immobilised enzyme) I think that it is important to have an even spread of the enzyme throughout the bead, so I will mix it vigorously. Scientific Knowledge Enzymes exist in all living things. They are composed of polymers of amino acids and are produced in living cells.

  1. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    the substrate, is not the right shape it will not fit in the lock, which is the enzyme. Finally the interactions between the substrate and active site of the catalase enzymes cause the hydrogen peroxide to break down into two smaller products.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    4.Describe in detail, how the experiment will be carried. Someone else following your plans should be able to carry it out exactly as you would do. Include an apparatus list. The experiment will be carried out by one or two person (yet unknown). * We will be adding 15 cm3 of Hydrogen Peroxide into the flat bottomed tube (specimen tube)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work