• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the effect of the substrate concentration on the decomposition of Hydrogen Peroxide by the enzyme catalase.

Extracts from this document...

Introduction

Investigating the effect of the substrate concentration on the decomposition of Hydrogen Peroxide by the enzyme catalase. Planning Aim In this experiment, I am going to investigate how the concentration of hydrogen peroxide will affect the rate of the reaction. I am going to use different concentrations of hydrogen peroxide as my variable and keep the amount of the liver the same. Background knowledge The formula for catalase breaking down hydrogen peroxide is 2H2O2 ------------> 2H2O + O2 Hydrogen Peroxide is a chemical compound of Hydrogen and Oxygen and the formula for this is H2O2. When hydrogen peroxide is anhydrous it is a colourless liquid which is a strong oxidizing agent which is harmful as it can blister the skin. Catalase is an enzyme (a biological catalyst) for the conversion of hydrogen peroxide into water and Oxygen. Catalase is found in every living cell as it prevents build up of hydrogen peroxide and protects body tissue from damage by H202. Catalase is very important as hydrogen peroxide is consistently being made in metabolic reactions which are chemical reactions taking place inside each cell, catalase is found in large quantities in the liver as a lot of metabolic reactions occur here. ...read more.

Middle

Liver As it contains high amounts of catalase to break down the hydrogen peroxide. Hydrogen Peroxide The substrate in different concentrations as the variable of my experiment. Stop watch To time how long my experiment will go on which will be 60 seconds each time. Diagram Method 1) Set up apparatus( should look similar to above except without liver or hydrogen peroxide yet) 2) Weigh 1g of mashed up liver on a top pan balance and then put the liver into the conical flask. 3) Cautiously measure out 50ml of hydrogen peroxide at 0% concentration in a measuring cylinder. 4) Pour this into the conical flask and securely seal the bung immediately within 2 seconds so that gas cannot escape and start the stop watch straight away. 5) Now gently swirl the conical flask at 60 seconds take the reading of the amount of oxygen that has collected in the gas syringe 6) Clean out the conical flask thoroughly and repeat experiment with 5, 10, 15 and 20% concentrations of Hydrogen peroxide. 7) Repeat each concentration 3 times so that you acquire more accurate results. 8) Calculate the average of each concentration and make graphs. 9) Then from your results work out the rate of each reaction. ...read more.

Conclusion

If the temperature increases too much for example over 50 degrees Celsius the active sites on the enzymes will change and become denatured. This will make the break down of hydrogen peroxide stop and will give me bad results. I also presume that when the concentration gets to high there will be a point where the enzymes will be working as fast as they can all active sites will be occupied at all times and as of this they will not be able to work any faster this means enzymes will reach there Vmax( maximum rate of a enzyme). I think my results should make a sloping graph to a point where it will not rise anymore. This is because of factors like enzymes reaching there Vmax. I predict my results will show a positive correlation as higher the concentration the faster the rate of the reaction as more oxygen will be produced. Results Gas produced after 1 minute (cm�) Concentration (%) Test 1 Test 2 Test 3 0% 0 0 0 5% 4 3 5 10% 21 26 32 15% 68 85 91 20% 71 82 84 Concentration Average Gas produced after 1 minute(cm�) 0% 0.0 5% 4.0 10% 26.3 15% 81.3 20% 79.0 Concentration Average rate of reaction (cm�/s) 0% 0.0 5% 0.06 10% 0.44 15% 1.355 20% 1.316 Rate= Average Volume of Gas Time Taken Biology Coursework Page 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Enzymes - investigate how the substrate concentration (H2O2) affects the activity of catalase on ...

    3 star(s)

    * Make sure goggles are worn at all times. * Make sure all laboratory surfaces are kept clean and clear throughout the experiment. * Make sure loose clothing is removed or tucked in. * Make sure long hair is tied back.

  2. Marked by a teacher

    Beetroot Practical Write up

    3 star(s)

    of the light transmittance Method Equipment: * Test tube rack * Boiling tubes * Graduated syringe * Measuring cylinders * Beakers * Ceramic tile * Cork borer * Razor blade * Thermometer * Forceps * Stop clock * Colourimeter Method: 1.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    tube at the start of the reaction; the other one is used to mix right amounts of other solutions. To make sure that the hydrogen peroxide doesn't make contact with the other reactants before the right apparatus is set up for the counting of the gas produced.

  2. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    Below is a table that clearly states how much of each substrate is needed to be weighed per experiment. The stated amount should be mixed to 15cm3 buffer solution. I will refer back to this table during my method/experiment. Substrate Mass (grams)

  1. Investigating The Effect of Substrate Concentration or Temperature On The Rate of Decomposition of ...

    Enzymes save the need for an increase in temperature in order to speed up reactions within living things. Minute quantities of an enzyme can accomplish at low temperatures what would require violent reactants and high temperatures by ordinary chemical means.

  2. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    delivery tube, so more water will be displaced from the conical flask. This will undoubtedly make all the results slightly inaccurate, but due to it happening to all the readings I take, the error will be cancelled out. I have also chosen to use precise apparatus as there is a smaller percentage error.

  1. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    Over periods of time the catalase can also decrease in activity in the same way as the hydrogen peroxide, due to oxidation(c). Prediction: I predict that as the enzyme concentration is increased then the rate of the substrate activity will also increase, because there will be more molecules of catalase,

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Increasing the enzyme concentration will increase the rate of reaction as this will increase the probability of successful collisions, but eventually it will level out because there is not an unlimited supply of substrate molecules. All my predictions are dependant that pH, pressure and temperature are constant and the amount/concentration of substrate molecules present remains unchanged.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work