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Investigating the effects of Sodium Hydroxide concentration on Catalase

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Investigating the effects of Sodium Hydroxide concentration on Catalase I will be investigating the effects of Sodium Hydroxide (NaOH) concentration on catalase, which is an enzyme. This would be done by use sheep liver as it contains catalase, and mixing it with different concentration of Sodium Hydroxide which is an alkaline, and then adding 10% of Hydrogen Peroxide to get the reaction going as catalase promotes the conversion of hydrogen peroxide. This is because catalase has a pH of 7 normally and where Sodium Hydroxide is an alkaline over time it will lose hydrogen ions to the solution and therefore decreases enzyme active. This investigation is to test the rate of reaction of catalase and Hydrogen Peroxide under different alkaline concentration of Sodium Hydroxide condition. Therefore I predict that as you increase the concentration of Sodium Hydroxide you will decrease the enzyme active, as this is because catalase in a alkaline solution loses hydrogen ions, which changes the produced of the chemical bonds of the enzyme molecule causing a change in the shape of the enzyme, cause the active of it to decrease, or in other words you denature the enzyme. This is likely to happen in the higher concentration of sodium hydroxide, where in the lower concentration due to it being less alkaline, more to the pH of catalase the lose of it hydrogen ions will be less or slower cause a increase in the rate of reaction. Therefore I also predict that my graph will look like this, having a fast reaction at the start but then slowing down till it stops. The apparatus that I would need to carry out this investigation would be, Gas syringe, Sheep Liver, Sodium Hydroxide (NaOH), Hydroxide Peroxide (2H2O2), Camp stand, Stop watch, Mortar and pestle, Conical flask, Tile, Scalpel, Scales, Distilled Water, Measuring tube and A bit of sand I believe that using these apparatus will give me the most relevant and relievable results to prove my prediction right. ...read more.


This would be down to the fact that catalase is an enzyme and will have it activity rate effect at different pH levels, as Sodium Hydroxide is an alkaline so the higher the concentration the more off a stronger alkaline it is, which in turn denature the enzyme meaning that it shape has changed and can no longer react with the substrate as it can no longer fit in the enzyme and therefore the quicker this happens the lower the rate of relation with be. Pattern that I have found in my graph is that they all seem to look like this This can be easily described why this has happened, but putting the graph into three sections A, B and C like so: Section A is the start of the reaction when you first add the liver to the Sodium Hydroxide then adding the Hydroxide Peroxide, the reaction is quick here no matter at what concentration the Sodium Hydroxide as the enzymes are reacting with the substrate faster then it can break then down, this part of the reaction can also be linked to the collision theory because of the fact that the reaction is only happening, and gas is being released because of the enzymes are colliding with the substrate, but as soon as all the enzymes have become denature the substrate can no longer fit into the enzyme so they can no longer react. However section A is the fastest bit of the reaction as a lot gas is being produced in a small amount of time. Section B is the part of the reaction where there are a few enzyme that have not be denature yet or there is only a few substrates to react with so the reaction slows down, though the reaction its happening it is at a much slower rate then before in section A, and this down to two thing, one being that only a few enzymes have not been denature and therefore the gas produce ...read more.


the enzymes.Could also use different materials that will react with catalase to see if that has any effect at all, you could use different tissues or different chemicals for this, like more and different alkaline as this is what I use in my investigation, as you could use different alkaline to see if they have a different effect or the some on catalase, as this will give a better sign to what the effects of different alkaline concentration have on catalase. I could also use more liver, use more then a gram of it to see what effect that has, which is likely to increase the amount of gas being produced, same as if I would to increase the amount of Sodium hydroxide and Hydroxide Peroxide would likely increase the gas produced or could have the opposite effect and decrease the amount of gas produced, as there is more of the alkaline substance and therefore it will be quicker at denaturing the enzymes and there will be less gas produced because the enzymes can no longer react with the substrate, as Sodium Hydroxide being an alkaline and catalase being a enzyme, over time, quicker in higher concentration of Sodium Hydroxide, catalyse will lose its hydrogen ions which changes the produced of the chemical bonds of the enzyme molecule causing a change in the shape of the enzyme or know as denaturing the enzyme, so the substrate can no longer fit into the enzyme and therefore can no longer react with it. Therefore I believe my investigation well apart from one set of result other then that, it went well, the experiment proved my prediction that as you increase the concentration of Sodium Hydroxide you decrease the amount of gas produced from the reaction, so therefore I believe that my results are good enough and relievable to prove it right and to show what happened in my investigation, so over all the investigation went well and my prediction was prove right. Paul Robinson Biology Coursework ...read more.

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