• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating The Rate Of Enzyme Activity With Varying Enzyme Concentration.

Extracts from this document...

Introduction

Investigating The Rate Of Enzyme Activity With Varying Enzyme Concentration. Introduction. In this experiment I hope to find out the effects of enzyme concentration on the rate of enzyme activity. I will do this by watching the varying levels of reactivity whilst experimenting with liver (which contains catalase) and hydrogen peroxide that will react to give off oxygen. Enzymes are biological catalysts that work according to the lock and key hypothesis. Enzymes contain an active site where the reaction of the two substrates takes place; the shape of this active site determines how effective the enzyme is. The higher the temperature the more collisions between the enzyme and substrates so the more reactions can take place. If the enzyme becomes too hot it will become denatured and the active site will be mutated and ineffective. Also, pH has an effect on the effectiveness of an enzyme. An enzyme needs to be in the right environment (pH) or it again becomes denatured and useless. Before I carried out the final experiment I did preliminary work in which I tried various ways of measuring the oxygen given off from the reaction. The first problem I came across was the matter of taking liver samples. ...read more.

Middle

Fig 3. For my first experiment I poured 10mls of the stock solution into the test tube and then lined this up with a ruler. I then added 5mls of the hydrogen peroxide and waited 30 seconds and then measured how far up the tube the froth had reached. This was recorded and the tubes were washed. Fig 4. What factors do you need to control or vary in this experiment? I need to control the amount of hydrogen peroxide in each experiment. This must always be the same, I have chosen to use 5mls every time. The amount of stock solution I use will always be 10mls, this is also a constant. I will vary the concentration of said stock solution, by diluting the original concentration as detailed above. This is an independent variable. I will also measure the amount of oxygen released by the reaction. This is a dependent variable as it is dependant on the concentration of catalase. I also need to maintain a constant temperature for all my experiments so this doesn't affect it. I will use the same size tubes and flasks each time so this has no effect on the reaction. ...read more.

Conclusion

How could the experiment be made more accurate? By using the equipment I did I made sure that all of the oxygen produced was definitely collected. Unfortunately, by using the equipment like I did, it meant that there was a chance that not all of the hydrogen peroxide would react. This was because when the flask was tipped not all of the hydrogen peroxide definitely came out of the test tube on the inside, I believe this was the cause of any inaccuracies in my results. To remedy this I would think it would be better to take a smaller test tube and attach it to the bottom of the flask, or use a small shelf attached to the side of the flask. This way it would make sure all the hydrogen peroxide mixed with all the stock solution. Fig 8. David Harrison 11A Fig 1. The two enzyme hypotheses. Fig 2. Preparing stock solutions. Fig 3. My final experiment. Fig 4. My preliminary experiment. Fig 5. My results. Fig 6. A catalyst and non-catalyst reaction, the alternate route. Fig 7. A catalyst and non-catalyst reaction, the lowered activation energy. Fig 8. How to make my experiment more accurate. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    0.2 315 + 305 + 321 = 941 � 3 = 314 314 0.4 224 + 236 +242 = 702 � 3 = 234 234 0.6 206 + 224 +216 = 646 � 3 = 215 215 0.8 187 + 177 + 194 = 558 � 3 = 186

  2. Marked by a teacher

    Factors effecting enzyme activity

    4 star(s)

    Assuming an adequate concentration of substrate is available, increasing enzyme concentration will increase the enzyme reaction rate. During my coursework, I will be investigating the effect of temperature on the rate of catalase activity. I am going to keep the pH and concentration of the enzyme constant.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    which I would use for my first concentration), I also filled 4 other test tubes each with 10 ml of buffer solution for the remaining concentrations * I put the 3 test tubes containing: Hydrogen Peroxide, potato in water, and copper sulphate solution, and a beaker containing water into the water bath to acclimatise for 3 minutes.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    As I increase the temperature the kinetic energy was gradually increasing. As the kinetic energy increases, the particles collide at a faster rate with a stronger force. This increases the rate of reaction because the particles have energy to react.

  1. Factors That Affect the Rate of an Enzyme Reaction.

    Whereas in solutions of low concentration reactant particles are far apart so there are only infrequent reactions, therefore the reaction is slow. I will control this variable by always using 10% yeast, and before measuring 0.5cm3 of yeast using a 1cm3 syringe I will always stir the yeast.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    the gas because the amount of gas collected can be recorded easily/accurately and it has a larger capacity to collect more gas. Accurate observation (to within a millimetre) is imperative when filling up the burette to the marking points and when taking a measurement, measurements must be read from the

  1. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    As the temperature in the room in which the experiments will be taking place is likely to fluctuate during the course of the reactions. Therefore, with the use of a water bath and a thermometer, continuous observation of the thermometer can be used to ensure a constant temperature can be maintained for every single experiment.

  2. THE EFFECT OF VARYING ENZYME CONCENTRATION ON THE ACTIVITY OF THE ENZYME CATALASE

    Besides the enzyme catalase has pH7 as its optimum pH. Stopwatch: I will use this to measure the amount of oxygen release per minute. The stopwatch is the most accurate measure of time as it can easily be stopped and restarted at my choice.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work