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Investigating the rate of reaction of enzymes at different temperatures in the clearing of photographic film.

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Investigating the rate of reaction of enzymes at different temperatures in the clearing of photographic film. Aim- My aim is to discover at what temperature enzymes work best and how the different temperatures affect the speed at which enzymes work to break down a certain substrate. Equipment- I will need for my experiment- * A number of water baths set at varying temperatures * Test tubes * Enzyme * Photographic film * Wire * Timer * Thermometer * Pipette Method- I am going to discover these things by placing photographic film into a water bath at a certain temperature and timing how long it takes for the enzyme to break the protein on the back of the photographic film down. This is how I plan to do it- * Set the water baths to the correct temperatures in ?C (35, 40, 45, 50, 55, 60, 65, 70, 75) * Attach a piece of photographic film 1cm by 2cm to the end of a piece of thin wire. * Fill a test tube with 20ml of the enzyme and place in the water bath. ...read more.


I will ensure these results are accurate by controlling the variables as far as possible. Safety As well as ensuring that the experiment is accurate and fair we must also look out for our safety and others during the experiment. There are a number of things that I could do to minimise the risks of injury. The water baths may be in some cases very hot. For this reason I must ensure that there is no risk of any water spilling and be very careful when near the water. When the test tube has been in the water for a long period of time it may become hot and therefore I must be careful when removing it from the water bath. I must handle the test tubes with care as if they are dropped or knocked they could shatter and cause injury. Fair testing- I will ensure that my investigation is a fair one by doing the following- * I will use the same amount of enzyme each time. * I will use the same area of photographic film each time. ...read more.


I think I will see a big change in the speed they work when I get to about 60 degrees. This is because temperatures above around 60 degrees usually damage enzymes. When I look at the graph I expect to see a sharp drop in their activity when the temperature gets too high. Earlier work- In the past I have done an experiment on enzymes and how the protease converts proteins into amino acids. I have shown how the catalyst can brake down the substrate. I have done another experiment as well as this where I placed the substrate and catalyst In a test tube in a water bath and tested a drop with iodine at set intervals. As we saw the colour change we could tell how much of the substrate was left and so could see how long it was taking the catalyst to break down the substrate. I have also done this experiment before in less depth. I did it with less intervals but I managed to discover some certain things from it. I could tell that the catalyst worked best at the higher temperatures and that it worked better at the temperatures close to body temperature (37 degrees). ...read more.

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