• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the rate of reaction of enzymes at different temperatures in the clearing of photographic film.

Extracts from this document...


Investigating the rate of reaction of enzymes at different temperatures in the clearing of photographic film. Aim- My aim is to discover at what temperature enzymes work best and how the different temperatures affect the speed at which enzymes work to break down a certain substrate. Equipment- I will need for my experiment- * A number of water baths set at varying temperatures * Test tubes * Enzyme * Photographic film * Wire * Timer * Thermometer * Pipette Method- I am going to discover these things by placing photographic film into a water bath at a certain temperature and timing how long it takes for the enzyme to break the protein on the back of the photographic film down. This is how I plan to do it- * Set the water baths to the correct temperatures in ?C (35, 40, 45, 50, 55, 60, 65, 70, 75) * Attach a piece of photographic film 1cm by 2cm to the end of a piece of thin wire. * Fill a test tube with 20ml of the enzyme and place in the water bath. ...read more.


I will ensure these results are accurate by controlling the variables as far as possible. Safety As well as ensuring that the experiment is accurate and fair we must also look out for our safety and others during the experiment. There are a number of things that I could do to minimise the risks of injury. The water baths may be in some cases very hot. For this reason I must ensure that there is no risk of any water spilling and be very careful when near the water. When the test tube has been in the water for a long period of time it may become hot and therefore I must be careful when removing it from the water bath. I must handle the test tubes with care as if they are dropped or knocked they could shatter and cause injury. Fair testing- I will ensure that my investigation is a fair one by doing the following- * I will use the same amount of enzyme each time. * I will use the same area of photographic film each time. ...read more.


I think I will see a big change in the speed they work when I get to about 60 degrees. This is because temperatures above around 60 degrees usually damage enzymes. When I look at the graph I expect to see a sharp drop in their activity when the temperature gets too high. Earlier work- In the past I have done an experiment on enzymes and how the protease converts proteins into amino acids. I have shown how the catalyst can brake down the substrate. I have done another experiment as well as this where I placed the substrate and catalyst In a test tube in a water bath and tested a drop with iodine at set intervals. As we saw the colour change we could tell how much of the substrate was left and so could see how long it was taking the catalyst to break down the substrate. I have also done this experiment before in less depth. I did it with less intervals but I managed to discover some certain things from it. I could tell that the catalyst worked best at the higher temperatures and that it worked better at the temperatures close to body temperature (37 degrees). ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    The substance that will be adsorbed onto the surface of the "active site" is called the substrate. Enzymes can be used both ways, in that they can be used to either make two products from a substrate or make a substrate from two products.

  2. Catalyse Investigation

    If there are sufficient substrate molecules to occupy all of the enzymes' active sites, the rate of reaction is unaffected by further increases in substrate concentration as the enzymes are unable to break down the greater quantity of substrate any quicker.

  1. What is a Catalyst?

    enzymes can work at very high pH, such as protease enzymes in animal stomachs, which have a maximum of pH 1. The pH affects the charge of the amino acids at the active site. The properties of the active site change and the substrate can no longer bind.

  2. A photographic company wants to recycle the plastic in exposed (and therefore unusable) photographic ...

    Temperature Temperature can alter the rate of reaction because enzymes work best at 40�C and if the temperature is lower then the molecules will move slower and therefore there will be less collisions of enzymes with substrates and the rate of reaction will be slower.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work