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Investigation into Haemolysis.

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Friday 3rd January 2003 BTEC National Diploma In Health Studies Roy Parker, Investigation into Haemolysis Hypothesis In this assignment I am going to be investigating the effects of osmosis on red blood cells. My hypothesis is that the solution with the highest concentration of water will be the 'strongest' colour. The reason why I think this is because with osmosis the more hypotonic the environment is around the cell the more likely it is too burst. Health and safety considerations The following is health and safety considerations; I used during my investigations- * Never point test tubes in the direction of a fellow student or at yourself. * It is your responsibility to ensure suitable protective clothing is worn during practical work (for example, a lab coat). * Protective eyewear must always be worn when you are working with any type of chemical. * The surrounding floor area and space on the work bench must be free of all work, coats and bags * The bench space is wiped after use. * Make sure the sink is free from any obstructions and is clean once you are finished. ...read more.


The reason for this is because water can enter and leave the cell easily. The direct result of this is that there was sediment at the bottom of the test tube. Test tube seven on the other hand has a higher concentration (than test tube six) and is hypertonic, which means there is a high concentration, so water leaves the cells (defibrinated blood cells), the cell shrunk and settled at the bottom of the test tube. Analysis of health and safety issues The following are explanations of why we certain safety precautions (listed on page 1) were followed. The reason why you never point test tubes in the direction of a fellow student or at yourself is in case of accident and potential harm to anyone who is a part of the experiment. There is the possibility of spillage of chemicals or the test tube is broke To ensure chemicals are not spilled onto clothing, a lab coat should always be worn preventing any irritation from chemicals. Protective eyewear must always be worn when you are working with any type of chemical, to prevent any chemical coming into contact with your eyes. ...read more.


Active transport differs, in that diffusion, because diffusion (and osmosis) are passive, whereas active transport only occurs in cells that contain adenosine triphosphate (ATP), which is why it is called 'active transport'. The energy that is in the form of ATP is generated by the mitochondria. Active transport occurs in many places in the body, e.g. to transport glucose quickly across the lining of the digestive system, it is also a carrier of protein. Evaluation I think that my experiment went extremely well. The hardest part about the experiment was measuring the amounts for the solutions, because I needed to get it as accurate as possible each time. This therefore means the results I received would be more exact, and make my experiment as accurate as possible. Except for accuracy, I don't think I had any other difficulties during the experiment. If I were to extend my experiment I would have 14 test tubes this time instead of 7, therefore having two lots of results for each strength solution. The direct result of this would mean that I would decrease the risk of error because I could combine my results and make an average, for each one, and increase the accuracy. ...read more.

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