• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the effect of substrate concentration on the activity of the enzyme catalase.

Extracts from this document...

Introduction

Investigation into the effect of substrate concentration on the activity of the enzyme catalase 2H2O2 ? 2H2O + O2 Hydrogen Peroxide ? Water + Oxygen The aim of this experiment is to investigate the affect of different concentrations of substrates on the rate of activity of the enzyme catalase. I will achieve this by diluting 10 volume hydrogen peroxide ten times to acquire 10 consecutively more dilute solutions. These will then be added to the same concentration of yeast suspension. The experiments will be performed at room temperature and oxygen will be collecting via a water bath and measuring cylinder. My predication is that as I increase the concentration of the substrate (hydrogen peroxide), the speed in which the reaction takes place, and amount of products produced will increase. This is because of the following reasons. Firstly, at a lower concentration of substrate, the amount of enzyme molecules present in the yeast would greatly outnumber the molecules of substrate being added. ...read more.

Middle

ice cream tub), clamp and stand and stopwatch Method 1. Measure out 50ml of yeast suspension into a conical flask 2. Measure out 100ml of 10 volume hydrogen peroxide into a separate container 3. Fill a tub with water 4. Completely immerse a measuring cylinder in the water making sure to release all air bubbles in the cylinder and keep underwater 5. Position the cylinder upright ensuring the open end remains underwater and clamp securely 6. Push the tubing from the conical flask into the cylinder 7. Add the hydrogen peroxide to the yeast suspension and place bung on conical flask whilst starting timer 8. Run experiment for 5 minutes taking readings every 20 seconds by noting the amount of gas at top of cylinder (at meniscus) When the experiment is repeated, the solution of hydrogen peroxide must be diluted. The following changes to the method for the next experiment would be: * Measure 90ml of 10 volume hydrogen peroxide in a separate container * Add 10ml of distilled water to solution Continue diluting solutions in this way until 10ml of distilled water is added to 90ml of hydrogen peroxide. ...read more.

Conclusion

Risk assessment 10 Volume Hydrogen Peroxide is an irritant - Wear goggles and handle carefully Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Results Table My results will be obtained and recorded in a table similar to the one below. Concentration is measured in 'volume', volume of gas in 'cm3' and time in 'seconds'. Volume of gas collected (cm3) after time in seconds Concentration (descending) 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 10 Volume 9 Volume 8 Volume 7 Volume 6 Volume 5 Volume 4 Volume 3 Volume 2 Volume 1 Volume Analysis of results Results would be analysed and then plotted on a line graph to compare the effects of the different concentrations. I will be looking for particular trends or similar curves on the graph to try to prove my prediction that as concentration increases so does rate of reaction. My main focus will be the initial gradient of the line and the varying amounts of oxygen produced with the first few minutes of the reaction. James Caullay 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    Also following safety techniques for handling chemicals e.g. enzymes such as washing hands after dealing with the enzymes, as they are water soluble and so are removed by water. When using the enzyme, ensure lid is replaced because if liquid enzyme dries up the dried enzyme could be inhaled and may provoke asthma or hayfever.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    Apparatus: Apparatus nomenclature Use Reasoning for choice Black marker To mark the test tubes with appropriate names To distinguish the various solutions, since at times some of them have same colours. Test tubes To contain the solutions, before the reaction, or during the course of it.

  1. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    Another safety risk is the glass test tubes that will be used. If broken, then sharp pieces of broken glass could result. This could cause cuts to the skin if not dealt with in a safe way. Open wounds could then get chemicals in which would also be hazardous.

  2. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    saccharomyces cerevisiae solution, I am more likely to forget or even mix the two up. So I think that 15cm3 of both the saccharomyces cerevisiae and substrate solution is a suitable volume that will both fit into the conical flask, when added together.

  1. for this experiment my main aim is to investigate the effect of temperature on ...

    Kettle: allows me to boil water so I can use it to acquire the correct temperature required for the experiment (by mixing it with cold water) Labels: allows me to distinguish different substances more easily if their containers are labeled.

  2. Catalyse Investigation

    By calculating the rate of reaction instead of merely using the time readings, the quicker reactions will be represented as a greater value for the rate of reaction rather than a small time value. This makes the graph more clear and easier to analyse.

  1. Amylase Investigation

    3 = 286.6 Average for 300C - 210 270 220 700 700 ? 3 = 233.3 Average for 350C - 165 165 170 500 500 ? 3 = 166.6 Average for 400C - 150 150 160 460 460 ? 3 = 153.3 Average for 450C - 170 200 190 560 560 ?

  2. An Investigation to determine the effect of Substrate Concentration on the Enzyme Catalase

    Many bonds that hold an enzyme's tertiary structure together are bonds between positively and negatively charged R groups. When pH changes there's change in the concentration of Hydrogen ions which cancels out the charge on these R groups so the tertiary structure changes, which means that active site lose their specific shape and substrate molecules do not fit.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work