• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the effect of substrate concentration on the activity of the enzyme catalase.

Extracts from this document...


Investigation into the effect of substrate concentration on the activity of the enzyme catalase 2H2O2 ? 2H2O + O2 Hydrogen Peroxide ? Water + Oxygen The aim of this experiment is to investigate the affect of different concentrations of substrates on the rate of activity of the enzyme catalase. I will achieve this by diluting 10 volume hydrogen peroxide ten times to acquire 10 consecutively more dilute solutions. These will then be added to the same concentration of yeast suspension. The experiments will be performed at room temperature and oxygen will be collecting via a water bath and measuring cylinder. My predication is that as I increase the concentration of the substrate (hydrogen peroxide), the speed in which the reaction takes place, and amount of products produced will increase. This is because of the following reasons. Firstly, at a lower concentration of substrate, the amount of enzyme molecules present in the yeast would greatly outnumber the molecules of substrate being added. ...read more.


ice cream tub), clamp and stand and stopwatch Method 1. Measure out 50ml of yeast suspension into a conical flask 2. Measure out 100ml of 10 volume hydrogen peroxide into a separate container 3. Fill a tub with water 4. Completely immerse a measuring cylinder in the water making sure to release all air bubbles in the cylinder and keep underwater 5. Position the cylinder upright ensuring the open end remains underwater and clamp securely 6. Push the tubing from the conical flask into the cylinder 7. Add the hydrogen peroxide to the yeast suspension and place bung on conical flask whilst starting timer 8. Run experiment for 5 minutes taking readings every 20 seconds by noting the amount of gas at top of cylinder (at meniscus) When the experiment is repeated, the solution of hydrogen peroxide must be diluted. The following changes to the method for the next experiment would be: * Measure 90ml of 10 volume hydrogen peroxide in a separate container * Add 10ml of distilled water to solution Continue diluting solutions in this way until 10ml of distilled water is added to 90ml of hydrogen peroxide. ...read more.


Risk assessment 10 Volume Hydrogen Peroxide is an irritant - Wear goggles and handle carefully Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Results Table My results will be obtained and recorded in a table similar to the one below. Concentration is measured in 'volume', volume of gas in 'cm3' and time in 'seconds'. Volume of gas collected (cm3) after time in seconds Concentration (descending) 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 10 Volume 9 Volume 8 Volume 7 Volume 6 Volume 5 Volume 4 Volume 3 Volume 2 Volume 1 Volume Analysis of results Results would be analysed and then plotted on a line graph to compare the effects of the different concentrations. I will be looking for particular trends or similar curves on the graph to try to prove my prediction that as concentration increases so does rate of reaction. My main focus will be the initial gradient of the line and the varying amounts of oxygen produced with the first few minutes of the reaction. James Caullay 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    Only one piece of each apparatus item will be needed as one experiment will be carried out at a time and the same apparatus will be used for repeating results. Planned Method 1. Wall paper paste is a good source of cellulose, the substrate in this experiment.

  2. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    The yeast being used has little safety danger as it has a low safety risk. Ethical Considerations The catalase enzyme can be obtained from many different sources. For example liver is a good source of catalase. However, because some people may object to the use of animal products in experiments,

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    This depended on the amount of time we had. If we could have done each experiment for something close to 5 or 6 minutes, and still do three replicate results, we might have been able to observe the slowing down of the reactions and a more obvious curve, rather than a borderline straight-line.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    The substrate molecules are 'queuing up' for an active site to become vacant. The enzyme as substrate concentration increases, the initial rate of reaction also increases. This is again obvious, as the more substrate molecules there are around, there more often an enzyme's active site can bind with one.

  1. for this experiment my main aim is to investigate the effect of temperature on ...

    Below is my prediction graph: My prediction graph shows that as temperature increases from 10oC - 40oC the rate of reaction also increases (maximum at 40oC), and rate of reaction will start to decrease after 40oC; this is an evidence that as temperature increases past the optimum temperature the enzyme starts to denature therefore its activity is also affected.


    The vibration increases as does temperature. The fast vibrations cause weak hydrogen bonds that maintain the 3D tertiary structure of the enzyme, to break causing the shape of the enzyme to be distorted. As a result the shape of the active site is also disrupted.

  1. Catalyse Investigation

    The reaction will increase with increasing enzyme concentration when molecules of hydrogen peroxide are freely available. However, when molecules of the substrate are in short supply, the increase in rate of reaction is limited and will have little effect. Variables - In this investigation, the variables that affect the activity

  2. An Investigation on the Effect of Enzyme Concentration on rate of hydrogen peroxide breakdown.

    oxygen produced was noted The results: the 2cm� celery extract acted as 100% celery extract. i.e. 50% celery extract would be 1 cm� celery extract and 1ml distilled water. The results are recorded in the table below: Percentage conc. of celery extract (%)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work