• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the effect of substrate concentration on the activity of the enzyme catalase.

Extracts from this document...

Introduction

Investigation into the effect of substrate concentration on the activity of the enzyme catalase 2H2O2 ? 2H2O + O2 Hydrogen Peroxide ? Water + Oxygen The aim of this experiment is to investigate the affect of different concentrations of substrates on the rate of activity of the enzyme catalase. I will achieve this by diluting 10 volume hydrogen peroxide ten times to acquire 10 consecutively more dilute solutions. These will then be added to the same concentration of yeast suspension. The experiments will be performed at room temperature and oxygen will be collecting via a water bath and measuring cylinder. My predication is that as I increase the concentration of the substrate (hydrogen peroxide), the speed in which the reaction takes place, and amount of products produced will increase. This is because of the following reasons. Firstly, at a lower concentration of substrate, the amount of enzyme molecules present in the yeast would greatly outnumber the molecules of substrate being added. ...read more.

Middle

ice cream tub), clamp and stand and stopwatch Method 1. Measure out 50ml of yeast suspension into a conical flask 2. Measure out 100ml of 10 volume hydrogen peroxide into a separate container 3. Fill a tub with water 4. Completely immerse a measuring cylinder in the water making sure to release all air bubbles in the cylinder and keep underwater 5. Position the cylinder upright ensuring the open end remains underwater and clamp securely 6. Push the tubing from the conical flask into the cylinder 7. Add the hydrogen peroxide to the yeast suspension and place bung on conical flask whilst starting timer 8. Run experiment for 5 minutes taking readings every 20 seconds by noting the amount of gas at top of cylinder (at meniscus) When the experiment is repeated, the solution of hydrogen peroxide must be diluted. The following changes to the method for the next experiment would be: * Measure 90ml of 10 volume hydrogen peroxide in a separate container * Add 10ml of distilled water to solution Continue diluting solutions in this way until 10ml of distilled water is added to 90ml of hydrogen peroxide. ...read more.

Conclusion

Risk assessment 10 Volume Hydrogen Peroxide is an irritant - Wear goggles and handle carefully Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Results Table My results will be obtained and recorded in a table similar to the one below. Concentration is measured in 'volume', volume of gas in 'cm3' and time in 'seconds'. Volume of gas collected (cm3) after time in seconds Concentration (descending) 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 10 Volume 9 Volume 8 Volume 7 Volume 6 Volume 5 Volume 4 Volume 3 Volume 2 Volume 1 Volume Analysis of results Results would be analysed and then plotted on a line graph to compare the effects of the different concentrations. I will be looking for particular trends or similar curves on the graph to try to prove my prediction that as concentration increases so does rate of reaction. My main focus will be the initial gradient of the line and the varying amounts of oxygen produced with the first few minutes of the reaction. James Caullay 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of enzyme concentration on the activity of cellulase.

    5 star(s)

    Then to try to find the optimum or best substrate concentration that will give a viscosity that will flow through the syringe barrel within the time constraints (maximum 10 minutes for the substrate to flow through)

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    14.0 16.0 13.0 18.0 300 16.0 18.0 15.0 20.0 360 17.0 19.0 16.5 22.0 420 19.0 19.5 18.0 24.0 480 20.0 20.0 19.5 25.0 540 21.0 22.0 22.0 26.0 600 22.5 23.0 24.5 27.0 720 23.0 24.0 25.0 29.0 780 24.0 25.0 28.0 33.6 Key: Surface area 1= 1 potato

  1. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    will be washed under running, cold water for at least 10 minutes, not using soap. Any contaminated clothes will be removed and in a very serious incident then transport to hospital will be arranged where information about the chemical and prior treatment will be passed on.

  2. WHAT EFFECT DOES SUBSTRATE HAVE ON THE RATE OF RESPIRATION IN SACCHAROMYCES CEREVISIAE?

    So a 1M substrate solution is a workable and practical concentration for my experiment. CALCULATIONS The two formulas below is what I shall use to calculate the mass of each substrate I am required to weigh to ensure that when I dissolve it in 15cm3, the concentration is constant at

  1. Amylase Investigation

    time the enzyme denatured quickly and the third time the starch disappeared after 6 minutes (400 seconds) which was roughly twice as much as the first experiment. At 60oC, the second time the experiment was conducted at this temperature, the starch took twice as long (6minutes or 360 seconds)

  2. Catalyse Investigation

    I began with an enzyme concentration of 15 potato discs rather than a lower quantity to ensure that the apparatus was working correctly. Vol. pH buffer (ml) Vol. Hydrogen peroxide (ml) No.Potato discs 5 5 5 5 5 10 5 5 15 5 5 20 5 5 25 5 5

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    to 1 decimal 13.4 15.6 18.1 11.5 13.5 16.1 Point 50% 25% Volume of Oxygen Gas produced (cm3) Anwar 6.5 9.5 11.5 0 0 0 Babu 10.4 12.8 14.4 9.8 12.8 14.1 Da Costa 8.8 10.1 12.2 6.9 9.1 11.3 Francis 9 11.5 13.8 3.7 4.6 5.9 Hussain 11 12.5

  2. An Investigation to determine the effect of Substrate Concentration on the Enzyme Catalase

    TABLE 1 (A TABLE SHOWING THE FACTORS AFFECTING ENZYME ACTIVITY) A Graph to Demonstrate the Explanation Explanation of how the Specific Factor affects the Enzyme's Activity Factor affecting Enzyme Activity Temperature At M there is a slow reaction because molecules of enzyme and substrate have little kinetic energy and rarely collide.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work