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Investigation into the effect of substrate concentration on the activity of the enzyme catalase.

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Introduction

Investigation into the effect of substrate concentration on the activity of the enzyme catalase 2H2O2 ? 2H2O + O2 Hydrogen Peroxide ? Water + Oxygen The aim of this experiment is to investigate the affect of different concentrations of substrates on the rate of activity of the enzyme catalase. I will achieve this by diluting 10 volume hydrogen peroxide ten times to acquire 10 consecutively more dilute solutions. These will then be added to the same concentration of yeast suspension. The experiments will be performed at room temperature and oxygen will be collecting via a water bath and measuring cylinder. My predication is that as I increase the concentration of the substrate (hydrogen peroxide), the speed in which the reaction takes place, and amount of products produced will increase. This is because of the following reasons. Firstly, at a lower concentration of substrate, the amount of enzyme molecules present in the yeast would greatly outnumber the molecules of substrate being added. ...read more.

Middle

ice cream tub), clamp and stand and stopwatch Method 1. Measure out 50ml of yeast suspension into a conical flask 2. Measure out 100ml of 10 volume hydrogen peroxide into a separate container 3. Fill a tub with water 4. Completely immerse a measuring cylinder in the water making sure to release all air bubbles in the cylinder and keep underwater 5. Position the cylinder upright ensuring the open end remains underwater and clamp securely 6. Push the tubing from the conical flask into the cylinder 7. Add the hydrogen peroxide to the yeast suspension and place bung on conical flask whilst starting timer 8. Run experiment for 5 minutes taking readings every 20 seconds by noting the amount of gas at top of cylinder (at meniscus) When the experiment is repeated, the solution of hydrogen peroxide must be diluted. The following changes to the method for the next experiment would be: * Measure 90ml of 10 volume hydrogen peroxide in a separate container * Add 10ml of distilled water to solution Continue diluting solutions in this way until 10ml of distilled water is added to 90ml of hydrogen peroxide. ...read more.

Conclusion

Risk assessment 10 Volume Hydrogen Peroxide is an irritant - Wear goggles and handle carefully Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Results Table My results will be obtained and recorded in a table similar to the one below. Concentration is measured in 'volume', volume of gas in 'cm3' and time in 'seconds'. Volume of gas collected (cm3) after time in seconds Concentration (descending) 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 10 Volume 9 Volume 8 Volume 7 Volume 6 Volume 5 Volume 4 Volume 3 Volume 2 Volume 1 Volume Analysis of results Results would be analysed and then plotted on a line graph to compare the effects of the different concentrations. I will be looking for particular trends or similar curves on the graph to try to prove my prediction that as concentration increases so does rate of reaction. My main focus will be the initial gradient of the line and the varying amounts of oxygen produced with the first few minutes of the reaction. James Caullay 1 ...read more.

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