• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the effect of temperature on the activity of the enzyme urease.

Extracts from this document...


Investigation into the effect of temperature on the activity of the enzyme urease Urea + Urease ? Ammonium Carbonate The aim of this experiment is to investigate the affect of different temperatures on the rate of activity of the enzyme urease. I will achieve this by heating 5 solutions to different temperatures. They will be heated in a water bath and left for approximately 40 minutes. After this time, acid will be added to neutralize the solution and the activity of the enzyme will be measured via the volume of acid added. My predication is that as I increase the temperature of the solutions, the speed in which the reaction takes place, and amount of products produced will increase. However, I predict that at a high temperature the enzymes will denature and activity will cease. This is because of the following reasons. Firstly, at a high temperature, molecules are given more kinetic energy. ...read more.


Also, at around 60�C, the yeast ceased to be active, suggesting that denaturing of the enzymes occurred. Equipment Six test tubes, measuring cylinder, water baths, glass rod, mortar and pestle, 250ml beaker, stopwatch Method Preparing the urease solution 1. Crush one tablet of urease into a fine powder using the mortar and pestle 2. Add the powder to 3cm3 of distilled water in a test tube 3. Stir with a glass rod, and allow any insoluble substances to settle and then pour mixture off into clean test tube Heating the mixtures 1. Mix 1cm3 of urease solution with 10cm3 of 1% urea solution and pour into a test tube 2. Place the solution into a water bath at 20�C Measuring the activity 1. Add 2 or three drops of screened methyl orange indicator to the solution 2. Add 0.05 mol dm3 sulphuric acid by using a burette 3. Continually 'swirl' the solution 4. ...read more.


This however may need to be altered if tests at 60�C show some activity. Risk assessment Urea - Toxic Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Sulphuric Acid - Corrosive / Irritant Results Table My results will be obtained and recorded in a table similar to the one below. Temperature measured in '�C' and volume of sulphuric acid in 'cm3. Temperature (�C) Volume of sulphuric acid (cm3) 20�C 30�C 40�C 50�C 60�C Analysis of results Results would be analysed and then plotted on a line graph to compare the effects of the different concentrations. I will be looking for particular trends or similar curves on the graph to try to prove my prediction that as temperature increases so does rate of activity and to prove my theory of the 'cut off' temperatures. My main focus will be the temperature at which the activity at its slowest and quickest. High amounts of sulphuric acid will be interpreted as high activity, as more amounts of ammonium carbonate will be produced. Low amounts of acid will be the opposite of this. James Caullay 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

**Plan only. This is a very minimal plan which would be awarded little credit under current marking guidelines.
To improve
It does not inspire confidence in a plan when the first vital formula is incorrect. The candidate must check these carefully before submission.
There is some discussion of the background biological theory to this investigation but there is a lack of suitably selected references to assist in the planning and execution of the investigation. These should be beyond those most readily to hand textbooks and should be used effectively to provide some context for the investigation. The references should be cited appropriately.

A pilot experiment was carried out. The methodology for this as well as the results should be included as in some mark schemes this is expected and only the lower tier of marks is awarded if evidence of this is not included.

There needs to be more discussion on the key variables in the experiment. This is a key part of experimental planning. This can often best be achieved by constructing a table with the name of the variable in the first column, how it is to be controlled in the second column and the effect on the experiment if tit is not controlled well in the third column.
The method lacks some key information such as the strength and source of the urease.
The risk assessment would be considered unsatisfactory under some the newest marking guidelines since it lacks a lot of key information.
The headings for the results table need all units to be present and it would be expected that a candidate would plan for some replicates and a numerical or statistical method of analyzing the results.

Marked by teacher Stevie Fleming 22/08/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Effect of temperature on the enzyme pectinase in fruit juice production.

    3 star(s)

    This enzyme would behave in a more predictable manner. 6. The experiment should be repeated more than twice to obtain more results and a better average. It should be repeated at least 3 times to obtain more reliable results, minimise errors and obtain more accurate average.

  2. Effects of Copper Sulphate on the Activity of Catalase

    3 3 20 5 6 6 4 5 5 3.5 3.5 3.5 3 3 3 3 3 3 30 6.5 6.7 7.2 5.1 5.1 5.1 4.6 4.5 4.7 3 4 4 3 3 4 40 7 8 7 6.5 6.5 6.5 4.9 5 4.8 4 4 5 3.7 3.8 3.9

  1. An experiment to investigate the effect of temperature on the action of the enzyme ...

    Everything was fine until we had to add the lipase. I had to somehow manage to put lipase into two test tubes without it going everywhere. This was quite unsuccessful and may have contributed to the anomalous results I obtained.

  2. The effect of enzyme concentrations on the reaction time of Urease active meal.

    Hydrogen bonding holds molecules together more strongly than other types of intermolecular forces but is weaker than covalent bonding. For hydrogen bonding to occur a hydrogen atom needs to be attached to a highly electronegative atom that needs to be small, and with at least one lone pair of electrons for the Hydrogen atom to interact with.

  1. The Effect of Concentration on Pectinase Using Apple

    Pilot Experiment: In order to make sure my outline method is sufficient enough to produce a range of results and that the experiment can be carried out safely and accurately, a pilot experiment was done. During this experiment it was hoped that any errors or improvements to the method were found out.

  2. An experiment to investigate the effect of chloride ion concentration on the activity of ...

    Seek medical attention if irritation develops or persists Glassware Precautions: As glassware will be used, the up most care to prevent breakage will be taken. This includes never placing cylindrical glassware on the bench where it is able to roll off.

  1. Investigating The Activity Of The Enzyme Lipase On Milk

    When these forces are disturbed and changed the active site becomes altered in its ability to accommodate the substrate molecules it was intended to catalase. Most enzymes in a human body shut down beyond certain temperatures. This can happen if body temperature gets too low (hypothermia), or too high (overheating).

  2. The investigation to find the effect of glucose concentration on fermentation of yeast.

    Most enzymes have an optimum pH of around 7 and an optimum temperature of around 30 C to 40C (e.g. the optimum temperature of human enzymes is about 37C). They are very sensitive to pH and temperature changes. Temperature change can break the hydrogen bonds and pH change can alter

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work