• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation of the breakdown of Hydrogen Peroxide by the Enzyme Catalase.

Extracts from this document...


INVESTIGATION OF THE BREAKDOWN OF HYDROGEN PEROXIDE BY THE ENZYME CATALASE AIM: To investigate the breakdown of hydrogen peroxide by the enzyme catalase. PREDICTION I predict that increasing the temperature will increase the rate of oxygen given off. After the optimum point (peak point) the enzyme's shape becomes altered due to high temperatures. The enzyme is said to be denatured and the rate of oxygen will fall rapidly. Summarising my prediction gives. The temperature is proportional to the rate until it reaches it's optimum point were the rate falls. I also predict that the optimum point of the enzyme will be between 50-60 degrees. If I only increase the temperature of the substrate I predict that even more oxygen would evolve this is because when the substrate is at a high temperature the enzyme would not be hot and so it would not be denatured. The enzyme would react quickly to the substrate until it becomes hot and denatured. This would probably last a few seconds, but it would vary from different temperatures. If I only increase the temperature of the enzyme. I predict that less oxygen would evolve because when the enzyme is hot it becomes denatured unlike the substrate, which can take high temperatures. ...read more.


2 27.0 57.0 74.0 86.0 97.0 105.0 AVERAGRE 29.0 60.0 76.0 90.0 100.0 107.0 70 degrees 30 60 90 120 150 180 VOL .1 23.0 48.0 60.0 76.0 85.0 96.0 VOL. 2 29.0 54.0 66.0 81.0 93.0 102.0 AVERAGRE 26.0 51.0 63.0 79.0 89.0 99.0 80 degrees 30 60 90 120 150 180 VOL .1 19.0 39.0 50.0 58.0 70.0 79.0 VOL. 2 25.0 43.0 54.0 64.0 76.0 83.0 AVERAGRE 22.0 41.0 52.0 61.0 73.0 81.0 Increasing the temperature of only the substrate. Time/seconds 30 60 90 120 150 180 VOL .1 33.0 82.0 93.0 126.0 118.0 126.0 VOL. 2 41.0 69.0 90.0 103.0 122.0 134.0 AVERAGRE 37.0 67.0 86.0 98.0 119.0 130.0 VOL .1 31.0 37.0 40.0 48.0 52.0 57.0 VOL. 2 25.0 29.0 36.0 40.0 48.0 51.0 AVERAGRE 28.0 33.0 38.0 44.0 50.0 54.0 VOL .1 33.0 42.0 53.0 58.0 63.0 71.0 VOL. 2 41.0 50.0 59.0 63.0 72.0 80.0 AVERAGRE 37.0 46.0 57.0 60.0 69.0 75.0 VOL .1 28.0 43.0 56.0 67.0 78.0 89.0 VOL. 2 36.0 63.0 72.0 80.0 86.0 97.0 AVERAGRE 32.0 58.0 69.0 77.0 82.0 93.0 VOL .1 57.0 86.0 98.0 101.0 105.0 115.0 VOL. 2 50.0 76.0 84.0 93.0 97.0 103.0 AVERAGRE 54.0 80.0 92.0 97.0 103.0 111.0 VOL .1 34.0 43.0 56.0 61.0 69.0 73.0 VOL. ...read more.


Non revisable inhibitors They leave the enzyme permanently damaged and so unable to carry out its catalytic function. The bonds maintain the shape of the enzyme molecule. Once broken the enzyme molecule structure becomes irreversibly altered with the permanent loss of its catalytic properties Another experiment, which I could do, is to measure the volume of the mixture of enzyme and hydrogen peroxide before and after the experiment. This experiment could be done by: * Set up the experiment as shown. * Measuring 2ml of catalase and 20ml of hydrogen peroxide. * Pour them in the conical flask and let them react for three minutes. * Make show that in each experiment that the concentration is the same, the temperature is the same and the time is the same. * After the experiment pour the mixture into a measuring cylinder and record the results and find the difference between the volume before and after the experiment. * Try doing the experiment with different temperatures and seeing what happens to the volume of the mixture after the experiment. * You could also do this experiment by changing the concentration. * But make shore that you change one variable each time. 180 142 147 92 120 112 107 99 81 180 130 54 75 93 111 70 88 96 180 138 137 85 116 118 106 97 80 ?? ?? ?? ?? IBRAHIM ELGAYER ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    How does the concentration of enzymes affect the breakdown of starch by a-amylase in ...

    4 star(s)

    of the enzyme solution will, as I have said before, not be the limiting factor. I do not, however, know this as a fact, so I cannot come to any valid conclusion on whether the graph levels off or whether the graph keeps on increasing.

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    However the accuracy of the results could have been improved further by repeating the experiment again. The different enzyme concentrations were all kept under the same conditions, as was the substrate. I insured the Hydrogen peroxide and celery came from the same place so the results could be as reliable as possible.

  1. Catalyse Investigation

    Once the apparatus had been reset and any air bubbles in the manometer fluid removed, a second and later third reading was taken by re-closing the clamp and measuring the time taken for sufficient gas to be released from the reaction to force the manometer fluid back up to the top mark.

  2. Amylase Investigation

    Using the control dimple, keep testing the solution every 30 seconds till there is a negative result and the colour of the solution is no longer black. 9. Repeat steps 3 - 8 raising the temperature of the water

  1. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    The two then bind and form an enzyme-substrate complex. It is held in place using temporary bonds which form between the R groups of the enzyme's amino acid and the substrate. These bonds are weak and thus are not covalent The specific shape of the enzyme can be understood by the lock and key diagram.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    are getting faster, so therefore if the reactions are occurring faster this suggests that there must be a lot of collisions between the catalase and the hydrogen peroxide particles. As referring to the graph I can say that as the temperature increases from 10?c to 60?c, the rate at which the reaction the reaction occurs also increases.

  1. This is an experiment to show how different concentration of celery tissue enzyme, catalase ...

    For living organisms this is body temperature. If it gets too hot, however, an enzyme may no longer be able to help reactions occur. This is because at higher temperatures the protein molecule that is the enzyme may start to 'unravel' as its bonds break, and its surface may no longer be the correct shape for the substrates to fit.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Chemical spillages can be wiped with a damp cloth and dried with a paper towel. Safety Goggles should be worn to prevent eye-contact with the solutions and apparatus. Any accident concerning the eye should be replied with flooding the eye with water and immediately reported to the supervisor.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work