• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation of the breakdown of Hydrogen Peroxide by the Enzyme Catalase.

Extracts from this document...

Introduction

INVESTIGATION OF THE BREAKDOWN OF HYDROGEN PEROXIDE BY THE ENZYME CATALASE AIM: To investigate the breakdown of hydrogen peroxide by the enzyme catalase. PREDICTION I predict that increasing the temperature will increase the rate of oxygen given off. After the optimum point (peak point) the enzyme's shape becomes altered due to high temperatures. The enzyme is said to be denatured and the rate of oxygen will fall rapidly. Summarising my prediction gives. The temperature is proportional to the rate until it reaches it's optimum point were the rate falls. I also predict that the optimum point of the enzyme will be between 50-60 degrees. If I only increase the temperature of the substrate I predict that even more oxygen would evolve this is because when the substrate is at a high temperature the enzyme would not be hot and so it would not be denatured. The enzyme would react quickly to the substrate until it becomes hot and denatured. This would probably last a few seconds, but it would vary from different temperatures. If I only increase the temperature of the enzyme. I predict that less oxygen would evolve because when the enzyme is hot it becomes denatured unlike the substrate, which can take high temperatures. ...read more.

Middle

2 27.0 57.0 74.0 86.0 97.0 105.0 AVERAGRE 29.0 60.0 76.0 90.0 100.0 107.0 70 degrees 30 60 90 120 150 180 VOL .1 23.0 48.0 60.0 76.0 85.0 96.0 VOL. 2 29.0 54.0 66.0 81.0 93.0 102.0 AVERAGRE 26.0 51.0 63.0 79.0 89.0 99.0 80 degrees 30 60 90 120 150 180 VOL .1 19.0 39.0 50.0 58.0 70.0 79.0 VOL. 2 25.0 43.0 54.0 64.0 76.0 83.0 AVERAGRE 22.0 41.0 52.0 61.0 73.0 81.0 Increasing the temperature of only the substrate. Time/seconds 30 60 90 120 150 180 VOL .1 33.0 82.0 93.0 126.0 118.0 126.0 VOL. 2 41.0 69.0 90.0 103.0 122.0 134.0 AVERAGRE 37.0 67.0 86.0 98.0 119.0 130.0 VOL .1 31.0 37.0 40.0 48.0 52.0 57.0 VOL. 2 25.0 29.0 36.0 40.0 48.0 51.0 AVERAGRE 28.0 33.0 38.0 44.0 50.0 54.0 VOL .1 33.0 42.0 53.0 58.0 63.0 71.0 VOL. 2 41.0 50.0 59.0 63.0 72.0 80.0 AVERAGRE 37.0 46.0 57.0 60.0 69.0 75.0 VOL .1 28.0 43.0 56.0 67.0 78.0 89.0 VOL. 2 36.0 63.0 72.0 80.0 86.0 97.0 AVERAGRE 32.0 58.0 69.0 77.0 82.0 93.0 VOL .1 57.0 86.0 98.0 101.0 105.0 115.0 VOL. 2 50.0 76.0 84.0 93.0 97.0 103.0 AVERAGRE 54.0 80.0 92.0 97.0 103.0 111.0 VOL .1 34.0 43.0 56.0 61.0 69.0 73.0 VOL. ...read more.

Conclusion

Non revisable inhibitors They leave the enzyme permanently damaged and so unable to carry out its catalytic function. The bonds maintain the shape of the enzyme molecule. Once broken the enzyme molecule structure becomes irreversibly altered with the permanent loss of its catalytic properties Another experiment, which I could do, is to measure the volume of the mixture of enzyme and hydrogen peroxide before and after the experiment. This experiment could be done by: * Set up the experiment as shown. * Measuring 2ml of catalase and 20ml of hydrogen peroxide. * Pour them in the conical flask and let them react for three minutes. * Make show that in each experiment that the concentration is the same, the temperature is the same and the time is the same. * After the experiment pour the mixture into a measuring cylinder and record the results and find the difference between the volume before and after the experiment. * Try doing the experiment with different temperatures and seeing what happens to the volume of the mixture after the experiment. * You could also do this experiment by changing the concentration. * But make shore that you change one variable each time. 180 142 147 92 120 112 107 99 81 180 130 54 75 93 111 70 88 96 180 138 137 85 116 118 106 97 80 ?? ?? ?? ?? IBRAHIM ELGAYER ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    How does the concentration of enzymes affect the breakdown of starch by a-amylase in ...

    4 star(s)

    The average result will therefore not be as accurate as the averages with the shorter range bars. Looking at the difference in diameter of the clearance rings for each concentration: Concentration difference (%) Distance by which clearance rings have increased (mm)

  2. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    On the following page I have drawn a graph to show the data that I collected, from this graph I will be able to see the data more clearly in comparison to each other. I shall also draw a graph to show the initial rate of reaction for each concentration that will follow the other graph.

  1. Catalyse Investigation

    meniscus was lined up properly with the gradations and there were no air bubbles in the syringe. A pH buffer was added to the boiling tubes to maintain the pH at a constant level so that changes in pH as a result of the reaction would not affect the activity of the enzyme and disrupt the results.

  2. Amylase Investigation

    * Finally, test until about 700C rather than 60-700C as the preliminary test showed that the enzyme activity still continued slightly at 700C. Final Method 1. Warm up 200cm3 of water in a beaker to about 200C. This will act as a water bath for the test tubes during the experiment.

  1. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    This structure is brought about by the long amino acid chains coiling up on themselves. Hydrogen bonds then form between the -CO groups of one amino acid and the -NH group of another, which hold this shape in place. This is called an ?- helix and is the secondary structure.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    * I cut out identical filter paper discs using a hole punch * I obtain different strength of the catalase solution in a beaker * I used forceps to dip the filter paper in the catalase solution. * I then lowered the filter paper flat onto the top of the

  1. An Investigation to determine the effect of Substrate Concentration on the Enzyme Catalase

    The rate at which the reaction occurs entirely depends on the number of enzymes present, and the rate at which they convert the H2O2 substrate into its products, i.e. water and Oxygen, and release them, and then bind with another substrate molecule.

  2. An investigation to see how different concentrations of aspirin affects hydrogen peroxide breakdown by ...

    If smashed: Clean up immediately, disposing of glass in a suitable way. If cut by glass: Clean wound, and treat depending on seriousness. Seek assistance. Aspirin Harmful. An irritant to the stomach. I will avoid ingestion. I will keep the substance in a labelled container.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work