• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation of the effect of temperature on the enzyme controlled reaction

Extracts from this document...


Investigation of the effect of temperature on the enzyme controlled reaction Background The change of temperature will affect the rate of a reaction. This is because that for an amount of reactants, increase temperature will give it more heat energy and the molecules in it will have greater kinetic energy. For the mass of the molecules are not changed, these molecules will move with greater speed. (KE=1/2mv^) The reaction occurs when the reactant molecules collide with each other. The substrate molecules and enzyme molecules with greater speed will collide more frequently. Also, molecules collide with more energy, this makes it easier for bonds to be broken so that the reaction can occur. ...read more.


2.Using a 2ml syringe place 2ml of starch suspension into each of the five tubes. 3.Using another 2ml syringe place 2ml of amylase solution into each of other five tubes. 4.Pair up the test tubes of starch and amylase. 5.Put each pair of the test tubes into one of the water baths for at least 3 minutes. 6.For one pair, pour the amylase into the starch, shake and immediately start the stopclock. Simultaneously remove one drop of the mixture with a clean glass rod and test with orange iodine solution on a spotting tile. This should confirm that the starch is present and that at time| =0 seconds the amylase has not catalysed the breakdown of starch. ...read more.


Graph is plotted on the graph paper Interpretation The curve on the graph indicate that the rate of the reaction increases as the temperature increases from 0�C to 60�C and decreases as the temperature increases from 60�C to further. The increasing part is produced by the fact that high temperature increases the rate of the reaction. The decreasing part is produced by another important fact. At about 60�C, the enzyme is denatured by the extra energy provided. Denatured enzyme loses the ability of catalyzing the substrate. As the temperature goes higher than the optimum temperature (this enzyme has the optimum temperature about 60�C.), the percentage of denatured enzyme gets greater. So less and less enzyme molecules are available to work properly. In consequence, the higher the temperature above the optimum temperature, the slower the reaction ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Following the Progress of an Enzyme Controlled Reaction

    4 star(s)

    increase the rate at which they work and the rate of motion that they incur. The general optimum temperature at which enzyme are most suited to is at around 40-50 degrees c. However this varies from different enzymes and this optimum temperature bay fluctuate or is reduced.

  2. The effect of temperature on an enzyme controlled reaction

    Though if the temperature is too high (500C) the catalase enzymes will start to denature because the active site of the enzyme will become deformed and the particles of H2O2 will not be able to fit into the enzyme. Hence the molecules will not be broken down, decreasing the rate of reaction.

  1. Catalyse Investigation

    balanced the results or from the set of results that I got when I collected 20 ml at each concentration (rate 2). If I take these into account buyer have a very strange pattern which fire he cannot explain. They seem to generally show a trend of regular increase in

  2. Amylase Investigation

    This is thought to be due to the fact that there are so many substrate molecules competing for the active sites on the enzyme surfaces that they block the sites (Figure 9) and prevent any other substrate molecules from occupying them.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work