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Investigation of the effect of temperature on the enzyme controlled reaction

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Investigation of the effect of temperature on the enzyme controlled reaction Background The change of temperature will affect the rate of a reaction. This is because that for an amount of reactants, increase temperature will give it more heat energy and the molecules in it will have greater kinetic energy. For the mass of the molecules are not changed, these molecules will move with greater speed. (KE=1/2mv^) The reaction occurs when the reactant molecules collide with each other. The substrate molecules and enzyme molecules with greater speed will collide more frequently. Also, molecules collide with more energy, this makes it easier for bonds to be broken so that the reaction can occur. ...read more.


2.Using a 2ml syringe place 2ml of starch suspension into each of the five tubes. 3.Using another 2ml syringe place 2ml of amylase solution into each of other five tubes. 4.Pair up the test tubes of starch and amylase. 5.Put each pair of the test tubes into one of the water baths for at least 3 minutes. 6.For one pair, pour the amylase into the starch, shake and immediately start the stopclock. Simultaneously remove one drop of the mixture with a clean glass rod and test with orange iodine solution on a spotting tile. This should confirm that the starch is present and that at time| =0 seconds the amylase has not catalysed the breakdown of starch. ...read more.


Graph is plotted on the graph paper Interpretation The curve on the graph indicate that the rate of the reaction increases as the temperature increases from 0�C to 60�C and decreases as the temperature increases from 60�C to further. The increasing part is produced by the fact that high temperature increases the rate of the reaction. The decreasing part is produced by another important fact. At about 60�C, the enzyme is denatured by the extra energy provided. Denatured enzyme loses the ability of catalyzing the substrate. As the temperature goes higher than the optimum temperature (this enzyme has the optimum temperature about 60�C.), the percentage of denatured enzyme gets greater. So less and less enzyme molecules are available to work properly. In consequence, the higher the temperature above the optimum temperature, the slower the reaction ...read more.

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