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Lipase Investigation.

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Introduction

Biology Coursework - Lipase Investigation Aim Our aim is to investigate how the temperature affects the rate of reaction of the enzyme lipase. Equipment Apparatus -3cm� Milk solution -1cm� Lipase solution -2cm� Sodium carbonate solution -2 drops of Phenolphalene -Test Tubes Method 1. First we will set 6 test tubes each containing the ingredients above. 2. Next we will place them in water baths at 6 different temperatures. 3. Then record how long it takes for the tube to turn all white 4. We will then repeat the experiment three times for each temperature and take an average reading. Enzymes Enzymes are biological catalysts; they speed up the chemical reactions that happen in living cells. Enzymes carry out their job at a rapid rate. Enzymes are formed inside cells and after they are formed they leave their cells to carry out their jobs outside. Enzymes that act on lipids are called lipases. Examples of Enzymes are proteise, protein, lipase and lipids. The solution will turn white because when the lipase and milk get in contact they produce fatty acids and glycerol, so the ...read more.

Middle

2cm� 120s 3cm� 345s 4cm� 485s Fair test To make this a fair test we will: 1. Keep room temp 22 �C must keep constant because as little as a 10�C rise in temperature can half the time it takes the enzymes to break down the fats. 2. Lipase: A higher lipase concentration will obviously speed up the time it takes to break down the fat. 3. Use the same amount of ingredients. 4. Make sure to stir milk as lipase is not that soluble in water and does settle out. 5. Use the same timer. Key Factors The things that we will change are the temperature starting with 20�C and going up until we reach 70�C. The things we will keep the same are the amount of Milk, Phenolphalene, Lipase and Sodium Carbonate. We should also take into account the accuracy of measuring to ensure a fair test. Results Amount of time taken for fat to be broken down (seconds) Temperature (�C) Test 1 (s) ...read more.

Conclusion

Overall the experiment went quite well though my results are not completely accurate as it would have been hard to get the tube directly to the water bath as soon as possible. If we were to do the experiment again we would have taken a wider range of results to be more accurate and also keep a closer eye on the test tubes when comparing the colour change from cream to white. Another factor would have been the timing, the water baths were sometimes placed far away so the reaction could have started while the tube was in air temperature not 60 or 70 �C. The evidence collected is quite accurate after doing 3 tests for each temperature the outcome was what I expected in our prediction. We only had 1 or 2 anomalies with the odd high or low reading because of where the water baths were placed in the room, so it was hard to get a 100% accurate experiment. Our additional preliminary work carried out helped us on deciding an important factor of the volume of Sodium Carbonate to use. ...read more.

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