Lipase Investigation.

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Biology Coursework - Lipase Investigation

Aim

Our aim is to investigate how the temperature affects the rate of reaction of the enzyme lipase.

Equipment                                                        Apparatus
-3cm³ Milk solution
-1cm³ Lipase solution
-2cm³ Sodium carbonate solution
-2 drops of Phenolphalene
-Test Tubes

Method

  1. First we will set 6 test tubes each containing the ingredients above.
  2. Next we will place them in water baths at 6 different temperatures.
  3. Then record how long it takes for the tube to turn all white
  4. We will then repeat the experiment three times for each temperature and take an average reading.

Enzymes

Enzymes are biological catalysts; they speed up the chemical reactions that happen in living cells. Enzymes carry out their job at a rapid rate. Enzymes are formed inside cells and after they are formed they leave their cells to carry out their jobs outside. Enzymes that act on lipids are called lipases. Examples of Enzymes are proteise, protein, lipase and lipids.

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The solution will turn white because when the lipase and milk get in contact they produce fatty acids and glycerol, so the Sodium Carbonate makes the solution alkaline which is pink to start with but as more acids are produced the pH drops and the solution turns white.

Prediction

I predict that the higher the temperature, the less time the whole process will take. I think this because when there is more energy, each fat molecule will be able to find an enzyme faster so then the enzyme can break it down into fatty acids and glycerol. A higher temperature ...

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