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Liver and Hydrogen Peroxide experiment

  • Essay length: 1625 words
  • Submitted: 11/03/2007
  • Marked by teacher: (?) Ross Robertson
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AS and A Level Molecules & Cells

Teacher essay summary

3 star(s)

Overall, the investigation is based on sound biochemistry and the author demonstrates at least a partial understanding of the factors affecting reaction rates in living organisms. The interaction of substrate and enzyme concentration needed clarification at the start.
Substrate concentration was not calculated at any stage, a surprising omission.
The DV was not specifically addressed in the report and readers will be a little confused by this.
The data collected was not wholly accurate or reproduceable, almost certainly due to the preparation of the liver.
A fair effort overall, but with a number of key weaknesses that would certainly undermine the overall grade.

Marked by teacher Ross Robertson 01/03/2013

The first 200 words of this essay...

Liver and Hydrogen Peroxide experiment

Aim: To see how different concentrations of hydrogen peroxide affects the enzyme activity and rate of reaction.

Hypothesis: I believe that when the concentration of the substrate (hydrogen peroxide) increases this will cause the rate of reaction to increase.

I believe this because enzyme activity and substrate concentration affect the rate of reaction. As the concentration of enzymes increase so does the number of active sites. If there is excess substrates present. This will then cause the rate of reaction to increase in the proportion of excess substrate molecules and enzymes. The rate of reaction depends on the rate of formation of enzyme - substrate complexes.

As the substrate concentration increases the rate of reaction will increase until all enzyme molecules are being used up. After this point it doesn't matter how much more substrate you add the rate of reaction will not increase anymore as the enzymes are working as fast as they can to break down the substrate. The rate reaches maximum velocity and remains constant. Basically with the highest concentration the enzymes will be able to collide with more substrate molecules.

Here are

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MbT essay summary

Overall, the investigation is based on sound biochemistry and the author demonstrates at least a partial understanding of the factors affecting reaction rates in living organisms. The interaction of substrate and enzyme concentration needed clarification at the start.
Substrate concentration was not calculated at any stage, a surprising omission.
The DV was not specifically addressed in the report and readers will be a little confused by this.
The data collected was not wholly accurate or reproduceable, almost certainly due to the preparation of the liver.
A fair effort overall, but with a number of key weaknesses that would certainly undermine the overall grade.

Marked by teacher Ross Robertson 01/03/2013

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