• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Membrane permeability in beetroot cells.

Extracts from this document...

Introduction

Membrane Permeability in Beetroot cells Beet root cells contains a red pigment, which is found in the cell vacuole. The vacuole has a membrane in which its function is mainly to prevent the betacyanins from leaving the cell. Leakage of these betacyanins into the external solution can be used as an indication of membrance pereambility changes. As part of my experiment, my aim is to see if temperature affects the membrane by witnessing the amount of betacynanins that leaks from the beetroot cells. The cell membrane covers the outside of a cell and consists of a double layered sheet of lipid molecules interspered with proteins. It seperates the cell from the external environment, gives phyisical protection and allows the import and export of selected chemicals. Aim: I will commence the experiment by placing beetroots in a test tube of deionised water, I will use a range of temperatures in which I will place the beetroot cell to see the damage later on. After placing them in series of test tubes of deionised water for 30 minutes. ...read more.

Middle

* Measuring cylinder: The amount of deionised water in each test tube has be exactly the same. Otherwise this will create an error in my experiment. Therefore using a measuring cylinder enable my experiment to be fair and accurate. Apparatus: * Goggles, � Ruler * Lab coat �Colorimeter * Beetroot � Petri dish * Cork borer �Tile * Marker � Spatula * Test tubes * Labels * Test tube holder * Deionised water * Thermometer * Measuring cylinder * Cuvette Fair Testing: In this experiment, a fair testing is crucial, as it will indicate whether my results were accurate or inaccurate. I must also ensure all factors are kept the same throughout my practical. Results: Membrane Permeability Table Beet root number Temperature (degrees Celsius) Colorimeter (% Transmission) 1/ % transmission 1 80 64 0.015 2 61 75 0.013 3 42 87 0.011 4 23 96 0.010 5 10 100 0.01 Conclusion, improvements and Evaluation: My experiment is now complete, and I have witnessed a pattern in my observations. ...read more.

Conclusion

The beetroot cells did not stay in the test tubes for 30 minutes approxiamately. This could mean the colour may not have had enough time to diffuse out of the beetroot cells. Hence creating an error in my observations. An error could have also risen from the fact that the beetroots not washed properly . It was also important to have washed out the pigment from the cells that were damaged when using the cork borer. The anomaly I would say was the sample that was placed in a 5�c bath. Which gave me a relatively large percentage of beetroot pigment concentration, due to the fact that the cell membrane could not work at such a cold tempertaure, hence therefore the pigment (betacyanin) was easily diffused. On the other hand despite the fact I made a number of errors during the practical and the fact that I did the experiment alone and was under pressure to complete the practical on time. I can truthfully say I did this practical to the best of my ability. I believe with more practise, I will get the hang of it and next time I would be able to produce a better results. Donald Blankson Hemans - 1 - ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay


**
A weak experimental report that lacks the depth required to gain good marks in an A level course. Although some results were obtained the key sections were either missing or lacking in key details.
To improve:
Research and Rationale
There needs to be background research carried out and discussed with respect to the likely effect of temperature on the components of a plasma membrane. The references used to inform the plan should be cited.
Planning
Although a list of apparatus was present there was no written method and no risk assessment. The key variables need to be listed and the way they will be controlled explained. There was no pilot experiment carried out.
Recording
There were some results recorded but no replicates were carried out. The table headings were unclear in some cases.
Interpreting and Evaluating
The interpretation of the results was not linked to biological knowledge and the limitations of the results were not fully discussed. The evaluation was mainly a list of personal failings with regard to measuring and lack of time which would fail to gain credit. The student would need to look more critically at the method ( unfortunately not in the report) and discuss ways that reliability could be improved or the investigation extended.

Marked by teacher Stevie Fleming 01/01/1970

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    1 Thermometer. 100cm� glass beaker. Laminated card with a marking on it. 1 Stop Clock. Some ice cubes. 1 Tray. 1 Water Bath. Goggles Gloves Outline of Experiment First we will make sure we have all of the equipment listed above on our desk and laid out correctly so that

  2. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    [18] The S-H group is called the sulphydryl group. This group can be located in the active site or elsewhere. Copper ions will combine permanently to the sulphydryl (-SH) groups. This could be in the active site or elsewhere [13] of the enzyme.

  1. Marked by a teacher

    Beetroot Experiment. The purpose of this experiment is to determine the effect of temperature ...

    4 star(s)

    Independent variable = Betalain pigment as measured by colorimeter This is the variable that I am trying to measure in order to determine the structural integrity of the plasma membrane. Constant variables In order to make this a fare teat and to ensure that we are measuring the effect of

  2. Marked by a teacher

    The Effect of Ethanol Concentration on the Permeability of Beetroot Cell Membranes to Betalain

    3 star(s)

    Variable Time of immersion of boiling tubes in the thermostatically controlled water bath for each concentration. Reason why it must be kept constant in the context of this experiment From watching my preliminary experiment I can see that as time progresses more and more betalain diffuses out of the cell membrane.

  1. To find out how different concentrations of sucrose solution affect the incipient plasmolysis of ...

    I originally predicted that the isotonic point would occur at a molarity of 0.5M. This was the point at which the solute potential was equal to the water potential and therefore water moves in and out of the cell in equal rates.

  2. Permeability of Cell Membrane in Beetroot Cells

    As the temperature increased, the rate of reaction also increased until it reached a point. Once it hit a temperature of about 60?C the rate decreased as the shape of the enzyme's active site was changed and damaged irreparably, this was due to Denaturation.

  1. Why does the colour leak out of cooked beetroot?

    control as well as doing the following things: ?I will leave the boiling tubes of water in the water baths for 5 minutes before I add the beetroot pieces to them, so that they have reached the required temperatures. ?I will leave the boiling tubes containing water and beetroot in

  2. An experiment to find of the isotonic point of root vegetables cells in contents ...

    Also will be used label the water and 1Molar sucrose solution beakers. This will prevent my results from becoming mixed up. Will also be used to label the pipettes. * Scalpel- This will be used to cut the root vegetable to the accurate and appropriate length, this will minimize the

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work