• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Membrane Permeability : the effect of temperature

Extracts from this document...

Introduction

Membrane Permeability : the effect of temperature AFTAB MOHAMMED Membrane Permeability : the effect of temperature TITLE 'How does temperature effect membrane permeability?' METHOD The Investigation was carried out in the following way: * Six pieces of beetroot were cut out about 2 cms long using a cork borer. * The cylinders of beetroot were placed on a white tile and using a razor blade they were cut into discs 3 mm thick. * The discs were placed in a beaker of tap water and washed thoroughly, rinsing out the beaker several times. * Test tubes were labelled for each temperature to be tested. The temperatures required were 30, 40, 50, 60, 70 and 80?c * 6cm� of tap water was put in each test tube * 6 washed beetroot discs were impaled on a mounted needle allowing space between each disc. * Mounted needle with discs was placed in the 30?c water bath for exactly one minute. At the end of this time, the discs were taken off the needle and dropped into the test tube labelled 30?c * This procedure was repeated for temperatures 40, 50, 60, 70 and 80?c. ...read more.

Middle

Between 40-50?c, the opposite happens here, my results show that the absorbance decreases but the mean results show that the absorbance increases Between 50-60?c, there was an increase in the absorbance Between 60-70?c, there was a very steep increase in the absorbance Between 70-80?c, there was an increase in both results The difference in the results at the beginning of the graph was probably due to anomalous results. CONCLUSION The graph clearly shows that as the temperature increases, the absorbency also increases. The beetroot discs were washed in the experiment to remove any anthocyonin from the surface. The colour of the liquid in the test tubes changed due to diffusion taking place. Anthocyonin diffused out from the cell into the liquid. This happened because of the big concentration gradient. The results showed that as the temperature was increased the membrane became more permeable and allowed more pigment to enter the liquid from the beetroot. DISCUSSION The cell membrane contains intrinsic proteins that control the membrane's selective permeability. ...read more.

Conclusion

structure. This structure accounts for the cell letting the anthocyonin out. EVALUATION Errors: * Some beetroot may have more anthocyonin than others. This is trivial though; it would not make much difference. * The beetroot discs were not measured as accurately as they seemed. They could be significantly too large or small. * The equipment used was not accurate enough. Using a pipette is not necessarily accurate; it could have produced errors in measurement. Limitations: * The whole experiment should be repeated more times to make the results more reliable * Only 6 temperatures were looked at, we could have done more * When the beetroot was stabbed to put on the needle, it could have allowed anthocyonin to come out. But this was done so that the beetroot discs don't touch one another and maximum surface area is created. * Errors could have been made when using the colorimeter, it as a machine and they can make accuracy errors. * Cuvettes had to be handled very carefully, but scratches may have already been on then and fingerprints also affect the absorbency. So in effect, these could have taken place. AFTAB Mohammed BIOLOGY SALTERS 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigating the effect of temperature on the movement of pigment through beetroot cell membranes.

    4 star(s)

    PROCEDURE: 1. Heat 200 cm� of distilled water to 80?C. 2. Whilst water is heating place 10 cm� of distilled water into 15 sample tubes labeling them like so: 80?C, 70?C, 60?C, 50?C, 40?C, and there are three pots to be labeled at each temperature.

  2. Marked by a teacher

    Effect of different temperatures on the permeability of beetroot cell membrane.

    3 star(s)

    Or use a different piece of equipment rather than a knife such as a mini plastic mitre block or something similar to a egg slice. So there is less chance of the beetroot being cut vertically if it is not rushed.

  1. Marked by a teacher

    effect of temperature on beetroot pigment through membrane

    3 star(s)

    For the experiment with ice-cold water, fill the beaker quarter with tap water and add ice-cubes. Allow 5minutes for the water to cool down to the minimum temperature. 5. After the specified temperature is reached, place 3 beetroot disks into each boiling tube and leave for 3 minutes, time with a stop watch.

  2. Marked by a teacher

    An investigation to examine the effects of temperature on membrane stability in beetroot, by ...

    3 star(s)

    These particular R-groups will only allow certain molecules or ions to pass through, depending upon which R-groups line the inside of the protein. So, once a protein becomes denatured, some of these R-groups may move away from the lining and different R-groups may take their place.

  1. Marked by a teacher

    AN INVESTIGATION INTO HOW TEMPERATURE AFFECTS THE PERMEABILITY MEMBRANE OF A BEETROOT.

    3 star(s)

    This is because the temperature is too low, resulting in the beetroot's particles having less kinetic enerygy, with the betalain pigment having less energy for a collision to occur.

  2. Marked by a teacher

    Effect Of Detergent Concentration On Membrane Permeability Of Beetroot Cells

    or it may be done near the heater, this could damage the results, because a higher temperature could mean the membrane is broken due to the heat, and not solely the detergent, An increase in temperature will also damage and denature the plasma membrane and cause the cytoplasm and other

  1. TEMPERATURE ON BEETROOT PERMEABILITY

    The majority belong to one subgroup of triglycerides known as phospholipids. Phospholipids make up the basic structure of the membrane, forming a bi layer. They have hydrophilic heads and hydrophobic tails, with the tales being non polar it is very difficult for ions and polar molecules to pass through the

  2. An experiment to test the effect of different temperatures on the permeability of cell ...

    more permeable with increasing temperature is that the protein would get denatured. This means that temperature and permeability are not in direct proportion, but the ratio would change when it gets above 40�C when permeability increase more per amount of temperature increased.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work