microbiology ph and temperature effect on bacterial culture

The first 200 words of this essay...

Aim of this experiment is to investigate the effect of pH and temperature on bacteria growth.

Apparatus: 4 universal bottles at different pH's, Escherichia coli, micrococcus luteus, pipettes, incubator, 4 test tubes, colorimeter.

Method of pH

Four bottles where used which contained nutrient growth at pH 5, 6, 7, 8. By using aseptic techniques, a pipette is used as each nutrient broth is inoculated with one drop of culture Escherichia coli. After this each of the nutrient broth was incubated at a temperature of 25 degrees for 3 days. After 3 days the contents within each of the bottle are mixed and their contents are transferred into a test tube. At first each pH condition is set to absorbance to zero by using a nutrient broth without any bacterial culture. After this was done the nutrient broth which contained the bacteria is measured to find out it's absorbance by using a colorimeter. This procedure is done with the micrococcus luteus bacteria.

These results are shown in a table below of the effect of growth of bacteria Escherichia coli

pH range

pH4

pH5

pH6

pH7

Absorbance group 1